Neuritogenic Effect of Epolactaene Derivatives on Human Neuroblastoma Cells Which Lack High-Affinity Nerve Growth Factor Receptors

1997 ◽  
Vol 40 (4) ◽  
pp. 391-394 ◽  
Author(s):  
Hideaki Kakeya ◽  
Chizuko Onozawa ◽  
Masakazu Sato ◽  
Koshi Arai ◽  
Hiroyuki Osada
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Neuroblastoma Cells ◽  
Growth Factor Receptors ◽  
Human Neuroblastoma Cells ◽  
Human Neuroblastoma ◽  
Nerve Growth ◽  
High Affinity ◽  
Nerve Growth Factor Receptors

scholarly journals The transcription of the human fructose-bisphosphate aldolase C gene is activated by nerve-growth-factor-induced B factor in human neuroblastoma cells*

1997 ◽  
Vol 323 (1) ◽  
pp. 245-250 ◽  
Author(s):  
Pasqualina BUONO ◽  
Lisa de CONCILIIS ◽  
Paola IZZO ◽  
Francesco SALVATORE
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Neuroblastoma Cells ◽  
Neuronal Cell ◽  
Fructose Bisphosphate ◽  
Human Neuroblastoma Cells ◽  
Human Neuroblastoma ◽  
Nerve Growth ◽  
Aldolase C ◽  
Fructose Bisphosphate Aldolase

A DNA region located at around -200 bp in the 5´ flanking region (region D) of the human brain-type fructose-bisphosphate aldolase (aldolase C) gene has been analysed. We show by transient transfection assay and electrophoretic-mobility-shift assay (EMSA) that the binding of transcriptional activators to region D is much more efficient (80% versus 30%) in human neuroblastoma cells (SKNBE) than in the non-neuronal cell line A1251, which contains low levels of aldolase C mRNA. The sequence of region D, CAAGGTCA, is very similar to the AAAGGTCA motif present in the mouse steroid 21-hydroxylase gene; the latter motif binds nerve-growth-factor-induced B factor (NGFI-B), which is a member of the thyroid/steroid/retinoid nuclear receptor gene family. Competition experiments in EMSA and antibody-directed supershift experiments showed that NGFI-B is involved in the binding to region D of the human aldolase C gene. Furthermore, the regulation of the aldolase C gene (which is the second known target of NGFI-B) expression during development parallels that of NGFI-B.

Nerve growth factor may function as a survival factor for human neuroblastoma cells in culture

Cancer ◽  
1984 ◽  
Vol 54 (7) ◽  
pp. 1344-1347 ◽  
Author(s):  
Arthur S. Tischler ◽  
Val W. Slayton ◽  
Donna S. Costopoulos ◽  
Lucian L. Leape ◽  
Ronald A. Delellis ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Neuroblastoma Cells ◽  
Human Neuroblastoma Cells ◽  
Human Neuroblastoma ◽  
Nerve Growth ◽  
Cells In Culture ◽  
Survival Factor
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