Discovery and Optimization of Chromenotriazolopyrimidines as Potent Inhibitors of the Mouse Double Minute 2−Tumor Protein 53 Protein−Protein Interaction

2009 ◽  
Vol 52 (22) ◽  
pp. 7044-7053 ◽  
Author(s):  
John G. Allen ◽  
Matthew P. Bourbeau ◽  
G. Erich Wohlhieter ◽  
Michael D. Bartberger ◽  
Klaus Michelsen ◽  
...  
Keyword(s):  
Protein Interaction ◽  
Protein Protein Interaction ◽  
Mouse Double Minute ◽  
Double Minute

scholarly journals Recent Small-Molecule Inhibitors of the p53–MDM2 Protein–Protein Interaction

Molecules ◽  
2020 ◽  
Vol 25 (5) ◽  
pp. 1211 ◽  
Author(s):  
Anastasia Beloglazkina ◽  
Nikolai Zyk ◽  
Alexander Majouga ◽  
Elena Beloglazkina
Keyword(s):  
Small Molecule ◽  
Protein Interaction ◽  
Small Molecule Inhibitors ◽  
Mdm2 Protein ◽  
Binding Characteristics ◽  
Protein Protein Interaction ◽  
Mouse Double Minute ◽  
Double Minute ◽  
Synthetic Approaches

This review presents the last decade of studies on the synthesis of various types of small-molecule inhibitors of the p53– Mouse double minute 2 homolog (MDM2) protein–protein interaction. The main focus is placed on synthetic approaches to such molecules, their cytotoxicity, and MDM2 binding characteristics.

An efficient transient assays system using Agrobacterium-mediated transformation of onion (Allium cepa) epidermal cells

2020 ◽  
Vol 80 (03) ◽  
Author(s):  
Yu-Miao Zhang ◽  
Jun Wang ◽  
Tao Wu
Keyword(s):  
Subcellular Localization ◽  
Protein Interaction ◽  
Protein Interactions ◽  
Epidermal Cells ◽  
Cyclin Dependent Kinase ◽  
Protein Subcellular Localization ◽  
Protein Protein Interactions ◽  
Efficient System ◽  
Protein Protein Interaction ◽  
Onion Epidermal Cells

In this study, the Agrobacterium infection medium, infection duration, detergent, and cell density were optimized. The sorghum-based infection medium (SbIM), 10-20 min infection time, addition of 0.01% Silwet L-77, and Agrobacterium optical density at 600 nm (OD600), improved the competence of onion epidermal cells to support Agrobacterium infection at >90% efficiency. Cyclin-dependent kinase D-2 (CDKD-2) and cytochrome c-type biogenesis protein (CYCH), protein-protein interactions were localized. The optimized procedure is a quick and efficient system for examining protein subcellular localization and protein-protein interaction.

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