Spectrophotometric Method for the Determination of Chlorpromazine and Chlorpromazine Sulphoxide in Biological Fluids

1959 ◽  
Vol 1 (3) ◽  
pp. 263-273 ◽  
Author(s):  
T. L. Flanagan ◽  
T. H. Lin ◽  
W. J. Novick ◽  
I. M. Rondish ◽  
C. A. Bocher ◽  
...  
Keyword(s):  
Spectrophotometric Method ◽  
Biological Fluids

A More Sensitive Spectrophotometric Method for Determination of Isoniazid in Serum or Plasma

1971 ◽  
Vol 17 (6) ◽  
pp. 492-494 ◽  
Author(s):  
Leslie Eidus ◽  
Anita M T Harnanansingh
Keyword(s):  
Hydrochloric Acid ◽  
Organic Solvents ◽  
Spectrophotometric Method ◽  
Biological Fluids ◽  
Coupling Reagent ◽  
Sensitive Spectrophotometric Method

Abstract A sensitive spectrophotometric method is described for determining isoniazid in serum or plasma. It is extracted from biological fluids by a mixture of organic solvents and re-extracted into hydrochloric acid. In this medium, isoniazid reacts with trans-cinnamaldehyde to form a derivative absorbing maximally at 340 nm. The method is five times more sensitive than published procedures in which vanillin or piperonal is used as coupling reagent for the estimation of isoniazid.

scholarly journals A highly sensitive and selective spectrophotometric method for the determination of vanadium at nanotrace levels in some environmental, biological, soil, food, and pharmaceutical samples using salicylaldehyde-benzoylhydrazone

2020 ◽  
Vol 11 (4) ◽  
pp. 385-395
Author(s):  
Shaifa Abrarin ◽  
Mohammed Jamaluddin Ahmed
Keyword(s):  
Spectrophotometric Method ◽  
Biological Fluids ◽  
Stoichiometric Composition ◽  
Standard Reference Materials ◽  
Complexing Agents ◽  
Environmental Waters ◽  
Pharmaceutical Samples ◽  
Highly Sensitive ◽  
Interference Study

A very simple, non-extractive and new spectrophotometric method for the swift determination of trace amount of vanadium using salicylaldehyde-benzoylhydrazone (Sal-BH) has been developed. Sal-BH undergoes a reaction in a slightly acidic solution (0.0016-0.0032 M H2S04) with vanadium to give a light greenish-yellow chelate, which has an absorption maximum at 392 nm. The reaction is instantaneous and absorbance remains stable for over 24 hrs. The average molar absorption co-efficient and Sandell’s sensitivity were found to be 2.5039×105 L/mol.cm and 1.0 ng/cm2 V, respectively. Beer’s law was obeyed for 0.001-30 mg/L of V, providing a detection limit of 0.1 µg/L of V and RSD 0-2 %. The stoichiometric composition of the chelate is 1:1 (V:Sal-BH). Interference study shows that a large excess of over 60 cations, anions, and some common complexing agents (such as chloride, azide, tartrate, EDTA and SCN‑, etc.) satisfy the tolerance limit. The developed method was successfully used in the determination of vanadium in several standard reference materials as well as in some environmental waters, biological fluids, soil, food and pharmaceutical samples and solutions containing both vanadium (IV) and vanadium (V). The results of the proposed method for assessing biological, food and vegetable samples were comparable with ICP-OES and AAS were found to be in excellent agreement. The method has high precision and accuracy (s = ±0.01 for 0.5 mg/L).

scholarly journals Development of a new highly sensitive and selective spectrophotometric method for the determination of selenium at nano-trace levels in various complex matrices using salicylaldehyde-orthoaminophenol

2021 ◽  
Vol 12 (4) ◽  
pp. 469-481
Author(s):  
Muhammad Jamaluddin Ahmed ◽  
Muhammad Jihan Uddin ◽  
Muhammad Emdadul Hoque
Keyword(s):  
Spectrophotometric Method ◽  
Certified Reference Materials ◽  
Biological Fluids ◽  
Stoichiometric Composition ◽  
Bovine Liver ◽  
Food Samples ◽  
Soil Samples ◽  
Complexing Agents ◽  
Linear Calibration

A new spectrophotometric reagent, salicylaldehyde-orthoaminophenol (Sal-OAP) has been synthesized and characterized for the determination of selenium through novel reaction techniques. Also, a new highly selective, and sensitive spectrophotometric method for the nano-trace determination of selenium using salicylaldehyde-orthoaminophenol (Sal-OAP) has been developed. Sal-OAP undergoes reaction in a slightly acidic solution (0.0001-0.0002 M H2S04) with selenium (IV) to give an orange-red chelate, which has an absorption maximum at 379 nm. The reaction is instantaneous and absorbance remains stable for over 24 h. The average molar absorption co-efficient and Sandell’s sensitivity were found to be 6.4×105 L/mol.cm and 1.0 ng/cm2 of, respectively. Linear calibration graphs were obtained for 0.001-40.000 mg/Lof Se having detection limit of 0.1 µg/L and RSD 0-2 %. The stoichiometric composition of the chelate is 1:2 (Se:Sal-OAP). A large excess of over 60 cations, anions and some common complexing agents, such as chloride, azide, tartrate, EDTA, SCN¯etc., do not interfere in the determination. The developed method was successfully used in the determination of selenium in several Certified Reference Materials (Alloys, steels, human urine, bovine liver, drinking water, tea, milk, soil, and sediments) as well as in some environmental waters (Potable and polluted), biological fluids (Human blood, urine, hair, and milk), soil samples, food samples (Vegetables, rice, and wheat) and pharmaceutical samples (Tablet and syrup) and solutions containing both selenium (IV) and selenium (VI) as well as complex synthetic mixtures. The results of the proposed method for assessing biological, food and vegetables and soil samples were comparable with ICP-OES and AAS were found to be in excellent agreement. The method has high precision and accuracy (s = ±0.01 for 0.5 mg/L).

Sign in / Sign up

Export Citation Format

Share Document