Discovery of Novel Inhibitors Targeting the Macrophage Migration Inhibitory Factor via Structure-Based Virtual Screening and Bioassays

2014 ◽  
Vol 57 (9) ◽  
pp. 3737-3745 ◽  
Author(s):  
Lei Xu ◽  
Yu Zhang ◽  
Longtai Zheng ◽  
Chunhua Qiao ◽  
Youyong Li ◽  
...  
Keyword(s):  
Virtual Screening ◽  
Migration Inhibitory Factor ◽  
Macrophage Migration Inhibitory Factor ◽  
Inhibitory Factor ◽  
Macrophage Migration

scholarly journals Discovery of Human Macrophage Migration Inhibitory Factor (MIF)-CD74 Antagonists via Virtual Screening

2009 ◽  
Vol 52 (2) ◽  
pp. 416-424 ◽  
Author(s):  
Zoe Cournia ◽  
Lin Leng ◽  
Sunilkumar Gandavadi ◽  
Xin Du ◽  
Richard Bucala ◽  
...  
Keyword(s):  
Virtual Screening ◽  
Migration Inhibitory Factor ◽  
Macrophage Migration Inhibitory Factor ◽  
Inhibitory Factor ◽  
Human Macrophage ◽  
Macrophage Migration

scholarly journals Virtual Screening of Some Active Human Macrophage Migration Inhibitory Factor Antagonists

2014 ◽  
Vol 19 (7) ◽  
pp. 1116-1123 ◽  
Author(s):  
Tsung-Lin Tsai ◽  
Thy-Hou Lin
Keyword(s):  
Virtual Screening ◽  
Migration Inhibitory Factor ◽  
Macrophage Migration Inhibitory Factor ◽  
Inhibitory Factor ◽  
Human Macrophage ◽  
Macrophage Migration ◽  
Active Compounds ◽  
Zinc Database ◽  
Docking Method ◽  
Better Than

Macrophage migration inhibitory factor (MIF) is an autocrine- and paracrine-acting cytokine that is involved in several inflammatory, autoimmune, infectious, and oncogenic diseases. Clinical data have shown that inhibition of MIF, especially its tautomerase activity, with small compounds has been beneficial in some disease models. A virtual screening (VS) experiment is conducted for searching some active compounds to inhibit the tautomerase activity of MIF from the ZINC database. By using an x-ray–determined structure OXIM-11 as the query and an in-house developed two-dimensional scaffold comparing method designated as Sfilter, we have screened out some 1500 compounds for ranking by our previously published docking method ADDock. After further ranking by ADDock on 119 compounds screened, we have decided to choose 17 of them for measuring their inhibitory activity IC50 against the MIF tautomerase experimentally. The IC50’s are measured using both human monocytic THP-1 cell lysate and purified recombinant human MIF protein. We have found that the IC50’s measured for three searched compounds (namely, ZINC02693801, ZINC00141102, and ZINC12368346) are better than that determined for ISO-1, a known MIF tautomerase inhibitor and standard used throughout our VS experiment. Moreover, the scaffolds of most of our active compounds searched are also quite different from those searched and published by others previously.

287: Macrophage Migration Inhibitory Factor and its Receptor CD74 are Upregulated in the Urothelium of Interstitial Cystitis Patients

2006 ◽  
Vol 175 (4S) ◽  
pp. 95-96 ◽  
Author(s):  
Pedro L. Vera ◽  
Kenneth A. lczkowski ◽  
Robert M. Moldwin ◽  
Leslie Kushner ◽  
Katherine L. Meyer-Siegler
Keyword(s):  
Interstitial Cystitis ◽  
Migration Inhibitory Factor ◽  
Macrophage Migration Inhibitory Factor ◽  
Inhibitory Factor ◽  
Macrophage Migration
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