scholarly journals Antitrypanosomal Lead Discovery: Identification of a Ligand-Efficient Inhibitor of Trypanosoma cruzi CYP51 and Parasite Growth

2013 ◽  
Vol 56 (6) ◽  
pp. 2556-2567 ◽  
Author(s):  
Grasiella Andriani ◽  
Emanuele Amata ◽  
Joel Beatty ◽  
Zeke Clements ◽  
Brian J. Coffey ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Parasite Growth ◽  
Lead Discovery ◽  
Efficient Inhibitor

Trypanosoma cruzi Polyamine Transporter: Its Role on Parasite Growth and Survival Under Stress Conditions

2016 ◽  
Vol 249 (4) ◽  
pp. 475-481 ◽  
Author(s):  
Chantal Reigada ◽  
Melisa Sayé ◽  
Edward Valera Vera ◽  
Darío Balcazar ◽  
Laura Fraccaroli ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Parasite Growth ◽  
Stress Conditions ◽  
Growth And Survival ◽  
Polyamine Transporter

scholarly journals Host Cell Lipid Bodies Triggered by Trypanosoma cruzi Infection and Enhanced by the Uptake of Apoptotic Cells Are Associated With Prostaglandin E2 Generation and Increased Parasite Growth

2011 ◽  
Vol 204 (6) ◽  
pp. 951-961 ◽  
Author(s):  
Heloisa D’Avila ◽  
Célio G. Freire-de-Lima ◽  
Natalia R. Roque ◽  
Livia Teixeira ◽  
Christina Barja-Fidalgo ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Prostaglandin E2 ◽  
Host Cell ◽  
Parasite Growth ◽  
Apoptotic Cells ◽  
Lipid Bodies ◽  
Cell Lipid ◽  
Cruzi Infection

Growth of Trypanosoma cruzi in vitro: development and application of a continuous-flow culture system

Parasitology ◽  
1982 ◽  
Vol 84 (3) ◽  
pp. 511-526 ◽  
Author(s):  
G. T. Williams ◽  
L. Hudson
Keyword(s):  
Growth Rate ◽  
Trypanosoma Cruzi ◽  
Continuous Flow ◽  
Culture System ◽  
Extreme Values ◽  
Environmental Parameters ◽  
Optimal Growth ◽  
Parasite Growth ◽  
Vitro Development

SummaryThe design and operation of a modular, bacteriological continuous-flow culture system have been adapted for the growth of Trypanosoma cruzi epimastigotes in simple monophasic media. The system was designed to achieve a minimum of 200 days of continuous culture and provision was made for the continuous supply of medium and collection of parasites under sterile conditions. The system provides large quantities of epimastigotes with homogeneous morphology and uniform viability. The system also lends itself tothe analysis of the factors which affect parasite growth. We have examined the effects of changes in environmental parameters on epimastigote growth rate. Optimal growth was observed at 27 °C. The rate ofstirring of the culture had a small but definable effect on the growth rate, which was greatest at 80 r.p.m. Growth was only slightly affected by the level of dissolved oxygen between 10 and 50% saturation, but was inhibited at higher concentrations. Growth was slower at extreme values of pH but showed a broad optimum around pH 7·4.

scholarly journals A Novel Trypanosoma cruzi Protein Associated to the Flagellar Pocket of Replicative Stages and Involved in Parasite Growth

PLoS ONE ◽  
2015 ◽  
Vol 10 (6) ◽  
pp. e0130099 ◽  
Author(s):  
Ignacio M. Durante ◽  
María de los Milagros Cámara ◽  
Carlos A. Buscaglia
Keyword(s):  
Trypanosoma Cruzi ◽  
Parasite Growth ◽  
Flagellar Pocket

Trypanosoma cruzi: Inhibition of Parasite Growth and Respiration by Oxazolo(thiazolo)pyridine Derivatives and Its Relationship to Redox Potential and Lipophilicity

2001 ◽  
Vol 99 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Juan Diego Maya ◽  
Antonio Morello ◽  
Yolanda Repetto ◽  
Andrés Rodrı́guez ◽  
Pilar Puebla ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Redox Potential ◽  
Parasite Growth ◽  
Pyridine Derivatives

scholarly journals Anti-Trypanosoma cruzi Activity of Metabolism Modifier Compounds

2021 ◽  
Vol 22 (2) ◽  
pp. 688 ◽  
Author(s):  
Nieves Martinez-Peinado ◽  
Clara Martori ◽  
Nuria Cortes-Serra ◽  
Julian Sherman ◽  
Ana Rodriguez ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Developmental Stages ◽  
Cell Metabolism ◽  
Heat Shock Protein 90 ◽  
Inhibitory Effect ◽  
Parasite Growth ◽  
New Drugs ◽  
In Vitro Assays ◽  
Growth Environment

Chagas disease is caused by the protozoan parasite Trypanosoma cruzi and affects over 6 million people worldwide. Development of new drugs to treat this disease remains a priority since those currently available have variable efficacy and frequent adverse effects, especially during the long regimens required for treating the chronic stage of the disease. T. cruzi modulates the host cell-metabolism to accommodate the cell cytosol into a favorable growth environment and acquire nutrients for its multiplication. In this study we evaluated the specific anti-T. cruzi activity of nine bio-energetic modulator compounds. Notably, we identified that 17-DMAG, which targets the ATP-binding site of heat shock protein 90 (Hsp90), has a very high (sub-micromolar range) selective inhibition of the parasite growth. This inhibitory effect was also highly potent (IC50 = 0.27 μmol L−1) against the amastigote intracellular replicative stage of the parasite. Moreover, molecular docking results suggest that 17-DMAG may bind T. cruzi Hsp90 homologue Hsp83 with good affinity. Evaluation in a mouse model of chronic T. cruzi infection did not show parasite growth inhibition, highlighting the difficulties encountered when going from in vitro assays onto preclinical drug developmental stages.

scholarly journals Intracellular Growth of Trypanosoma cruzi in Cardiac Myocytes Is Inhibited by Cytokine-Induced Nitric Oxide Release

2004 ◽  
Vol 72 (1) ◽  
pp. 359-363 ◽  
Author(s):  
Laura Edith Fichera ◽  
Maria Cecilia Albareda ◽  
Susana Adriana Laucella ◽  
Miriam Postan
Keyword(s):  
Nitric Oxide ◽  
Trypanosoma Cruzi ◽  
Cardiac Myocytes ◽  
Cardiac Myocyte ◽  
Parasite Growth ◽  
Inos Expression ◽  
No Production ◽  
Necrosis Factor Alpha ◽  
Tnf Α ◽  
Ifn Γ

ABSTRACT The effect of interleukin-1β (IL-1β), tumor necrosis factor alpha (TNF-α), and gamma interferon (IFN-γ) on Trypanosoma cruzi multiplication and nitric oxide (NO) production in cardiac myocytes was investigated. Cardiac myocyte cultures were obtained from neonatal Wistar rat hearts, infected with T. cruzi, and treated with IL-1β, TNF-α, IFN-γ, or N-monomethyl-l-arginine (l-NAME) for 72 h. Parasite growth was calculated from the number of infected cells in Giemsa-stained smears. Nitric oxide production was determined with the Griess reagent. Inducible nitric oxide synthase (iNOS) expression by cardiac myocytes was detected by Western blot. The results showed that the percentages of cardiac myocytes containing T. cruzi amastigotes in cytokine-treated cultures were significantly lower than in nontreated cultures. The addition of l-NAME reversed the inhibitory effect on parasite growth of IL-1β and TNF-α but not of IFN-γ. Nitrite levels released by T. cruzi-infected and noninfected cardiac myocyte cultures after 72 h of stimulation with IL-1β were significantly higher than those produced upon treatment with TNF-α, IFN-γ, or medium alone, regardless of the infection status. Nitrite levels in TNF-α-stimulated infected cultures were significantly higher than in untreated infected cultures and TNF-α-treated noninfected cultures. l-NAME inhibited IL-1β- but not TNF-α-induced NO production, indicating the presence of iNOS-dependent and iNOS-independent mechanisms for NO formation in this experimental system. iNOS expression was detected in infected and noninfected cardiac myocytes stimulated with IL-1 β and TNF-α but not with IFN-γ. These results suggest an important role for cardiac myocytes and locally secreted cytokines in the control of parasite multiplication in T. cruzi-induced myocarditis.

Sign in / Sign up

Export Citation Format

Share Document