De Novo Design of Selective Antibiotic Peptides by Incorporation of Unnatural Amino Acids

2007 ◽  
Vol 50 (13) ◽  
pp. 3026-3036 ◽  
Author(s):  
Rickey P. Hicks ◽  
Jayendra B. Bhonsle ◽  
Divakaramenon Venugopal ◽  
Brandon W. Koser ◽  
Alan J. Magill
Keyword(s):  
Amino Acids ◽  
Unnatural Amino Acids ◽  
De Novo ◽  
De Novo Design ◽  
Antibiotic Peptides

De Novo Design of a Bolaamphiphilic Peptide with Only Natural Amino Acids

2008 ◽  
Vol 8 (11) ◽  
pp. 1053-1059 ◽  
Author(s):  
Feng Qiu ◽  
Yongzhu Chen ◽  
Chengkang Tang ◽  
Qinghan Zhou ◽  
Chen Wang ◽  
...  
Keyword(s):  
Amino Acids ◽  
De Novo ◽  
De Novo Design ◽  
Natural Amino Acids

scholarly journals Rationale-Based,De NovoDesign of Dehydrophenylalanine-Containing Antibiotic Peptides and Systematic Modification in Sequence for Enhanced Potency

2011 ◽  
Vol 55 (5) ◽  
pp. 2178-2188 ◽  
Author(s):  
Sarika Pathak ◽  
Virander Singh Chauhan
Keyword(s):  
Antimicrobial Activity ◽  
Unnatural Amino Acids ◽  
De Novo ◽  
Peptide Antibiotics ◽  
Content Type ◽  
Microbial Drug Resistance ◽  
Proteolytic Resistance ◽  
Fundamental Information ◽  
Transmission Electron ◽  
Antibiotic Peptides

ABSTRACTIncreased microbial drug resistance has generated a global requirement for new anti-infective agents. As part of an effort to develop new, low-molecular-mass peptide antibiotics, we used a rationale-based minimalist approach to design short, nonhemolytic, potent, and broad-spectrum antibiotic peptides with increased serum stability. These peptides were designed to attain an amphipathic structure in helical conformations. VS1 was used as the lead compound, and its properties were compared with three series of derivates obtained by (i) N-terminal amino acid addition, (ii) systematic Trp substitution, and (iii) peptide dendrimerization. The Trp substitution approach underlined the optimized sequence of VS2 in terms of potency, faster membrane permeation, and cost-effectiveness. VS2 (a variant of VS1 with two Trp substitutions) was found to exhibit good antimicrobial activity against both the Gram-negativeEscherichia coliand the Gram-positive bacteriumStaphylococcus aureus. It was also found to have noncytolytic activity and the ability to permeate and depolarize the bacterial membrane. Lysis of the bacterial cell wall and inner membrane by the peptide was confirmed by transmission electron microscopy. A combination of small size, the presence of unnatural amino acids, high antimicrobial activity, insignificant hemolysis, and proteolytic resistance provides fundamental information for thede novodesign of an antimicrobial peptide useful for the management of infectious disease.

De Novo Design and Synthesis of Helix–Turn–Helix Structure from Short and Robust Mixed Helices Derived from C-Linked Carbo-β-Amino Acids

2006 ◽  
Vol 45 (48) ◽  
pp. 8207-8210 ◽  
Author(s):  
Gangavaram V. M. Sharma ◽  
Velaparthi Subash ◽  
Kongari Narsimulu ◽  
Ampapathi Ravi Sankar ◽  
Ajit C. Kunwar
Keyword(s):  
Amino Acids ◽  
De Novo ◽  
De Novo Design ◽  
Design And Synthesis ◽  
Helix Structure

De Novo Design of Peptides withl-α-Nucleobase Amino Acids and Their Binding Properties to the P22 boxB RNA and Its Mutants

2004 ◽  
Vol 15 (4) ◽  
pp. 694-698 ◽  
Author(s):  
Hideo Miyanishi ◽  
Tsuyoshi Takahashi ◽  
Hisakazu Mihara
Keyword(s):  
Amino Acids ◽  
De Novo ◽  
De Novo Design ◽  
Binding Properties

De Novo Design and Synthesis of Helix–Turn–Helix Structure from Short and Robust Mixed Helices Derived from C-Linked Carbo-β-Amino Acids

2006 ◽  
Vol 118 (48) ◽  
pp. 8387-8390 ◽  
Author(s):  
Gangavaram V. M. Sharma ◽  
Velaparthi Subash ◽  
Kongari Narsimulu ◽  
Ampapathi Ravi Sankar ◽  
Ajit C. Kunwar
Keyword(s):  
Amino Acids ◽  
De Novo ◽  
De Novo Design ◽  
Design And Synthesis ◽  
Helix Structure

D-Amino acids in protein de novo design. II. Protein-diastereomerism versus protein-enantiomerism

1997 ◽  
Vol 4 (4-6) ◽  
pp. 377-386 ◽  
Author(s):  
Thomas Haack ◽  
Ma. José González ◽  
Yolanda Sánchez ◽  
Ernest Giralt
Keyword(s):  
Amino Acids ◽  
De Novo ◽  
De Novo Design ◽  
Versus Protein
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