Potential specific inhibitors of the lactose transport system of Escherichia coli

1970 ◽  
Vol 13 (4) ◽  
pp. 755-756 ◽  
Author(s):  
Emrys Wyn. Thomas
Keyword(s):  
Escherichia Coli ◽  
Transport System ◽  
Lactose Transport ◽  
Specific Inhibitors

scholarly journals 2-Deoxy-d-galactose, a substrate for the galactose-transport system of Escherichia coli

1977 ◽  
Vol 168 (1) ◽  
pp. 15-22 ◽  
Author(s):  
P J F Henderson ◽  
R A Giddens
Keyword(s):  
Escherichia Coli ◽  
Transport System ◽  
Transport Systems ◽  
Mutual Inhibition ◽  
E Coli ◽  
System 2 ◽  
Galactose Transport ◽  
Proton Uptake ◽  
Inhibition Studies ◽  
Lactose Transport

The following observations showed that 2-deoxy-D-galactose is a useful tool for the isolation and elucidation of the activity of one system for galactose uptake into Escherichia coli. 1. 2-Deoxygalactose, which is not a substrate for growth of E. coli, was transported into strains of the organism induced for galactose transport. 2. By using appropriate mutants it was shown that 2-deoxygalactose is a much better substrate for the galactose-transport system than for the methyl galactoside-transport system. This was confirmed by the results of mutual inhibition studies with substrates of each transport system. 3. The glucose-, arabinose- or lactose-transport systems did not effect significant transport of 2-deoxygalactose. 4. Like other substrates of the galactose-transport system, 2-deoxygalactose promoted effective proton uptake into de-energized suspensions of appropriate E. coli strains. 5. The S183 series of E. coli mutants were found to contain a constitutive galactose-transport system, if 2-deoxygalactose transport is used as one criterion for such activity.

scholarly journals Energy Coupling in the Lactose Transport System of Escherichia coli

1970 ◽  
Vol 65 (1) ◽  
pp. 63-69 ◽  
Author(s):  
P. T. S. Wong ◽  
E. R. Kashket ◽  
T. H. Wilson
Keyword(s):  
Escherichia Coli ◽  
Transport System ◽  
Energy Coupling ◽  
Lactose Transport
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