Irreversible enzyme inhibitors. 184. Affinity column for purification of rat liver guanine deaminase and xanthine oxidase

1971 ◽  
Vol 14 (9) ◽  
pp. 799-801 ◽  
Author(s):  
B. R. Baker ◽  
Hans U. Siebeneick
Keyword(s):  
Xanthine Oxidase ◽  
Rat Liver ◽  
Enzyme Inhibitors ◽  
Affinity Column ◽  
Guanine Deaminase

Novel C-9, 9'-O-acyl Esters of (-)-Carinol as Free-radical Scavengers and Xanthine Oxidase Enzyme Inhibitors: Synthesis and Biological Evaluation

2013 ◽  
Vol 9 (1) ◽  
pp. 100-103 ◽  
Author(s):  
Praveen Kumar Suryadevara ◽  
Hari Babu Tatipaka ◽  
Rama Subba Rao Vidadala ◽  
Ashok k Tiwari ◽  
Janaswamy Madhusudana Rao ◽  
...  
Keyword(s):  
Free Radical ◽  
Xanthine Oxidase ◽  
Enzyme Inhibitors ◽  
Biological Evaluation ◽  
Free Radical Scavengers ◽  
Radical Scavengers ◽  
Oxidase Enzyme ◽  
Synthesis And Biological Evaluation

Development of Guanase and Xanthine Oxidase in Rat Liver

1969 ◽  
Vol 132 (1) ◽  
pp. 91-92 ◽  
Author(s):  
E. Silverstein ◽  
O. Zaklynsky ◽  
T. Horn
Keyword(s):  
Xanthine Oxidase ◽  
Rat Liver

Mechanisms of conversion of xanthine dehydrogenase to xanthine oxidase in ischemic rat liver and kidney

1988 ◽  
Vol 254 (5) ◽  
pp. G753-G760 ◽  
Author(s):  
T. G. McKelvey ◽  
M. E. Hollwarth ◽  
D. N. Granger ◽  
T. D. Engerson ◽  
U. Landler ◽  
...  
Keyword(s):  
Xanthine Oxidase ◽  
Rat Liver ◽  
Ischemia Reperfusion ◽  
Xanthine Dehydrogenase ◽  
Serum Glutamic Oxaloacetic Transaminase ◽  
Liver Model ◽  
Damage Process ◽  
Liver And Kidney ◽  
Sulfhydryl Modification

Previous studies have proposed and supported a role for the proteolytic, irreversible conversion of xanthine dehydrogenase to xanthine oxidase (XO) in postischemic injury in a wide variety of organs. A second mechanism of conversion, due to sulfhydryl modification and reversible with dithiothreitol (DTT), is potentially important but has not been well investigated. In this study rat liver and kidney were found to produce significant amounts of DTT-reversible XO during normothermic global ischemia. Formation of reversible XO precedes that of irreversible XO by approximately 0.5 h with a strong correlation (r = 0.92) existing between the rate of irreversible XO formation and the concentration of reversible XO. The formation of reversible XO is preceded by a depletion of glutathione with concentrations of glutathione during ischemia correlating (r = 0.85) with the observed concentration of reversible XO. While a large increase in the extent of liver damage occurs concurrently with conversion in an in vivo liver model of liver ischemia, an ischemia-reperfusion regimen (1 h of ischemia plus 0.5 h of reperfusion) that resulted in no conversion caused significant elevations in serum glutamic pyruvic transaminase and serum glutamic-oxaloacetic transaminase. Rats depleted of XO by tungsten dieting release 65% less enzyme after the same insult, suggesting that endogenous XO may also participate in the damage process independent of any conversion.

scholarly journals METABOLIC ACTIVITY OF INOSINE ON THE INCORPORATION OF D-RIBOFLAVIN INTO RAT-LIVER XANTHINE OXIDASE SYSTEM

1965 ◽  
Vol 11 (4) ◽  
pp. 271-274 ◽  
Author(s):  
TATSUYUKI SUGAHARA ◽  
TAKAO SUZUKI ◽  
YOSHIKAZU SAHASHI
Keyword(s):  
Xanthine Oxidase ◽  
Rat Liver ◽  
Metabolic Activity ◽  
Oxidase System
Sign in / Sign up

Export Citation Format

Share Document