Separation and Purification of Anthocyanins by High-Speed Countercurrent Chromatography and Screening for Antioxidant Activity

2000 ◽  
Vol 48 (2) ◽  
pp. 338-343 ◽  
Author(s):  
Andreas Degenhardt ◽  
Holger Knapp ◽  
Peter Winterhalter
Keyword(s):  
Antioxidant Activity ◽  
High Speed ◽  
Countercurrent Chromatography ◽  
Separation And Purification

Separation and purification of 9,10-dihydrophenanthrenes and bibenzyls fromPholidota chinensisby high-speed countercurrent chromatography

2015 ◽  
Vol 38 (3) ◽  
pp. 453-459 ◽  
Author(s):  
Yang Chen ◽  
Shining Cai ◽  
Liang Deng ◽  
Qiang Xia ◽  
Lian-Feng Du ◽  
...  
Keyword(s):  
High Speed ◽  
Countercurrent Chromatography ◽  
Separation And Purification

scholarly journals Isolation and Identification of Anthocyanin Component in the Fruits of Acanthopanax Sessiliflorus (Rupr. & Maxim.) Seem. by Means of High Speed Counter Current Chromatography and Evaluation of Its Antioxidant Activity

Molecules ◽  
2020 ◽  
Vol 25 (8) ◽  
pp. 1781 ◽  
Author(s):  
Liang Chen ◽  
Xiulan Xin ◽  
Hui Feng ◽  
Shuangshi Li ◽  
Qiguang Cao ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
High Speed ◽  
Radical Scavenging ◽  
Ionization Mass ◽  
Anthocyanin Content ◽  
Separation And Purification ◽  
Isolation And Identification ◽  
Antioxidant Power ◽  
Acanthopanax Sessiliflorus ◽  
Counter Current

Acanthopanax sessiliflorus (Rupr. & Maxim.) Seem. (Araliaceae) is one of the most abundant species of genus Acanthopanax. The fruits of A. sessiliflorus are used in traditional medical protocols as an analgesic, tonic, antidiabetic, antihypertensive, anti-inflammatory, antitumor, and immune-stimulating agent. In this work, we carried out a comprehensive investigation into the anthocyanin components in the fruits of A. sessiliflorus. The anthocyanin content in the fresh fruits of A. sessiliflorus was determined by high performance liquid chromatography-diode array detection (HPLC/DAD), and the anthocyanin component was isolated from these using high-speed counter-current chromatography (HSCCC) and elucidated by electro-spray ionization-mass spectrometry (ESI/MS), 1H- and 13C-NMR. Its antioxidant activity was evaluated by ferric-reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH). We found that A. sessiliflorus contained a gross anthocyanin content of 121.35 mg/100 g. HSCCC was successfully used for separation and purification of the primary anthocyanin component, cyanidin 3-xylosyl-galactoside. The antioxidant and radical scavenging tests indicated that cyanidin 3-xylosyl-galactoside is a potent antioxidant.

Simulated Digestion and Antioxidant Activity of Red Wine Fractions Separated by High Speed Countercurrent Chromatography

2008 ◽  
Vol 56 (19) ◽  
pp. 8879-8884 ◽  
Author(s):  
Maria Noguer ◽  
Ana B. Cerezo ◽  
Michael Rentzsch ◽  
Peter Winterhalter ◽  
Ana M. Troncoso ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
High Speed ◽  
Red Wine ◽  
Countercurrent Chromatography ◽  
Simulated Digestion

scholarly journals A fast and efficient preparative method for separation and purification of main bioactive xanthones from the waste of Garcinia mangostana L. by high-speed countercurrent chromatography

2021 ◽  
pp. 103252
Author(s):  
Ricardo Felipe Alexandre de Mello ◽  
Wandson B. de Souza Pinheiro ◽  
Jaisielle Kelem F. Benjamim ◽  
Francilia Campos de Siqueira ◽  
Renan Campos Chisté ◽  
...  
Keyword(s):  
High Speed ◽  
Preparative Method ◽  
Countercurrent Chromatography ◽  
Separation And Purification ◽  
Garcinia Mangostana
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