Low-Density Lipoprotein Antioxidant Activity of Phenolic Compounds and Polyphenol Oxidase Activity in Selected Clingstone Peach Cultivars

2000 ◽  
Vol 48 (2) ◽  
pp. 147-151 ◽  
Author(s):  
Susan Chang ◽  
Christine Tan ◽  
Edwin N. Frankel ◽  
Diane M. Barrett
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Polyphenol Oxidase ◽  
Oxidase Activity ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Low Density ◽  
Polyphenol Oxidase Activity

4.P.168 Low density lipoprotein-bound isoflavonoid genistein: Structure-antioxidant activity relationships

1997 ◽  
Vol 134 (1-2) ◽  
pp. 331
Author(s):  
Q.H. Meng ◽  
H. Adlercreutz ◽  
K. Wähälä ◽  
M.J. Tikkanen
Keyword(s):  
Antioxidant Activity ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Low Density

scholarly journals Spectrophotometric and Smartphone-Assisted Determination of Phenolic Compounds Using Crude Eggplant Extract

Molecules ◽  
2019 ◽  
Vol 24 (23) ◽  
pp. 4407 ◽  
Author(s):  
M.A. Morosanova ◽  
A.S. Bashkatova ◽  
E.I. Morosanova
Keyword(s):  
Phenolic Compounds ◽  
Chlorogenic Acid ◽  
Caffeic Acid ◽  
Polyphenol Oxidase ◽  
Oxidase Activity ◽  
Biological Fluids ◽  
Low Cost ◽  
Analytical Signal ◽  
Polyphenol Oxidase Activity

In order to develop a simple, reliable and low cost enzymatic method for the determination of phenolic compounds we studied polyphenol oxidase activity of crude eggplant (S. melongena) extract using 13 phenolic compounds. Catechol, caffeic and chlorogenic acids, and L-DOPA have been rapidly oxidized with the formation of colored products. Monophenolic compounds have been oxidized at a much slower speed. Ferulic acid, quercetin, rutin, and dihydroquercetin have been found to inhibit polyphenol oxidase activity of crude eggplant extract. The influence of pH, temperature, crude eggplant extract amount, and 3-methyl-2-benzothiazolinone hydrazone (MBTH) concentration on the oxidation of catechol, caffeic acid, chlorogenic acid, and L-DOPA has been investigated spectrophotometrically. Michaelis constants values decrease by a factor of 2 to 3 in the presence of MBTH. Spectrophotometric (cuvette and microplate variants) and smartphone-assisted procedures for phenolic compounds determination have been proposed. Average saturation values (HSV color model) of the images of the microplate wells have been chosen as the analytical signal for smartphone-assisted procedure. LOD values for catechol, caffeic acid, chlorogenic acid, and L-DOPA equaled 5.1, 6.3, 5.8 and 30.0 µM (cuvette procedure), 12.2, 13.2, 13.2 and 80.4 µM (microplate procedure), and 23.5, 26.4, 20.8 and 120.6 µM (smartphone procedure). All the variants have been successfully applied for fast (4-5 min) and simple TPC determination in plant derived products and L-DOPA determination in model biological fluids. The values found with smartphone procedure are in good agreement with both spectrophotometric procedures values and reference values. Using crude eggplant extract- mediated reactions combined with smartphone camera detection has allowed creating low-cost, reliable and environmentally friendly analytical method for the determination of phenolic compounds.

scholarly journals Ex VivoAntioxidant Activity of Selected Medicinal Plants against Fenton Reaction-Mediated Oxidation of Biological Lipid Substrates

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Namratha Pai Kotebagilu ◽  
Vanitha Reddy Palvai ◽  
Asna Urooj
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Fenton Reaction ◽  
Methanol Extract ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Andrographis Paniculata ◽  
Antioxidant Potential ◽  
Low Density ◽  
Mediated Oxidation

Free radical-mediated oxidation is often linked to various degenerative diseases. Biological substrates with lipids as major components are susceptible to oxygen-derived lipid peroxidation due to their composition. Lipid peroxide products act as biomarkers in evaluating the antioxidant potential of various plants and functional foods. The study focused on evaluation of the antioxidant potential of two extracts (methanol and 80% methanol) of four medicinal plants,Andrographis paniculata,Costus speciosus, Canthium parviflorum, andAbrus precatorius, against Fenton reaction-mediated oxidation of three biological lipid substrates; cholesterol, low-density lipoprotein, and brain homogenate. The antioxidant activity of the extracts was measured by thiobarbituric acid reactive substances method. Also, the correlation between the polyphenol, flavonoid content, and the antioxidant activity in biological substrates was analyzed. Results indicated highest antioxidant potential by 80% methanol extract ofCanthium parviflorum(97.55%), methanol extract ofAndrographis paniculata(72.15%), and methanol extract ofCanthium parviflorum(49.55%) in cholesterol, low-density lipoprotein, and brain, respectively. The polyphenol and flavonoid contents of methanol extract ofAndrographis paniculatain cholesterol (r=0.816) and low-density lipoprotein (r=0.948) andCostus speciosusin brain (r=0.977, polyphenols, andr=0.949, flavonoids) correlated well with the antioxidant activity. The findings prove the antioxidant potential of the selected medicinal plants against Fenton reaction in biological lipid substrates.

Protective effect of olive oil and its phenolic compounds against low density lipoprotein oxidation

Lipids ◽  
2000 ◽  
Vol 35 (6) ◽  
pp. 633-638 ◽  
Author(s):  
Montserrat Fitó ◽  
María Isabel Covas ◽  
Rosa M. Lamuela-Raventós ◽  
Joan Vila ◽  
Jaume Torrents ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Protective Effect ◽  
Olive Oil ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Low Density ◽  
Lipoprotein Oxidation ◽  
Low Density Lipoprotein Oxidation

scholarly journals Evaluation of Antihyperlipidaemic and Antioxidant Activity of Astercantha longifolia (Linn.) Nees and Pergularia daemia (Forsskal) Chiov

2020 ◽  
pp. 134-138
Author(s):  
T. P. Kumari Pushpa Rani ◽  
A. Doss ◽  
S. P. Anand
Keyword(s):  
Antioxidant Activity ◽  
Biochemical Parameters ◽  
Methanol Extract ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Lipoprotein Cholesterol ◽  
Low Density ◽  
Low Density Lipoprotein Cholesterol

Objective: To evaluate the antioxidant and antihyperlipidaemic effect of methanol extracts of Asteracantha longifolia and Pergularia daemia leaf in alloxan induced diabetic rats. Methods: Swiss albino rats were made diabetic by a single dose of alloxan monohydrate (150 mg/kg i.p.). Blood glucose levels and body weights of rats were measured using on weekly intervals i.e day 0,7,14 and 21 after daily administration of both extracts at dose 200 mg/kg bw. Other biochemical parameters such as serum cholesterol, triglycerides, low density lipoprotein-cholesterol, very low density lipoprotein-cholesterol, high density lipoprotein-cholesterol, phospholipids and serum protein, albumin, globulin levels were also measured at the end of study. The antioxidant enzymes (CAT, SOD & GSH) were also measured in the diabetic rats. Results: In the acute toxicity study, methanol extract of both plants were non-toxic at 2000 mg/kg in rats. The increased body weight, decreased blood glucose and other biochemical parameters level were observed in diabetic rats treated with methanol extracts of both plants compared to diabetic control rats. The antioxidant activity of both plant extracts were also exhibited significant activity. In diabetic rats, methanol extract of both plants administration, altered lipid profiles were reversed to near normal than diabetic control rats. Conclusions: Methanol extract of both plants (Asteracantha longifolia and Pergularia daemia) leaf possesses significant antioxidant and antihyperlipidaemic activity in diabetic rats.

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