An in Vitro Study of Wheat Bran Binding Capacity for Hg, Cd, and Pb

1999 ◽  
Vol 47 (11) ◽  
pp. 4714-4717 ◽  
Author(s):  
Shiyi Ou ◽  
Kongrong Gao ◽  
Yan Li
1995 ◽  
Vol 74 (2) ◽  
pp. 221-228 ◽  
Author(s):  
Ingrid C. Gelissen ◽  
Martin A. Eastwood

The association of radiolabelled taurocholic acid with the solid fraction of a faecal fermentation mixture was measured. A human faecal inoculum was incubated with [24-14C]taurocholic acid and several non-starch polysaccharide sources (pectin, wheat bran, ispaghula (Plantago ovata) husk and seed), glucose or a substrate-free control. Portions of fermentation mixture were taken at 0, 3, 6, 21 and 24 h and centrifuged to acquire a supernatant fraction and a pellet containing the fermentation residue. 14C was measured in supernatant fractions and pellets at all time points. Volatile fatty acids (VFA) were measured at 0 and 24 h to confirm bacterial growth. Radioactivity in the pellet increased over time for all substrates. Glucose resulted in the greatest incorporation of taurocholic acid into the pellet, followed by pectin. At 24 h the proportion of the total radioactivity found in the pellet was 92% for glucose, 79% for pectin, 60% for wheat bran, 59% for ispaghula seed, 53% for ispaghula husk and 26% for the control (mean of duplicates). Glucose and pectin produced the greatest quantity of VFA at 24 h. VFA production was highly correlated with radioactivity in the pellet (r 0·976, P <0·005). These results suggest that the bile acid binding capacity of a faecal culture mixture may be strongly influenced by the fermentability of the available substrate and hence related to bacterial metabolic activity.


Nephrology ◽  
2018 ◽  
Vol 24 (2) ◽  
pp. 221-226 ◽  
Author(s):  
Stefan P Schumacher ◽  
Leon J Schurgers ◽  
Marc G Vervloet ◽  
Aegida Neradova

1973 ◽  
Vol 19 (6) ◽  
pp. 501-504
Author(s):  
Shigeru ONO ◽  
Hiroko HIRANO ◽  
Kijuro OBARA

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