Identification of Sole(Solea solea)and Greenland Halibut(Reinhardtius hippoglossoides)by PCR Amplification of the 5S rDNA Gene

1999 ◽  
Vol 47 (3) ◽  
pp. 1046-1050 ◽  
Author(s):  
Ana Céspedes ◽  
Teresa García ◽  
Esther Carrera ◽  
Isabel González ◽  
Alicia Fernández ◽  
...  
Keyword(s):  
Pcr Amplification ◽  
5S Rdna ◽  
Greenland Halibut ◽  
Rdna Gene ◽  
Solea Solea ◽  
Greenland Halibut Reinhardtius Hippoglossoides ◽  
Reinhardtius Hippoglossoides

Indirect Enzyme-Linked Immunosorbent Assay for the Identification of Sole (Solea solea), European Plaice (Pleuronectes platessa), Flounder (Platichthys flesus), and Greenland Halibut (Reinhardtius hippoglossoides)

1999 ◽  
Vol 62 (10) ◽  
pp. 1178-1182 ◽  
Author(s):  
ANA CÉSPEDES ◽  
TERESA GARCÍA ◽  
ESTHER CARRERA ◽  
ISABEL GONZÁLEZ ◽  
ALICIA FERNÁNDEZ ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Greenland Halibut ◽  
Flatfish Species ◽  
Pleuronectes Platessa ◽  
Platichthys Flesus ◽  
Solea Solea ◽  
Fish Samples ◽  
Greenland Halibut Reinhardtius Hippoglossoides ◽  
Reinhardtius Hippoglossoides

Polyclonal antibodies produced against soluble muscle protein extracts from sole (Solea solea), European plaice (Pleuronectes platessa), flounder (Platichthys flesus), and Greenland halibut (Reinhardtius hippoglossoides) were used in an indirect enzyme-linked immunosorbent assay for the specific identification of fillets from these flatfish species. The assay was performed in two different formats: microtiter plates and immunostick tubes. Immunorecognition of antibodies adsorbed to their specific fish samples was made with goat antirabbit immunoglobulins conjugated to the enzyme horseradish peroxidase. Subsequent enzymatic conversion of the substrate allowed unequivocal identification of all flatfish species studied.

scholarly journals Reproductive strategy, egg characteristics and embryonic development of Greenland halibut (Reinhardtius hippoglossoides)

2012 ◽  
Vol 70 (2) ◽  
pp. 342-351 ◽  
Author(s):  
Rosario Domínguez-Petit ◽  
Patrick Ouellet ◽  
Yvan Lambert
Keyword(s):  
Embryonic Development ◽  
Reproductive Strategy ◽  
Biochemical Composition ◽  
Incubation Temperature ◽  
Life Stage ◽  
Early Life Stage ◽  
Greenland Halibut ◽  
Hatching Time ◽  
Greenland Halibut Reinhardtius Hippoglossoides ◽  
Reinhardtius Hippoglossoides

Abstract Domínguez-Petit, R., Ouellet, P., and Lambert, Y. 2013. Reproductive strategy, egg characteristics and embryonic development of Greenland halibut (Reinhardtius hippoglossoides). – ICES Journal of Marine Science, 70: 342–351. Despite the commercial importance of Greenland halibut (GH), important gaps exist in our knowledge of the reproductive and early life stage biology for this species. The present study examined through laboratory experiments the spawning strategy, realized fecundity, egg characteristics, biochemical composition, and embryonic development of GH. The results confirmed the hypothesis that GH is a single-batch spawner producing large eggs, resulting in low realized fecundity. Embryonic development and hatching time are highly dependent on incubation temperature; 50% hatching occurred after 46, 30, and 24 days at 2, 4, and 6°C, respectively. Few changes in the biochemical composition of the eggs are observed during embryonic development. Newly hatched larvae are not well developed, having a large yolk sac, no pigmentation and incomplete development of the jaws. Egg specific density confirmed the mesopelagic distribution of the eggs at sea. However, important buoyancy changes occurring in the last 3–4 days before hatching indicate that larvae hatch higher in the water column. These results are important for understanding advection and dispersion processes of GH eggs and larvae and the connectivity between spawning grounds and nursery areas.

Use of Parasites for Separating Stocks of Greenland Halibut (Reinhardtius hippoglossoides) in the Canadian Northwest Atlantic

1993 ◽  
Vol 50 (10) ◽  
pp. 2175-2181 ◽  
Author(s):  
J. R. Arthur ◽  
E. Albert
Keyword(s):  
Atlantic Coast ◽  
Large Fish ◽  
Adult Fish ◽  
Greenland Halibut ◽  
Pseudoterranova Decipiens ◽  
Saguenay Fjord ◽  
Greenland Halibut Reinhardtius Hippoglossoides ◽  
Reinhardtius Hippoglossoides ◽  
Biological Tags ◽  
Discriminant Function Analyses

The usefulness of parasites as biological tags for stocks of Greenland halibut (Reinharditius hippoglossoides) occurring off the Atlantic coast of Canada and in the Gulf of St. Lawrence was investigated. Nonparametric discriminant function analyses of eight collections comprising a total of 231 large fish (>39 cm) using counts for selected parasite taxa (Corynosoma strumosum juvenile, Otodistomum sp. metacercaria, Contracaecinea spp. larva, Anisakis simplex larva, and Pseudoterranova decipiens larva) gave accurate results (almost 100% correct classification) for the separation of fish from the Gulf of St. Lawrence from those collected from adjacent areas of the Saguenay Fjord and the Atlantic Ocean off Labrador. Little, if any, mixing of subadult or adult fish apparently occurs among these areas.

scholarly journals Chromosome-level assembly reveals a putative Y-autosomal fusion in the sex determination system of the Greenland Halibut (Reinhardtius hippoglossoides)

2021 ◽  
Author(s):  
Anne-Laure Ferchaud ◽  
Claire Mérot ◽  
Eric Normandeau ◽  
Jiannis Ragoussis ◽  
Charles Babin ◽  
...  
Keyword(s):  
Sex Determination ◽  
Single Molecule ◽  
Sex Chromosome ◽  
Whole Genome Sequence ◽  
Greenland Halibut ◽  
Syntenic Relationship ◽  
Greenland Halibut Reinhardtius Hippoglossoides ◽  
Reinhardtius Hippoglossoides ◽  
Sex Determination System ◽  
Chromosome Level

Abstract Despite the commercial importance of Greenland Halibut (Reinhardtius hippoglossoides), important gaps still persist in our knowledge of this species, including its reproductive biology and sex determination mechanism. Here, we combined single-molecule sequencing of long reads (Pacific Sciences) with chromatin conformation capture sequencing (Hi-C) data to assemble the first chromosome-level reference genome for this species. The high-quality assembly encompassed more than 598 Megabases (Mb) assigned to 1 594 scaffolds (scaffold N50 = 25 Mb) with 96% of its total length distributed among 24 chromosomes. Investigation of the syntenic relationship with other economically important flatfish species revealed a high conservation of synteny blocks among members of this phylogenetic clade. Sex determination analysis revealed that, similar to other teleost fishes, flatfishes also exhibit a high level of plasticity and turnover in sex-determination mechanisms. A low-coverage whole-genome sequence analysis of 198 individuals revealed that Greenland Halibut possesses a male heterogametic XY system and several putative candidate genes implied in the sex determination of this species. Our study also suggests for the first time in flatfishes that a putative Y-autosomal fusion could be associated with a reduction of recombination typical of the early steps of sex chromosome evolution.

Evidence supporting panmixia in Greenland halibut (Reinhardtius hippoglossoides) in the Northwest Atlantic

2014 ◽  
Vol 71 (5) ◽  
pp. 763-774 ◽  
Author(s):  
Denis Roy ◽  
David C. Hardie ◽  
Margaret A. Treble ◽  
James D. Reist ◽  
Daniel E. Ruzzante
Keyword(s):  
Population Structure ◽  
Population Genetic ◽  
Conservation Strategies ◽  
Greenland Halibut ◽  
Northwest Atlantic ◽  
Population Genetic Differentiation ◽  
Conservation Implications ◽  
Greenland Halibut Reinhardtius Hippoglossoides ◽  
Reinhardtius Hippoglossoides ◽  
Species Specific

Assessment of population structure is critical to the design and implementation of sound management and conservation strategies. However, population structure must be assessed using markers attuned to population genetic processes such as genetic drift and gene flow, which reflect actual levels of reproductive isolation among putative genetic clusters. This is critical for highly exploited, commercial species that form the backbone of regional and local economies. Here, we show extremely low levels of population genetic differentiation among Greenland halibut (Reinhardtius hippoglossoides) collected from throughout the Northwest Atlantic, which cannot be statistically differentiated from panmixia using 12 species-specific polymorphic microsatellite markers. In contrast, some previous studies have demonstrated significant differences among individuals collected from various parts of the species’ range using a variety of both genetic and nongenetic techniques. In accordance with other reports and consistent with the species’ life history, we demonstrate that the most parsimonious explanation reconciling observed patterns is a repeated high degree of local differentiation of new recruits and colonizers originating from a common gene pool. Such a scenario has important conservation implications in terms of devising more appropriate strategies balancing species persistence and replenishment with sustainable resource use.

Sign in / Sign up

Export Citation Format

Share Document