Characterization of Residual Lignin after SO2-Catalyzed Steam Explosion and Enzymatic Hydrolysis ofEucalyptus viminalisWood Chips

1999 ◽  
Vol 47 (6) ◽  
pp. 2295-2302 ◽  
Author(s):  
L. P. Ramos ◽  
A. L. Mathias ◽  
F. T. Silva ◽  
A. R. Cotrim ◽  
A. L. Ferraz ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Steam Explosion ◽  
Residual Lignin

Characterization of autohydrolysis aspen (P. tremuloides) lignins. Part 4. Residual autohydrolysis lignin

1979 ◽  
Vol 57 (19) ◽  
pp. 2612-2616 ◽  
Author(s):  
Morris Wayman ◽  
Miranda G. S. Chua
Keyword(s):  
Enzymatic Hydrolysis ◽  
Time Varying ◽  
Residual Lignin ◽  
Aspen Wood ◽  
Milled Wood Lignin ◽  
Wood Meal ◽  
Nitrobenzene Oxidation ◽  
Infrared Studies ◽  
Lignocellulosic Residue

Lignocellulosic residue remaining after autohydrolysis of extractive-free aspen wood meal at 195 °C for periods of time varying from 5 to 120 min followed by extraction with 90% dioxane was subjected to enzymatic hydrolysis to obtain residual lignin. Infrared studies indicated that in the early stages of autohydrolysis residual lignin resembles protolignin, but as autohydrolysis proceeds it changes to resemble more and more the extracted lignin. Residual lignin was found to be higher in carbon but lower in hydrogen and oxygen than aspen milled wood lignin. The methoxyl content was also lower than the reference lignin. From alkaline nitrobenzene oxidation, residual lignin is seen to become more condensed with increasing autohydrolysis time. The insolubility of residual lignin is attributed to the existence of strong bonds between this lignin and carbohydrate.

scholarly journals Characterization of Residual Lignin Obtained by the Enzymatic Hydrolysis of Oil Palm Empty Fruit Bunch Pulps

BioResources ◽  
2016 ◽  
Vol 12 (1) ◽  
Author(s):  
Yin Ying H'ng ◽  
Akiko Nakagawa-Izumi ◽  
Cheu Peng Leh ◽  
Atanu Kumar Das ◽  
Hiroshi Ohi
Keyword(s):  
Enzymatic Hydrolysis ◽  
Oil Palm ◽  
Empty Fruit Bunch ◽  
Residual Lignin ◽  
Hydrolysis Of

Multifaceted characterization of sugarcane bagasse under different steam explosion severity conditions leading to distinct enzymatic hydrolysis yields

2019 ◽  
Vol 139 ◽  
pp. 111542 ◽  
Author(s):  
Melissa Cristina do Espírito Santo ◽  
Eliano Brito Cardoso ◽  
Francisco Eduardo Gontijo Guimaraes ◽  
Eduardo Ribeiro deAzevedo ◽  
Giovanni Paro da Cunha ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Sugarcane Bagasse ◽  
Steam Explosion

scholarly journals Effects on Lignin Redistribution in Eucalyptus globulus Fibres Pre-Treated by Steam Explosion: A Microscale Study to Cellulose Accessibility

Biomolecules ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 507
Author(s):  
Eduardo Troncoso-Ortega ◽  
Rosario del P. Castillo ◽  
Pablo Reyes-Contreras ◽  
Patricia Castaño-Rivera ◽  
Regis Teixeira Mendonça ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Eucalyptus Globulus ◽  
Steam Explosion ◽  
Laser Scanning ◽  
Structural Changes ◽  
Micro Particles ◽  
Cellulose Accessibility ◽  
Ft Ir ◽  
Enzymatic Accessibility ◽  
Physical And Chemical

The objective of this study was to investigate structural changes and lignin redistribution in Eucalyptus globulus pre-treated by steam explosion under different degrees of severity (S0), in order to evaluate their effect on cellulose accessibility by enzymatic hydrolysis. Approximately 87.7% to 98.5% of original glucans were retained in the pre-treated material. Glucose yields after the enzymatic hydrolysis of pre-treated material improved from 19.4% to 85.1% when S0 was increased from 8.53 to 10.42. One of the main reasons for the increase in glucose yield was the redistribution of lignin as micro-particles were deposited on the surface and interior of the fibre cell wall. This information was confirmed by laser scanning confocal fluorescence and FT-IR imaging; these microscopic techniques show changes in the physical and chemical characteristics of pre-treated fibres. In addition, the results allowed the construction of an explanatory model for microscale understanding of the enzymatic accessibility mechanism in the pre-treated lignocellulose.

Characterization of Physical, Mechanical, and Biological Properties of SilkBridge Nerve Conduit after Enzymatic Hydrolysis

2020 ◽  
Vol 3 (12) ◽  
pp. 8361-8374
Author(s):  
Silvia Biggi ◽  
Giulia A. Bassani ◽  
Valentina Vincoli ◽  
Daniele Peroni ◽  
Valerio Bonaldo ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Biological Properties ◽  
Nerve Conduit

scholarly journals Understanding the effects of different residual lignin fractions in acid-pretreated bamboo residues on its enzymatic digestibility

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Wenqian Lin ◽  
Jinlai Yang ◽  
Yayue Zheng ◽  
Caoxing Huang ◽  
Qiang Yong
Keyword(s):  
Enzymatic Hydrolysis ◽  
Negative Impact ◽  
Dilute Acid Pretreatment ◽  
Residual Lignin ◽  
Dilute Acid ◽  
Enzymatic Digestibility ◽  
Acid Pretreatment ◽  
Organic Reagents ◽  
Milled Wood Lignin ◽  
Affinity Constants

Abstract Background During the dilute acid pretreatment process, the resulting pseudo-lignin and lignin droplets deposited on the surface of lignocellulose and inhibit the enzymatic digestibility of cellulose in lignocellulose. However, how these lignins interact with cellulase enzymes and then affect enzymatic hydrolysis is still unknown. In this work, different fractions of surface lignin (SL) obtained from dilute acid-pretreated bamboo residues (DAP-BR) were extracted by various organic reagents and the residual lignin in extracted DAP-BR was obtained by the milled wood lignin (MWL) method. All of the lignin fractions obtained from DAP-BR were used to investigate the mechanism for interaction between lignin and cellulase using surface plasmon resonance (SPR) technology to understand how they affect enzymatic hydrolysis Results The results showed that removing surface lignin significantly decreased the yield for enzymatic hydrolysis DAP-BR from 36.5% to 18.6%. The addition of MWL samples to Avicel inhibited its enzymatic hydrolysis, while different SL samples showed slight increases in enzymatic digestibility. Due to the higher molecular weight and hydrophobicity of MWL samples versus SL samples, a stronger affinity for MWL (KD = 6.8–24.7 nM) was found versus that of SL (KD = 39.4–52.6 nM) by SPR analysis. The affinity constants of all tested lignins exhibited good correlations (r > 0.6) with the effects on enzymatic digestibility of extracted DAP-BR and Avicel. Conclusions This work revealed that the surface lignin on DAP-BR is necessary for maintaining enzyme digestibility levels, and its removal has a negative impact on substrate digestibility.

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