Total Antioxidant Capacity of Teas by the Ferric Reducing/Antioxidant Power Assay

1999 ◽  
Vol 47 (2) ◽  
pp. 633-636 ◽  
Author(s):  
Iris F. F. Benzie ◽  
Y. T. Szeto
Keyword(s):  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Antioxidant Power ◽  
Total Antioxidant ◽  
Ferric Reducing Antioxidant Power

Total Antioxidant Capacity with Peak Specificity via Reaction Flow Chromatography and the Ferric Reducing Antioxidant Power Assay

2019 ◽  
Vol 13 (3) ◽  
pp. 608-616 ◽  
Author(s):  
Andrew Jones ◽  
Agustín Acquaviva ◽  
Thirada Suktham ◽  
Gary R. Dennis ◽  
R. Andrew Shalliker ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Antioxidant Power ◽  
Total Antioxidant ◽  
Ferric Reducing Antioxidant Power

scholarly journals Chemical Characterization and Antioxidant Activity of Cedrus atlantica Manetti Tar (Atlas Cedar Tar)

2020 ◽  
Vol 8 (2) ◽  
pp. 244-255
Author(s):  
Inssaf Skanderi ◽  
Ourida Chouitah
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Condensed Tannins ◽  
Chemical Characterization ◽  
Antioxidant Power ◽  
Cedrus Atlantica ◽  
Total Antioxidant ◽  
Ferric Reducing Antioxidant Power ◽  
Total Polyphenolic Content

Cedrus atlantica Manetti Tar was analyzed by gas chromatography coupled to mass spectrometry, it was subjected to analyzes to know their total polyphenolic and condensed tannins contents, also their Ferric-reducing antioxidant power and Total antioxidant capacity. Chemical characterization identified 88 constituents where Himachalene and α-atlantone isomers (14.51 % - 4.07 %), Calacorene (3.52 %) and ar-Turmerone 3.35 %, were the major components, the total polyphenolic content and condensed tannins contents were 57.15 ± 0.15 milligrams equivalent of gallic acid /g tar and 4.41 ± 0.05 milligrams equivalent of catechin /g tar respectively .This extract showed remarkable Ferric-reducing antioxidant power with effective concentration equal to 50 ± 0.075 mg /mL ± 0,00028 and total antioxidant capacity equal to 262.75 mg equivalents of ascorbic acid /g tar ± 14,43. The experimental results indicated that our tar has promotive antioxidant activity.

Total Antioxidant Capacity of Thai Herbal Teas by the Ferric Reducing Antioxidant Power

2019 ◽  
Vol 886 ◽  
pp. 46-51
Author(s):  
Techaoei Surachai ◽  
Jarmkom Khemjira ◽  
Khobjai Warachate
Keyword(s):  
Vitamin C ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Herbal Tea ◽  
Frap Assay ◽  
Antioxidant Power ◽  
Intermediate Activity ◽  
Total Antioxidant ◽  
Ferric Reducing Antioxidant Power

The main objective aimed to compare in vitro antioxidant power of different recipes of Thai herbal teas including of Tatirot, Krajeab, Kamfoi, and Kesorn Bua. The ferric reducing/antioxidant power (FRAP) assay was used to measure the total antioxidant power of freshly brewed tea. Results showed that different Thai tea recipes had slightly different in vitro antioxidant power. The herbal teas recipe was expressed as µM of antioxidant power/g of dried Thai tea recipes. Values ranges as 555.62±0.77-908.43±0.69 µM/1g of Thai herbal tea, especially Krajeab tea showed strongly antioxidant of 908.43±0.69 µM/1g of tea when compared with other samples. Therefore, it has confirmed that the antioxidant power of Thai herbal tea recipes is considerably intermediate activity than vitamin C

scholarly journals Comparison of total antioxidant capacity of saliva in men based on daily cigarette consumption

2020 ◽  
Vol 24 (1) ◽  
Author(s):  
Pourya Pashaei ◽  
Zahra Alizadeh ◽  
Hossein Piri ◽  
Monirsadat Mirzadeh ◽  
Fatemeh Kazemi
Keyword(s):  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Cross Sectional Study ◽  
Normal Male ◽  
Cross Sectional ◽  
Antioxidant Power ◽  
Significant Difference ◽  
Total Antioxidant ◽  
Ferric Reducing Antioxidant Power ◽  
Nitrogen Radicals

Objective: Antioxidants play an important role in inhibiting free oxygen and nitrogen radicals and preventing their formation. There are contradictory results regarding the relationship between cigarette smoking and total antioxidant capacity of saliva. In this study, the total antioxidant capacity of saliva has been compared in normal smokers, heavy smokers and non-smokers. Material and methods: In this cross-sectional study, 28 heavy male smokers (more than one pack of cigarettes per day), 28 normal male smokers (less than one pack of cigarettes per day), and 28 male non-smokers aged 25 to 40 years old entered the study. Unstimulated saliva was collected by Spitting method. The total antioxidant capacity of saliva was measured using Ferric Reducing Antioxidant Power Assay. Descriptive statistics, ANOVA, and Tukey tests were used to analyze the data. Results: The mean total antioxidant capacity of saliva in male non-smokers was 0.0598 ± 0.08 ?mol / L, in normal male smokers was 0.049 ± 0.04 ?mol / L, and in heavy male smokers was 0.0388 ± 0.035 ?mol / L, which did not show any significant difference between the groups (P > 0.05). Conclusion: The results of this study indicate that smoking does not have a significant effect on total antioxidant capacity of saliva in smokers. KEYWORDS Smokers; Saliva; Total antioxidant capacity.  

scholarly journals Agronomic characteristics and phytochemical profiles of advanced June-bearing strawberry lines for the northern Canadian climate

2014 ◽  
Vol 23 (1) ◽  
pp. 38-47 ◽  
Author(s):  
Zhichun Xie ◽  
Jinshuan Fan ◽  
Denis Charlebois ◽  
Dominique Roussel ◽  
Claudine Dubé ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Oxygen Radical ◽  
Oxygen Radical Absorbance Capacity ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Total Antioxidant ◽  
Ferric Reducing Antioxidant Power

Eleven advanced strawberry lines (‘SJ011‑10’, ‘SJ0440‑2’, ‘SJ061‑8’, ‘SJ066‑3’, ‘SJ069‑3’, ‘SJ069‑12’, ‘SJ0814‑37’, ‘SJ8518‑11’, ‘K04‑12’, ‘LL0220‑10’ and ‘V151’) were evaluated for their yield, fruit quality, total phenolic content, total antioxidant capacity, and phenolic composition, and were compared with a commercial cultivar (‘Wendy’). The results showed that ‘SJ069‑3’ had excellent soluble solids content, mid-level titratable acidity, low weight loss, and the best firmness among all the cultivars. Higher total antioxidant capacity was found in ‘SJ069‑3’, according to ferric reducing antioxidant power and oxygen radical absorbance capacity assays, an indication that ‘SJ069‑3’ is a promising new cultivar for the fresh market. In addition to rich individual phenolics, ‘SJ061‑8’ had the highest total antioxidant capacity, which was significantly different from the other genotypes, suggesting the potential use of this line as parent material in breeding or as a functional food ingredient. There was a strong relationship between total antioxidant capacity and total phenolic content, according to Folin–Ciocalteu, ferric reducing antioxidant power, and oxygen radical absorbance capacity assays. This study confirms that anthocyanins are major phenolic compounds contributing to the main antioxidant power of strawberries.

scholarly journals Estimation of Antioxidant Potential of Indigenous Halari and French Poitu Donkey Milk by using the Total Antioxidant Capacity and Ferric Reducing Antioxidant Power Essay

2019 ◽  
Vol 38 (04) ◽  
Author(s):  
Anuradha Bhardwaj ◽  
Parbha Kumari ◽  
Varij Nayan ◽  
Tanvi Sonali ◽  
R A Legha ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Antioxidant Potential ◽  
Donkey Milk ◽  
Frap Assay ◽  
Antioxidant Power ◽  
Reducing Ability ◽  
Aging Properties ◽  
Total Antioxidant ◽  
Ferric Reducing Antioxidant Power

Over the last decade, research has been conducted towards the uses and properties of donkey milk that has distinct chemical composition and consequently particularly nutritional properties. It is interesting to determine antioxidant potential of indigenous Halari and French Poitu donkey milk by using the total antioxidant capacity and ferric reducing antioxidant power assay. These methods are reliable, fast and with sound methodological infrastructures. The objective of the present research was to investigate the applicability of the trolox based total antioxidant capacity (TAC) and ferric reducing ability of plasma (FRAP) assay for identification of antioxidant potential of Indian Halari donkey milk and French Poitu donkey milk. The donkey milk is quite popular for its anti-aging properties in Western and European countries as cosmetic and nutraceutical preparations. However, in Indian context no such study is conducted on Halari donkey milk. The Indian Halari donkeys are untapped resource for their dairy potential. Our results showed that the whole milk of Halari donkey is at par with French Poitu donkey milk in terms of their antioxidative potential.

Determination of total antioxidant capacity of milk by CUPRAC and ABTS methods with separate characterisation of milk protein fractions

2015 ◽  
Vol 82 (2) ◽  
pp. 177-184 ◽  
Author(s):  
Sema Demirci Çekiç ◽  
Aslı Demir ◽  
Kevser Sözgen Başkan ◽  
Esma Tütem ◽  
Reşat Apak
Keyword(s):  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Milk Protein ◽  
Skim Milk ◽  
Protein Fractions ◽  
Relative Standard Error ◽  
Antioxidant Power ◽  
Antioxidant Capacities ◽  
Relative Standard ◽  
Total Antioxidant

Most milk-applied antioxidant assays in literature are based on the isolation and quantification of individual antioxidative compounds, whereas total antioxidant capacity (TAC) gives a more holistic picture due to cooperative action of antioxidants. Recently, the cupric reducing antioxidant capacity (CUPRAC) method has been modified to measure the antioxidant capacities of thiol-containing proteins, where the classical ammonium acetate buffer – that may otherwise precipitate proteins– was replaced with concentrated urea buffer (able to expose embedded thiol groups of proteins to oxidative attack) adjusted to pH 7.0. Thus, antioxidant capacity of milk was investigated with two competing TAC assays, namely CUPRAC and ABTS (2,2′-azinobis(3-ethylbenzothiazoline-6-sulphonic acid))/persulphate, because only these assays were capable of evaluating protein contribution to the observed TAC value. As milk fat caused turbidity, experiments were carried out with skim milk or defatted milk samples. To determine TAC, modified CUPRAC method was applied to whole milk, separated and redissolved protein fractions, and the remaining liquid phase after necessary operations. Both TAC methods were investigated for their dilution sensitivity and antioxidant power assessment of separate milk fractions such as casein and whey. Proteins like β-lactoglobulin and casein (but not simple thiols) exhibited enhanced CUPRAC reactivity with surfactant (SDS) addition. Addition of milk protein fractions to whole skim milk produced significant ‘negative-biased’ deviations (up to −26% relative standard error) from TAC absorbance additivity in the application of the ABTS method, as opposed to that of the CUPRAC method less affected by chemical deviations from Beer's law thereby producing much smaller deviations from additivity (i.e. the property of additivity is valid when the measured TAC of a mixture is equal to the sum of individual antioxidant capacities of its constituents).

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