Synthesis of13C-Labeled Patulin [4-Hydroxy-4H-furo[3,2-c]pyran-2(6H)-one] To Be Used as Internal Standard in a Stable Isotope Dilution Assay

1998 ◽  
Vol 46 (12) ◽  
pp. 5163-5169 ◽  
Author(s):  
M. Rychlik ◽  
P. Schieberle
Keyword(s):  
Stable Isotope ◽  
Isotope Dilution ◽  
Internal Standard ◽  
Stable Isotope Dilution Assay ◽  
Stable Isotope Dilution ◽  
Isotope Dilution Assay ◽  
Dilution Assay

scholarly journals Quantitative Analysis of 2-Aminoacetophenone in Off-Flavored Wines by Stable Isotope Dilution Assay

1996 ◽  
Vol 79 (2) ◽  
pp. 583-586 ◽  
Author(s):  
Bettina Dollmann ◽  
Dominicus Wichmann ◽  
Alfred Schmitt ◽  
Hans Koehler ◽  
Peter Schreier
Keyword(s):  
Quantitative Analysis ◽  
Stable Isotope ◽  
Isotope Dilution ◽  
Internal Standard ◽  
Stable Isotope Dilution Assay ◽  
Isotope Dilution Analysis ◽  
Model Experiments ◽  
Stable Isotope Dilution ◽  
Isotope Dilution Assay ◽  
Dilution Assay

Abstract Isotope dilution analysis was used to quantitate 2- aminoacetophenone in wines exhibiting the so- called untypical aging off-flavor. d3-Aminoacetophe- none was synthesized and used as isotopomeric internal standard. The method of quantitation was verified by several model experiments. In the off-flavored wines studied, amounts of 2-aminoacetophe none ranging from 0.7 to 12.8 μg/L were determined.

Quantitation of Mycotoxins in Four Food Matrices Comparing Stable Isotope Dilution Assay (SIDA) with Matrix-Matched Calibration Methods by LC–MS/MS

2019 ◽  
Vol 102 (6) ◽  
pp. 1673-1680 ◽  
Author(s):  
Dan Li ◽  
Justin A Steimling ◽  
Joseph D Konschnik ◽  
Scott L Grossman ◽  
Ty W Kahler
Keyword(s):  
Stable Isotope ◽  
Reference Materials ◽  
Isotope Dilution ◽  
Matrix Effects ◽  
Internal Standard ◽  
Stable Isotope Dilution Assay ◽  
Stable Isotope Dilution ◽  
Isotope Dilution Assay ◽  
Dilution Assay ◽  
Calibration Methods

Abstract Background: Mycotoxins are big concerns in food safety. Analytical methods are important for the evaluation of mycotoxins in different food commodities. Objective: In this study, stable isotope dilution assay (SIDA) was compared with a matrix-matched calibration method for the quantification of mycotoxins in four different commercially available commodities and two reference materials. Methods: All samples were extracted with water–acetonitrile (50+50, v/v), followed by filtration and LC–tandem MS analysis. Results: SIDA calibration accuracies ranged from 78.6 to 112% with relative SDs (RSDs) ≤16% across all four matrices. The majority of the recoveries across all matrices ranged from 70 to 120% with RSDs <11%. Of the four mycotoxins in the reference materials analyzed, only three had 13C-internal standard (IS), whereas the fourth was quantified using a closely eluting 13C-IS for a different mycotoxin. Mycotoxins paired with their corresponding 13C-IS had accuracies >90%, whereas accuracies for the mismatched mycotoxin/13C-IS were <14%. Conclusions: When 13C-IS are not available, matrix-matched calibration was also evaluated as an alternative to quantitating target mycotoxins. The use of 13C-IS is the best way to dynamically account for prevalent matrix effects, but matrix matching provides a viable alternative. Highlights: The study compared SIDA and matrix-matched calibration methods in terms of recovery, efficiency, advantages, and limitations for LC-MS based mycotoxin analysis.

Quantitation of Mycotoxins in Four Food Matrices Comparing Stable Isotope Dilution Assay (SIDA) with Matrix-Matched Calibration Methods by LC–MS/MS

2019 ◽  
Vol 102 (6) ◽  
pp. 1673-1680
Author(s):  
Dan Li ◽  
Justin A. Steimling ◽  
Joseph D. Konschnik ◽  
Scott L. Grossman ◽  
Ty W. Kahler
Keyword(s):  
Stable Isotope ◽  
Reference Materials ◽  
Isotope Dilution ◽  
Matrix Effects ◽  
Internal Standard ◽  
Stable Isotope Dilution Assay ◽  
Stable Isotope Dilution ◽  
Isotope Dilution Assay ◽  
Dilution Assay ◽  
Calibration Methods

Background: Mycotoxins are big concerns in food safety. Analytical methods are important for the evaluation of mycotoxins in different food commodities. Objective: In this study, stable isotope dilution assay (SIDA) was compared with a matrix-matched calibration method for the quantification of mycotoxins in four different commercially available commodities and two reference materials. Methods: All samples were extracted with water–acetonitrile (50+50, v/v), followed by filtration and LC–tandem MS analysis. Results: SIDA calibration accuracies ranged from 78.6 to 112% with relative SDs (RSDs) ≤16% across all four matrices. The majority of the recoveries across all matrices ranged from 70 to 120% with RSDs <11%. Of the four mycotoxins in the reference materials analyzed, only three had 13C-internal standard (IS), whereas the fourth was quantified using a closely eluting 13C-IS for a different mycotoxin. Mycotoxins paired with their corresponding 13C-IS had accuracies >90%, whereas accuracies for the mismatched mycotoxin/13C-IS were <14%. Conclusions: When 13C-IS are not available, matrix-matched calibration was also evaluated as an alternative to quantitating target mycotoxins. The use of 13C-IS is the best way to dynamically account for prevalent matrix effects, but matrix matching provides a viable alternative. Highlights: The study compared SIDA and matrix-matched calibration methods in terms of recovery, efficiency, advantages, and limitations for LC-MS based mycotoxin analysis.

Quantitation of Rotundone in Grapefruit (Citrus paradisi) Peel and Juice by Stable Isotope Dilution Assay

2017 ◽  
Vol 65 (24) ◽  
pp. 5026-5033 ◽  
Author(s):  
Akira Nakanishi ◽  
Yusuke Fukushima ◽  
Norio Miyazawa ◽  
Keisuke Yoshikawa ◽  
Tomoko Maeda ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Isotope Dilution ◽  
Stable Isotope Dilution Assay ◽  
Citrus Paradisi ◽  
Stable Isotope Dilution ◽  
Isotope Dilution Assay ◽  
Dilution Assay

Quantitative Analysis of Beer Aromatic Alcohols Using Stable Isotope Dilution Assay

2002 ◽  
Vol 60 (2) ◽  
pp. 88-96 ◽  
Author(s):  
Jean-Pierre Dufour ◽  
Rana Wierda ◽  
Michelle Leus ◽  
Geert Lissens ◽  
Freddy Delvaux ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Stable Isotope ◽  
Isotope Dilution ◽  
Stable Isotope Dilution Assay ◽  
Stable Isotope Dilution ◽  
Isotope Dilution Assay ◽  
Dilution Assay ◽  
Aromatic Alcohols

Development of a Stable Isotope Dilution Assay for Tenuazonic Acid

2011 ◽  
Vol 59 (7) ◽  
pp. 2980-2987 ◽  
Author(s):  
Stefan Asam ◽  
Yang Liu ◽  
Katharina Konitzer ◽  
Michael Rychlik
Keyword(s):  
Stable Isotope ◽  
Isotope Dilution ◽  
Stable Isotope Dilution Assay ◽  
Tenuazonic Acid ◽  
Stable Isotope Dilution ◽  
Isotope Dilution Assay ◽  
Dilution Assay
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