Food Folate Assay with Protease, α-Amylase, and Folate Conjugase Treatments

1997 ◽  
Vol 45 (1) ◽  
pp. 135-139 ◽  
Author(s):  
Tsunenobu Tamura ◽  
Yasuharu Mizuno ◽  
Kelley E. Johnston ◽  
Robert A. Jacob
Keyword(s):  
2008 ◽  
Vol 108 (9) ◽  
pp. A35 ◽  
Author(s):  
M.E. Payne ◽  
B.D. Jamerson ◽  
C.F. Potocky ◽  
A.E. Ashley-Koch ◽  
M.C. Speer ◽  
...  

2014 ◽  
Vol 27 (3) ◽  
pp. 321-327
Author(s):  
Lívia de Castro Crivellenti ◽  
Patrícia Barbieri ◽  
Daniela Saes Sartorelli

OBJECTIVE: To estimate food and dietary folate inadequacies in the diets of adult pregnant women. METHODS: A prospective study was conducted with 103 healthy pregnant adult users of the Public Health Care System of Ribeirão Preto, São Paulo, Brazil. The present study included the 82 women with complete food intake data during pregnancy, which were collected by three 24-hour dietary recalls. Food folate (folate naturally present in foods) and dietary folate (food folate plus folate from fortified wheat flour and cornmeal) inadequacies were determined, using the Estimated Average Requirement as cutoff. RESULTS: The diets of 100% and 94% of the pregnant women were inadequate in food folate and dietary folate, respectively. However, fortified foods increased the medium availability of the nutrient by 87%. CONCLUSION: The large number of pregnant women consuming low-folate diets was alarming. Nationwide population studies are needed to confirm the hypothesized high prevalence of low-folate diets among pregnant women.


2006 ◽  
Vol 17 (11) ◽  
pp. 728-734 ◽  
Author(s):  
Jean Hung ◽  
Tai Li Yang ◽  
Tania F. Urrutia ◽  
Rui Li ◽  
Cydne A. Perry ◽  
...  

2008 ◽  
Vol 101 (2) ◽  
pp. 206-212 ◽  
Author(s):  
Leane Hoey ◽  
Helene McNulty ◽  
Elizabeth M. E. McCann ◽  
Kelvin J. McCracken ◽  
John M. Scott ◽  
...  

There are few good sources of natural food folates apart from green leafy vegetables and these may have a limited potential to increase folate status because of substantial losses that can occur during cooking. Fortified foods can overcome this but are controversial because of safety concerns regarding chronic exposure to high-dose folic acid (FA; the synthetic form). The aim of the present study was to develop eggs with an enriched natural folate content and minimal unmetabolised FA. Forty-eight, 30-week-old laying hens were randomised to receive the basal feed (formulated to provide 1 mg folate/kg feed) to which had been added one of the following FA levels (0, 2, 4, 8, 16, 32 mg/kg feed). Total folate was measured in eggs collected throughout the 12-week study period and the FA content estimated at 12 weeks. Results showed that the maximal egg folate content was achieved by adding 16 mg FA/kg feed. At this optimal dose, the total folate content per egg was 75 μg (compared with 32 μg in a regular egg) of which FA represented at most 10 %, a level which would probably be converted into natural folates by humans after ingestion. The results demonstrate that it is possible to use synthetic FA at high doses to produce novel animal foods enriched with natural folates in a cost-efficient process. Such foods may be particularly relevant to European populations without access to FA fortification and therefore dependent on natural food folate sources for the primary prevention of folate-related disease.


2002 ◽  
Vol 88 (3) ◽  
pp. 235-242 ◽  
Author(s):  
Erik J. M. Konings ◽  
Freddy J. Troost ◽  
Jacqueline J. M. Castenmiller ◽  
Harry H. S. Roomans ◽  
Piet A. van den Brandt ◽  
...  

Our knowledge on the absorption of folate is incomplete. The deconjugation process as a possible limiting factor in the absorption of folates was investigated. The study also attempted to validate the use of the area under the serum response curve (AUC) from food compared with folic acid as a proxy variable for food folate bioavailability. Folate absorption was determined in healthy ileostomy volunteers (n11) using a single-dose short-term protocol. In a randomised crossover design, volunteers received spinach meals and a supplement. Based on analysis of test meals and ileostomy effluents, there was no difference in folate absorption between spinach with a mono-:polyglutamate ratio 40:60 and the same spinach with a 100:0 ratio. The absolute absorption of spinach folate (79 %) calculated from the difference between folate intake and folate content of ileostomy effluents was approximately equal to the relative absorption (81 %) calculated from the AUC after consumption of spinach meals in relation to the AUC after consumption of the folic acid supplement. We conclude that the deconjugation process is not a limiting factor in the absorption of spinach folates. Comparison of AUC of food folatev.folic acid in a short-term protocol may be suitable for assessing food folate bioavailability.


1999 ◽  
Vol 129 (4) ◽  
pp. 913-916 ◽  
Author(s):  
Reinhild Prinz-Langenohl ◽  
Anja Brönstrup ◽  
Barbara Thorand ◽  
Monika Hages ◽  
Klaus Pietrzik
Keyword(s):  

Nutrients ◽  
2015 ◽  
Vol 7 (4) ◽  
pp. 2663-2686 ◽  
Author(s):  
Claire Marchetta ◽  
Owen Devine ◽  
Krista Crider ◽  
Becky Tsang ◽  
Amy Cordero ◽  
...  

1973 ◽  
Vol 25 (4) ◽  
pp. 513-532 ◽  
Author(s):  
T. Tamura ◽  
E. L. R. Stokstad
Keyword(s):  

1999 ◽  
Vol 99 (3) ◽  
pp. 285 ◽  
Author(s):  
CAROL WEST SUITOR ◽  
LYNN B BAILEY
Keyword(s):  

Sign in / Sign up

Export Citation Format

Share Document