Method for Extraction and Separation by Solid Phase Extraction of Neutral Lipid, Free Fatty Acids, and Polar Lipid from Mixed Microbial Cultures†

1997 ◽  
Vol 45 (1) ◽  
pp. 132-134 ◽  
Author(s):  
H. Gale Bateman ◽  
Thomas C. Jenkins
Keyword(s):  
Fatty Acids ◽  
Solid Phase Extraction ◽  
Free Fatty Acids ◽  
Neutral Lipid ◽  
Polar Lipid ◽  
Solid Phase ◽  
Phase Extraction ◽  
Extraction And Separation ◽  
Microbial Cultures ◽  
Mixed Microbial Cultures

scholarly journals Identification and Quantification of Fatty Acids in T. viridissima, C. biguttulus, and C. brunneus by GC-MS

2018 ◽  
Vol 2018 ◽  
pp. 1-8
Author(s):  
Alexander M. Wathne ◽  
Hanne Devle ◽  
Carl Fredrik Naess-Andresen ◽  
Dag Ekeberg
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Solid Phase Extraction ◽  
Free Fatty Acids ◽  
Dry Matter ◽  
Solid Phase ◽  
Neutral Lipids ◽  
Methyl Esters ◽  
Phase Extraction ◽  
Chorthippus Biguttulus

Fatty acid (FA) profiles of the species Tettigonia viridissima, Chorthippus biguttulus, and Chorthippus brunneus were determined and quantitated. Extracted lipids were derivatized into FA methyl esters (FAMEs) prior to analysis by GC-MS. A total of 37 different FAs were identified in T. viridissima, yielding a total FA content of 10.4 g/100 g of dry matter. The contents of saturated FAs, monounsaturated FAs, and polyunsaturated FAs were 31.1, 35.9, and 33.0%, respectively. Lipids from T. viridissima were also fractioned into neutral lipids, free fatty acids, and polar lipids by offline solid phase extraction. For C. brunneus and C. biguttulus, 33 FAs were identified, yielding a total FA content of 6.14 g/100 g of dry matter. SFAs, MUFAs, and PUFAs, respectively, constituted 32.7, 25.1, and 42.1% of the total FA content. The contents of MUFAs, PUFAs, n-3 FAs, and n-6 FAs of each species, and the n-6/n-3 ratio, were subsequently discussed.

Analysis of Free Fatty Acids in Beer: Comparison of Solid-Phase Extraction, Solid-Phase Microextraction, and Stir Bar Sorptive Extraction

2009 ◽  
Vol 57 (23) ◽  
pp. 11081-11085 ◽  
Author(s):  
Tomáš Horák ◽  
Jiří Čulík ◽  
Pavel Čejka ◽  
Marie Jurková ◽  
Vladimír Kellner ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Solid Phase Extraction ◽  
Free Fatty Acids ◽  
Solid Phase Microextraction ◽  
Solid Phase ◽  
Stir Bar Sorptive Extraction ◽  
Phase Extraction ◽  
Sorptive Extraction
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