Enzymatic Hydrolysis of Milk Proteins Used for Emulsion Formation. 1. Kinetics of Protein Breakdown and Storage Stability of the Emulsions

1996 ◽  
Vol 44 (11) ◽  
pp. 3631-3636 ◽  
Author(s):  
Samson O. Agboola ◽  
Douglas G. Dalgleish
Keyword(s):  
Enzymatic Hydrolysis ◽  
Storage Stability ◽  
Milk Proteins ◽  
Protein Breakdown ◽  
And Storage ◽  
Kinetics Of ◽  
Hydrolysis Of

scholarly journals Kinetics of Enzymatic Hydrolysis of Southeast Sulawesi Sago Starch

2020 ◽  
pp. 53-61
Author(s):  
Ansharullah Ansharullah ◽  
Muhammad Natsir
Keyword(s):  
Enzymatic Hydrolysis ◽  
Maximum Velocity ◽  
Enzyme Concentration ◽  
Hydrolysis Rate ◽  
Sago Starch ◽  
Optimum Conditions ◽  
Kinetics Of ◽  
Hydrolysis Of ◽  
Substrate Concentrations ◽  
Menten Constant

The aims of this study were to characterize the kinetics of enzymatic hydrolysis of sago starch, obtained from Southeast Sulawesi Indonesia. The enzyme used for hydrolysis was bacterial ∝-amylase (Termamyl 120L from Bacillus licheniformis, E. C. 3.2.1.1).  The method to determine the initial velocity (Vo) of the hydrolysis was developed by differentiation a nonlinear equation (NLE).  The Vo of the hydrolysis was measured at various pH (6.0, 6.5,and 7.0), temperatures (40, 60, 75 and 95oC), enzyme concentrations (0.5, 1.0, 1.5 and 2.0 µg per mL) and in the presence of 70 ppm Ca++. The optimum conditions of this experiment were found to be at pH 6.5 – 7.0 and 75oC, and the Vo increased with increasing enzyme concentration. The Vo values at various substrate concentrations were also determined, which were then used to calculate the enzymes kinetics constant of the hydrolysis, including Michaelis-Menten constant (Km) and maximum velocity (Vmax) using a Hanes plot.  Km and Vmax values were found to be higher in the measurement at pH 7.0 and 75oC. The Km values  at four  different combinations of pH and temperatures (pH 6.5, 40oC; pH 6.5, 75oC; pH 7.0, 40oC; pH 7.0, 75oC) were found to be 0.86, 3.23, 0.77 and 3.83 mg/mL, respectively; and Vmax values were 17.5, 54.3, 20.3 and 57.1 µg/mL/min, respectively. The results obtained showed that hydrolysis rate of this starch was somewhat low.

scholarly journals Kinetics of enzymatic hydrolysis of polysaccharide-rich particulates

2007 ◽  
Vol 38 (1) ◽  
pp. 21-27 ◽  
Author(s):  
Pin-Jing He ◽  
Fan Lü ◽  
Li-Ming Shao ◽  
Xiu-Jiang Pan ◽  
Duu-Jong Lee
Keyword(s):  
Enzymatic Hydrolysis ◽  
Kinetics Of ◽  
Hydrolysis Of

Kinetics of enzymatic hydrolysis of olive oil in biphasic organic-aqueous systems

1991 ◽  
Vol 38 (7) ◽  
pp. 761-766 ◽  
Author(s):  
Shau-Wei Tsai ◽  
Guang-Huei Wu ◽  
Chen-Li Chiang
Keyword(s):  
Enzymatic Hydrolysis ◽  
Olive Oil ◽  
Aqueous Systems ◽  
Kinetics Of ◽  
Hydrolysis Of
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