Corn Steep Liquor as a Nutrition Adjunct for the Production of Aspergillus niger Lipase and Hydrolysis of Oils Thereof

2009 ◽  
Vol 57 (22) ◽  
pp. 10658-10663 ◽  
Author(s):  
N. G. Edwinoliver ◽  
K. Thirunavukarasu ◽  
S. Purushothaman ◽  
C. Rose ◽  
M. K. Gowthaman ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Corn Steep Liquor ◽  
Steep Liquor ◽  
Hydrolysis Of

scholarly journals Optimum conditions for Inulinase production by Aspergillus niger using solid state fermentation

2015 ◽  
Vol 12 (2) ◽  
pp. 307-316 ◽  
Author(s):  
Baghdad Science Journal
Keyword(s):  
Aspergillus Niger ◽  
Specific Activity ◽  
Corn Steep Liquor ◽  
Optimum Conditions ◽  
Inulin Hydrolysis ◽  
Enzyme Productivity ◽  
Initial Ph ◽  
Quantitative Screening ◽  
Steep Liquor ◽  
Fungal Colony

Thirty local fungal isolates according to Aspergillus niger were screened for Inulinase production on synthetic solid medium depending on inulin hydrolysis appear as clear zone around fungal colony. Semi-quantitative screening was performed to select the most efficient isolate for inulinase production. the most efficient isolate was AN20. The optimum condition for enzyme production from A. niger isolate was determined by busing a medium composed of sugar cane moisten with corn steep liquor 5;5 (v/w) at initial pH 5.0 for 96 hours at 30 0C . Enzyme productivity was tested for each of the yeast Kluyveromyces marxianus, the fungus A. niger AN20 and for a mixed culture of A. niger and K. marxianus. The productivity of A. niger gave the highest specific activity of 153 U/mg, as compared with K. marxianus which gave 86 U/mg.

Hydrolysis of Corncob Residue by Enzymes from Fungi C1067 and the Products Analysis by Capillary Electrophoresis

2011 ◽  
Vol 145 ◽  
pp. 68-72
Author(s):  
Jian Lu Ma ◽  
Xiao Min Fang ◽  
Xin Jiong Guo ◽  
Huan Fei Liu ◽  
Gui Fu Dai ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Corn Steep Liquor ◽  
Nitrogen Sources ◽  
Fermentation Medium ◽  
Citrate Buffer ◽  
Carbon And Nitrogen ◽  
Products Analysis ◽  
Steep Liquor ◽  
Hydrolysis Of ◽  
Assay Conditions

To achieve high enzymatic hydrolytic efficiency of corncob residue, one strain, Trichoderma C1067, with the enzyme activity of corncob residue as high as 4.5g/L glucose liberated per hour in 0.5mL crude cellulase and 0.5mL citric acid /sodium citrate buffer under the assay conditions, was carefully studied. The optimal carbon and nitrogen sources used in the fermentation medium are as follows: corncob residues 2.0%, wheat bran 1.5%, corncob 0.5%, rice straw 0.5%, peptone 0.1% and corn steep liquor 4%. The optimal conditions of saccharification for corncob are 55°C and pH 4.0. The sugars gained in the hydrolysate consist of glucose 4.1% and xylose 0.4% after enzymolysis for 72h, tested by capillary electrophoresis.

MICROBIAL PENTOSANASES: III. SOME FACTORS AFFECTING THE PRODUCTION OF PENTOSANASES BY ASPERGILLUS NIGER AND TRICHODERMA VIRIDE

1959 ◽  
Vol 5 (1) ◽  
pp. 99-107 ◽  
Author(s):  
F. J. Simpson
Keyword(s):  
Aspergillus Niger ◽  
Corn Steep Liquor ◽  
Synthetic Medium ◽  
Trichoderma Viride ◽  
Maximum Yield ◽  
Ammonium Hydroxide ◽  
Water Soluble ◽  
Factors Affecting ◽  
Steep Liquor ◽  
Mesh Sieve

Of a number of carbohydrates tested, holocellulose from wheat straw and L-arabinose were the better substrates for production of pentosanase by Trichoderma viride. D-Xylose did not induce synthesis of the enzyme by the fungus. Production in a synthetic medium was increased by the addition of malt sprouts, distillers' dried solubles, or glutamic acid.Under the same conditions the production of pentosanase by Aspergillus niger was favored by the water-soluble pentosan of wheat flour, holocellulose, and wheat bran. Both D-xylose and L-arabinose induced synthesis of enzyme. In the synthetic medium the addition of yeast extract, corn steep liquor, malt sprouts, or a number of other nitrogenous adjuncts increased the yield of pentosanase. A medium containing 3% bran ground to pass a 20-mesh sieve, 3% corn steep liquor neutralized with ammonium hydroxide, and 1% calcium carbonate was developed for the production of pentosanase by A. niger. Maximum yield was obtained in 60 hours. The pentosanase had an optimum pH of 5.0 and was stable for 30 minutes at 30 °C between pH 4.0 and 5.8. The pentosanase could be precipitated from the culture filtrates with 76% ethanol and when stored as a dry powder at 2 °C was stable for at least 1 year.

scholarly journals Statistical optimization of an acid protease production by a local Aspergillus niger MH109542 using a medium based on decommissioned dates

2020 ◽  
pp. 68-76
Author(s):  
Malika Benkahoul ◽  
Amina Bramki ◽  
Aicha Belmessikh ◽  
Aicha Mechakra-Maza
Keyword(s):  
Aspergillus Niger ◽  
Yeast Extract ◽  
Corn Steep Liquor ◽  
Basal Medium ◽  
Extreme Environment ◽  
Fermentation Medium ◽  
Acid Protease ◽  
Protease Production ◽  
Plackett And Burman ◽  
Steep Liquor

The production of an acid protease by liquid fermentation is performed on a medium based on decommissioned dates by a local mold isolated from an extreme environment. The used mold is isolated from the thermal soil in Hammam Safsaf of Teleghma (Algeria). Phenotypic and molecular identification has shown that it is Aspergillus niger. This fungi strain exhibited exo-protease activity on milk agar. The fermentation medium based on decommissioned dates is enriched by other factors according to a statistical method; the plan of Plackett and Burman. This method (N = 8 experiments and N-1 factors) allowed the optimization of the enzyme production and the growth of the mold. The statistical analysis of the obtained results shows an increased enzyme activity (650.20U), in the presence of yeast extract and salts with probabilities p = 0.239 for the first and p = 0.190 for the second. Furthermore, the corn-steep-liquor (p = 0.229) and yeast extract (p = 0.053) present a significant effect on mold growth. In conclusion, the culture of A. niger on optimized medium gives good yields of biomass and proteolytic activity compared to the basal medium. These results are encouraging. In fact, the use of a cheap and available substrate such as decommissioned dates saves the cost price of fermentation on an industrial scale.

Utilization of Orange Peel, Corn Steep Liquor, and Parboiled Rice Water in the Production of Polygalacturonase from Aspergillus niger

2015 ◽  
Vol 11 (5) ◽  
pp. 284-291 ◽  
Author(s):  
Jonaina Pili ◽  
Angélica Danielli ◽  
Jamile Zeni ◽  
Márcia Maria Santin Trentini ◽  
Rogério Luis Cansian ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Corn Steep Liquor ◽  
Orange Peel ◽  
Parboiled Rice ◽  
Rice Water ◽  
Steep Liquor

scholarly journals APROVEITAMENTO DA ÁGUA DE MACERAÇÃO DE MILHO PARA PRODUÇÃO DE COMPOSTOS BIOATIVOS POR ASPERGILLUS NIGER (UCP/WFCC 1261)

e-xacta ◽  
2015 ◽  
Vol 8 (1) ◽  
Author(s):  
Raphael de Araújo Luz Nascimento ◽  
Maria Helena MenezesEstevam Alves ◽  
José Henrique EdmilsonSouza Freitas ◽  
Layla Carvalho Manhke ◽  
Marcos Antônio Cavalcanti Luna ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Corn Steep Liquor ◽  
Steep Liquor

<p align = "justify">O objetivo da presente proposta foi avaliar a produção de glicosamina, ácido citrico, enzimas ligninolíticas, protease e polifosfato por um isolado de Aspergillus niger obtido do solo da caatinga, em resposta à presença da água de maceração do milho, “corn steep liquor”, um subproduto da cadeia produtiva do milho, que atua como fonte de nitrogênio para crescimento microbiano. Os efeitos da presença do metanol no meio de cultura foram também avaliados. O fungo foi cultivado em meio glicose-peptona, como controle, e em meio contendo água de maceração em substituição à peptona, a 150 rpm, a 30 ºC durante 168 horas. Amostras foram coletadas a intervalos de 24 horas. O sobrenadante da cultura foi separado por centrifugação a 10.000 x g a 4 ºC. A biomassa foi utilizada para determinação da biomassa por peso seco, glucosamina, polifosfato e estudo morfológico sob a microscopia de luz. O sobrenadante foi utilizado para a determinação de ácido cítrico, celulase, celobiase (β-glicosidase), exoglucanase, xilanase e protease. Os ensaios foram realizados em cinco réplicas, e os resultados apresentados como média dos dados obtidos. Os resultados foram avaliados empregando-se a análise de variância e as diferenças significativas entre as médias (p ≤ 0,05) determinadas pelo teste de Tukey, com auxílio do software STATISTIC 7.0 . Os resultados obtidos revelaram os efeitos significativos do subproduto sobre a produção dos bioativos avaliados. Um conteúdo de glicosamina de 4,68 g g-1 de biomassa seca foi obtido para o crescimento em CSL mais metanol, comparado a 2,36 g g-1 para as culturas controle crescidas em glicose e peptona mais metanol. Um rendimento de ácido citrico de 2,32 g L-1 foi determinado para células cultivadas na presença de CSL/metanol, comparado a 1,54 g L-1 para células cultivadas em meio contendo glicose, peptona e metanol. A presença do metanol 1,5 % no meio de cultura aumentou o conteúdo dos compostos de forma significativa. Atividades correspondentes a 0,996 U mL-1, 0,488 U mL-1 e 0,52 U mL-1 foram determinadas para celulase, celobiase e exoglucanase, respectivamente, para as células cultivadas em meio CSL, comparado a 0,758 U mL-1, 0,342 U mL-1 e 0,40 U mL-1 para as células crescidas em glicose peptona. Para a xilanase, atividades de 45,27 U mL-1 e 39,99 U mL-1 foram determinadas para CSL e glicose peptona, respectivamente. Os dados revelaram que o crescimento celular em metanol resultou na redução da atividade das enzimas ligninolíticas. Atividades de 3,86 U mL-1 e 2,53 U mL-1 foram obtidas para as proteases nos cultivos em CSL e glicose e peptona mais metanol, respectivamente. Foi observado um aumento no conteúdo do polifosfato durante 96 horas de cultivo. Após esse período, o conteúdo do polímero diminuiu, indicando sua degradação nas condições testadas. Esse estudo também revelou que as células cultivadas na presença de CSL e metanol apresentaram alterações morfológicas. O isolado mostra-se útil para estudos contínuos na busca pela otimização da produção dos compostos bioativos avaliados. Os resultados podem ser valiosos para subsidiar o entendimento do comportamento celular frente a modificações e consequentemente no desenvolvimento de processos de produção mais eficientes e na redução de custos.</p>

scholarly journals Effects of Organic Fertilizer on Bok Choy Growth and Quality in Hydroponic Cultures

Agronomy ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 491
Author(s):  
Kazuki Kano ◽  
Hiroaki Kitazawa ◽  
Keitaro Suzuki ◽  
Ani Widiastuti ◽  
Hiromitsu Odani ◽  
...  
Keyword(s):  
Plant Root ◽  
Oxygen Deficiency ◽  
Organic Fertilizer ◽  
Corn Steep Liquor ◽  
Ascorbic Acid Content ◽  
Growth And Yield ◽  
Significant Difference ◽  
Carbon Nitrogen Ratio ◽  
Nitrogen Ratio ◽  
Steep Liquor

Effects of corn steep liquor (organic fertilizer, OF) and conventional chemical fertilizer (CF) on the growth and yield of bok choy (Brassica rapa var. chinensis) in summer and autumn hydroponic growing systems were compared. When OF and CF were applied with the same amount of total nitrogen in summer cultivation, there was no significant difference between yields; however, the growth rate in OF was slower than in CF. When OF was applied with twice the amount of nitrogen in CF (OF2), bok choy growth and yield were significantly inhibited in summer cultivation, likely owing to dissolved oxygen deficiency and different rates of nitrification and nitrogen absorbance by the plant root. Although the contents of potassium, calcium, and magnesium in bok choy showed no difference among the three treatments in both cultivation seasons, the carbon/nitrogen ratio tended to be higher in OF and OF2 than in CF. Lower nitric acid and higher ascorbic acid content was found in OF and OF2 than in CF. Overall, our results suggest that a comparable yield is expected by using the same nitrogen amount with a conventional recipe of chemical fertilization in autumn cultivation. However, further improvement of hydroponic management is needed in summer cultivation.

scholarly journals Improvement of Enzymatic Saccharification of Cellulose-Containing Raw Materials Using Aspergillus niger

Processes ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 1360
Author(s):  
Ekaterina Budenkova ◽  
Stanislav Sukhikh ◽  
Svetlana Ivanova ◽  
Olga Babich ◽  
Vyacheslav Dolganyuk ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Aspergillus Niger ◽  
Filter Paper ◽  
Raw Materials ◽  
Enzymatic Saccharification ◽  
Wood Chip ◽  
Cellulase Activity ◽  
Wood Chips ◽  
Acid Pretreatment ◽  
Hydrolysis Of

Enzymatic hydrolysis of cellulose-containing raw materials, using Aspergillus niger, were studied. Filter paper, secondary cellulose-containing or starch-containing raw materials, miscanthus cellulose after alkaline or acid pretreatment, and wood chip cellulose, were used as substrates. The study focused on a wild A. niger strain, treated, or not (control), by ultraviolet (UV) irradiations for 45, 60, or 120 min (UV45, UV60, or UV120), or by UV irradiation for 120 min followed by a chemical treatment with NaN3 + ItBr for 30 min or 80 min (UV120 + CH30 or UV120 + CH80). A mixture of all the A. niger strains (MIX) was also tested. A citrate buffer, at 50 mM, wasthe most suitable for enzymatic hydrolysis. As the UV exposure time increased to 2 h, the cellulase activity of the surviving culturewas increased (r = 0.706; p < 0.05). The enzymatic activities of the obtained strains, towards miscanthus cellulose, wood chips, and filter paper, were inferior to those obtained with commercial enzymes (8.6 versus 9.1 IU), in some cases. Under stationary hydrolysis at 37 °C, pH = 4.7, the enzymatic activity of A. niger UV120 + CH30 was 24.9 IU. The enzymatic hydrolysis of secondary raw materials, using treated A. niger strains, was themost effective at 37 °C. Similarly, the most effective treatment of miscanthus cellulose and wood chips occurred at 50 °C. The maximum conversion of cellulose to glucose was observed using miscanthus cellulose (with alkaline pretreatment), and the minimum conversion was observed when using wood chips. The greatest value of cellulase activity was evidenced in the starch-containing raw materials, indicating that A. niger can ferment not only through cellulase activity, but also via an amylolytic one.

scholarly journals Effects of corn steep liquor on β-poly(l-malic acid) production in Aureobasidium melanogenum

AMB Express ◽  
2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Genan Wang ◽  
Bingyi Shi ◽  
Pan Zhang ◽  
Tingbin Zhao ◽  
Haisong Yin ◽  
...  
Keyword(s):  
Malic Acid ◽  
Low Cost ◽  
Corn Steep Liquor ◽  
Enrichment Analysis ◽  
Tca Cycle ◽  
Water Soluble ◽  
Growth And Yield ◽  
The Tca Cycle ◽  
Steep Liquor ◽  
Aureobasidium Melanogenum

Abstractβ-poly(l-malic acid) (PMLA) is a water-soluble biopolymer used in medicine, food, and other industries. However, the low level of PMLA biosynthesis in microorganisms limits its further application in the biotechnological industry. In this study, corn steep liquor (CSL), which processes high nutritional value and low-cost characteristics, was selected as a growth factor to increase the PMLA production in strain, Aureobasidium melanogenum, and its metabolomics change under the CSL addition was investigated. The results indicated that, with 3 g/L CSL, PMLA production, cell growth, and yield (Yp/x) were increased by 32.76%, 41.82%, and 47.43%, respectively. The intracellular metabolites of A. melanogenum, such as amino acids, organic acids, and key intermediates in the TCA cycle, increased after the addition of CSL, and the enrichment analysis showed that tyrosine may play a major role in the PMLA biosynthesis. The results presented in this study demonstrated that the addition of CSL would be an efficient approach to improve PMLA production.

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