Comparative in vitro metabolism of tetrachlorvinphos by the soluble fraction (105000g) from sheep, pig, and cow liver homogenates

1979 ◽  
Vol 27 (1) ◽  
pp. 113-116 ◽  
Author(s):  
M. Humayoun Akhtar ◽  
Thomas S. Foster
Keyword(s):  
Soluble Fraction ◽  
In Vitro Metabolism ◽  
Liver Homogenates

The in vitro metabolism of Eulan WA New by liver homogenates from freshwater fish

1986 ◽  
Vol 84 (1) ◽  
pp. 113-117 ◽  
Author(s):  
Andrew Machon ◽  
Michael J. North ◽  
Nicholas C. Price ◽  
David E. Wells
Keyword(s):  
Freshwater Fish ◽  
In Vitro Metabolism ◽  
Liver Homogenates

The effects of cofactor and species differences on the in vitro metabolism of propiophenone and phenylacetone

1981 ◽  
Vol 59 (2) ◽  
pp. 195-201 ◽  
Author(s):  
R. T. Coutts ◽  
D. R. Prelusky ◽  
G. R. Jones
Keyword(s):  
Rat Liver ◽  
In Vitro Metabolism ◽  
Metabolic Route ◽  
Liver Preparation ◽  
Carbonyl Reduction ◽  
Alcohol Dehydrogenation ◽  
Liver Homogenates ◽  
A Minor ◽  
Minor Extent

In vitro metabolism of the aromatic ketone propiophenone and its nonaromatic isomer phenylacetone was studied using fortified 12 000 × g supernatants of liver homogenates from rat and rabbit. Reduction to the corresponding alcohols was the major metabolic route observed, although aliphatic C-hydroxylation and alcohol dehydrogenation also occurred. Marked differences were observed in the amounts of carbonyl reduction of the substrates, which was dependant on the species as well as the cofactor employed. Using rat liver preparation, phenylacetone was reduced to 1-phenyl-2-propanol much more efficiently with an NADH-fortified system than when NADPH was used whereas in rabbit, extensive reduction occurred in the presence of either cofactor. Reduction of propiophenone to 1-phenyl-1-propanol by rat liver preparation was slightly greater in the presence of NADH than with NADPH; the converse was observed in rabbit.Aliphatic hydroxylation of propiophenone to 2-hydroxy-1-phenyl-1-propamine was also a significant metabolic pathway in both species, with NADPH being the more efficient cofactor, but C-1 hydroxylation of phenylacetone to 1-hydroxy-1-phenyl-2-propanone occurred only to a minor extent. Small amounts of 1-phenyl-1,2-propanedione, as well as both erythro and threo isomers of 1-phenyl-1,2-propanediol, were also identified as metabolites in both species. Similar metabolic studies were carried out on the alcohols 1-phenyl-1-propanol and 1-phenyl-2-propanol and again the nature and quantities of metabolites isolated showed both species and cofactor dependancies.

The in vitro metabolism of 2,4-dinitrophenol by rat liver homogenates

1972 ◽  
Vol 21 (2) ◽  
pp. 275-285 ◽  
Author(s):  
Julie L. Eiseman ◽  
Perry J. Gehring ◽  
James E. Gibson
Keyword(s):  
Rat Liver ◽  
In Vitro Metabolism ◽  
Liver Homogenates
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