Quantitative amino acid analysis by gas-liquid chromatography

1971 ◽  
Vol 19 (4) ◽  
pp. 605-618 ◽  
Author(s):  
Charles W. Gehrke ◽  
Robert W. Zumwalt ◽  
Kenneth. Kuo
Keyword(s):  
Liquid Chromatography ◽  
Amino Acid ◽  
Amino Acid Analysis ◽  
Gas Liquid Chromatography ◽  
Quantitative Amino Acid Analysis

7-Desmethyl-Microcystin-RR, a Hepatotoxin from a Waterbloom of Microcystis aeruginosa

1992 ◽  
Vol 47 (5-6) ◽  
pp. 335-340 ◽  
Author(s):  
Cornel Martin ◽  
Jürgen Weckesser ◽  
Tomio Ino ◽  
Wilfried A. König ◽  
Olav M. Skulberg
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Amino Acid ◽  
Microcystis Aeruginosa ◽  
Amino Acid Analysis ◽  
High Performance ◽  
Solid Phase ◽  
Reversed Phase ◽  
Relative Molecular Mass ◽  
Gas Liquid Chromatography

A peptide toxin was isolated from a waterbloom of Microcystis aeruginosa from Lake Froylandsvatn in Norway. The isolation procedure included liquid and solid phase extraction and reversed phase high performance liquid chromatography. Amino acid analysis yielded ᴅ-glutamic acid, ᴅ-erythro-β-methylaspartic acid and ᴅ-alanine in equimolar and ʟ-arginine in twofold molar ratios. The presence of dehydroalanine was confirmed by hydrogenation and subsequent amino acid analysis with combined gas liquid chromatography/mass spectrometry. Investigation of the toxin with fast atom bombardment mass spectrometry showed a nominal relative molecular mass of 1023. 3-Amino-9-m ethoxy-2,6,8-trimethyl-10-phenyl-4,6-decadienoic acid (Adda) was identified by 1H NMR and 1H, 1H COSY spectroscopy. The structure of the toxin was elucidated as 7-desmethyl-microcystin-RR.

Sign in / Sign up

Export Citation Format

Share Document