Extraction procedure for quantitative determination of six elements in plant tissue

1967 ◽  
Vol 15 (2) ◽  
pp. 340-344 ◽  
Author(s):  
John Howard. Baker ◽  
Thomas. Greweling
Keyword(s):  
Quantitative Determination ◽  
Plant Tissue ◽  
Extraction Procedure ◽  
Six Elements

Determination of Rubber in Rubber-Bearing Plants

1934 ◽  
Vol 7 (1) ◽  
pp. 111-124 ◽  
Author(s):  
D. Spence ◽  
M. L. Caldwell
Keyword(s):  
Quantitative Determination ◽  
Systematic Study ◽  
Plant Tissue ◽  
Extensive Investigation ◽  
Present Communication ◽  
Rubber Content ◽  
Essential Step ◽  
Rubber Bearing ◽  
The Subject

Abstract THE determination of the true rubber content of both raw and manufactured rubber has been the subject of extensive investigation. Search has failed to reveal any corresponding record covering studies applicable to rubber-bearing plants. The authors' investigations, covering such methods as have been published, demonstrate their inaccuracy and inadequacy for practical use. There has never been published, as far as the authors have been able to determine, any thorough, systematic study of the factors which determine the value and accuracy of any method for the quantitative determination of the pure caoutchouc contained in a given specimen of plant tissue. The work embodied in the present communication was undertaken as an essential step towards the solution of some of the complex problems in the production of rubber from the guayule shrub. Without some method for determining with completeness and accuracy in terms of pure caoutchouc the value of any given sample it would have been fruitless to proceed to studies of the metabolism of the plant. The equally important problems in the control of commercial operations, both in field and factory, hinged at the outset on the development of an accurate means to determine the rubber content of the plant.

Comparison of procedures for extracting free amino acids from polymorphonuclear lekocytes.

1976 ◽  
Vol 22 (10) ◽  
pp. 1618-1622 ◽  
Author(s):  
Y Houpert ◽  
P Tarallo ◽  
G Siest
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Quantitative Determination ◽  
Free Amino Acids ◽  
Free Amino ◽  
Polymorphonuclear Leukocytes ◽  
Extraction Procedure ◽  
Intracellular Pool ◽  
Human Polymorphonuclear Leukocytes

Abstract We studies five methods for extracting amino acids from human polymorphonuclear leukocytes. Both the use of cell lysis and of a deproteinizing agent interfere with quantitative determination of the amino acids, basic amino acids being the most sensitive to the extraction procedure. Among the methods used, disruption of the cells by freezing-thawing is the best method for extracting all the amino acids. Taurine is the only amino acid extracted in the same amount by all the methods studied, and it represents half of the intracellular pool.

Quantitative Determination of Sugar Alcohols in Dietetic Foods by Gas-Liquid Partition Chromatography (GLC). I. Analysis of Dietetic Biscuits

1966 ◽  
Vol 49 (6) ◽  
pp. 1183-1187
Author(s):  
H G Jones ◽  
D M Smith ◽  
M Sahasrabudhe
Keyword(s):  
Quantitative Determination ◽  
Extraction Procedure ◽  
Internal Standard ◽  
Partition Chromatography ◽  
Sugar Alcohols ◽  
Acetate Esters ◽  
Trimethylsilyl Ethers ◽  
The Individual

Abstract GLC has been used to quantitatively measure the amount of sugar alcohols in dietetic biscuits. The total hexitol content is estimated by analysis of the trimethylsilyl ethers (TMS) and the individual hexitols are estimated as the acetate esters. An internal standard (1,4-sorbitan) was added to the biscuit during the extraction procedure. The TMS ethers were chromatographed on an SE-30 GLC column and the acetate esters were detected on a QF-l/BDS column. Recoveries of total hexitols from biscuits baked in the laboratory were 97–100%. Analyses of various commercial biscuits were also reported.

Quantitative determination of longitudinal and transverse cross-relaxation rates

1999 ◽  
Vol 96 (9/10) ◽  
pp. 1608-1615
Author(s):  
T. E. Malliavin ◽  
H. Desvaux ◽  
M. A. Delsuc
Keyword(s):  
Quantitative Determination ◽  
Cross Relaxation ◽  
Relaxation Rates
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