Plant Tissue Analysis, Colorimetric Determination of Glucose, Fructose, and Sucrose in Plant Materials Using a Combination of Enzymatic and Chemical Methods

1964 ◽  
Vol 12 (3) ◽  
pp. 216-219 ◽  
Author(s):  
Gestur Johnson ◽  
Clyde Lambert ◽  
D. K. Johnson ◽  
S. G. Sunderwirth
1960 ◽  
Vol 40 (4) ◽  
pp. 589-595 ◽  
Author(s):  
G. M. Ward ◽  
H. B. Heeney

Thirteen Canadian laboratories collaborated in a 2-year study of methods of analysis of plant tissue. The investigation has shown that the flame photometric procedure for potassium and a modification of the chelatometric method using EDTA for calcium and magnesium give the most consistent and reproducible results. Further research is necessary before the flame photometer can be used for the reliable determination of calcium and magnesium.


2021 ◽  
Author(s):  
Lynn Doran ◽  
Amanda P. De Souza

Total starch was previously extracted from plant tissue and enzymatically digested to D-Glucose. This protocol is based on the NZYtech GOD-POD kit's colorimetric determination of D-Glucose and includes basic calculations to convert D-Glucose back to total starch in the original tissue.


1935 ◽  
Vol 10 (2) ◽  
pp. 325-340 ◽  
Author(s):  
Walter C. Russell ◽  
M. W. Taylor ◽  
D. F. Chichester

1979 ◽  
Vol 62 (5) ◽  
pp. 1160-1161
Author(s):  
Krishan L Bajaj ◽  
Yogesh K Arora

Abstract A new colorimetric method is described for determining caffeic acid content in plant materials. Caffeic acid is separated by thin layer chromatography from the alcoholic extract, and color is developed using 0.5% aqueous thiosemicarbazide solution under alkaline conditions. The absorbance is read at 475 nm. Lambert-Beer's law is obeyed in the concentration range 0.37-17.5 μg caffeic acid/mL. The method is reproducible and has been applied to the estimation of caffeic acid in carrot roots.


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