Identification in Rat Plasma and Urine by Linear Trap Quadrupole–Orbitrap Mass Spectrometry of the Metabolites of Maslinic Acid, a Triterpene from Olives

2015 ◽  
Vol 63 (4) ◽  
pp. 1126-1132 ◽  
Author(s):  
Marta Sánchez-González ◽  
Glòria Lozano-Mena ◽  
Andrés Parra ◽  
M. Emília Juan ◽  
Joana M. Planas
Keyword(s):  
Mass Spectrometry ◽  
Rat Plasma ◽  
Maslinic Acid ◽  
Linear Trap ◽  
Orbitrap Mass Spectrometry

scholarly journals Characterization and Identification of Prenylated Flavonoids from Artocarpus heterophyllus Lam. Roots by Quadrupole Time-Of-Flight and Linear Trap Quadrupole Orbitrap Mass Spectrometry

Molecules ◽  
2019 ◽  
Vol 24 (24) ◽  
pp. 4591 ◽  
Author(s):  
Jin-Bao Ye ◽  
Gang Ren ◽  
Wen-Yan Li ◽  
Guo-Yue Zhong ◽  
Min Zhang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Structural Information ◽  
Structural Characteristics ◽  
Time Of Flight ◽  
Artocarpus Heterophyllus ◽  
Prenylated Flavonoids ◽  
Linear Trap ◽  
Orbitrap Mass Spectrometry ◽  
Group A ◽  
Successive Loss

In this study, a combination of quadrupole time-of-flight mass spectrometry (Q-TOF-MS) and linear trap quadrupole orbitrap mass spectrometry (LTQ-Orbitrap-MS) was performed to investigate the fragmentation behaviors of prenylated flavonoids (PFs) from Artocarpus plants. Fifteen PFs were selected as the model molecules and divided into five types (groups A–E) according to their structural characteristics in terms of the position and existing form of prenyl substitution in the flavone skeleton. The LTQ-Orbitrap-MSn spectra of the [M − H]− ions for these compounds provided a wealth of structural information on the five different types of compounds. The main fragmentation pathways of group A were the ortho effect and retro Diels–Alder (RDA), and common losses of C4H10, CO, and CO2. The compounds in group B easily lose C6H12, forming a stable structure of a 1,4-dienyl group, unlike those in group A. The fragmentation pathway for group C is characterized by obvious 1,4A−, 1,4B− cracking of the C ring. The diagnostic fragmentation for group D is obvious RDA cracking of the C ring and the successive loss of CH3 and H2O in the LTQ-Orbitrap-MSn spectra. Fragmentation with successive loss of CO or CO2, ·CH3, and CH4 in the LTQ-Orbitrap-MSn spectra formed the characteristics of group E. The summarized fragmentation rules were successfully exploited to identify PFs from Artocarpus heterophyllus, a well-known Artocarpus plant, which led to the identification of a total of 47 PFs in this plant.

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