Impact of Dietary Protein Level and Source of Polyunsaturated Fatty Acids on Lipid Metabolism-Related Protein Expression and Fatty Acid Concentrations in Porcine Tissues

2014 ◽  
Vol 62 (51) ◽  
pp. 12453-12461 ◽  
Author(s):  
Dirk Dannenberger ◽  
Karin Nuernberg ◽  
Gerd Nuernberg ◽  
Antje Priepke
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Lipid Metabolism ◽  
Protein Expression ◽  
Polyunsaturated Fatty Acids ◽  
Dietary Protein ◽  
Protein Level ◽  
Related Protein ◽  
Dietary Protein Level ◽  
Porcine Tissues

Effect of dietary fish oil on n-3 fatty acid content of meat from broiler chickens fed low protein diets

2012 ◽  
Vol 52 (9) ◽  
pp. 799 ◽  
Author(s):  
Bahman Navidshad ◽  
Liang Juan Boo ◽  
Amir Akhlaghi
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Fish Oil ◽  
Dietary Protein ◽  
Protein Level ◽  
Broiler Chickens ◽  
Dietary Protein Level ◽  
Low Protein ◽  
Dietary Fish ◽  
Protein Diets

An experiment was carried out to evaluate the effects of dietary fish oil and crude protein (CP) levels on fatty acid composition of the thigh and breast tissues of broiler chickens. Four hundred and fifty, 1-day-old mixed-sex broiler chicks were used in a completely randomised design with factorial arrangement consisting of two dietary CP levels (21 v. 18.0% and 19 v. 17.1% for grower and finisher diets, respectively), and three levels of fish oil inclusion (0, 2 or 4%). Specific increases in eicosapentaenoic acid (EPA, 20 : 5) and docosahexaenoic acid (DHA, 22 : 6) were observed in breast and thigh tissues as a response to increased fish oil supplementation. The n-6 : n-3 fatty acid ratio in breast and thigh meat samples decreased (P < 0.05) in birds fed low protein diets, but dietary protein level led to no alteration in the total n-3 fatty acids of the tissues (P > 0.05). Thigh tissue of chickens fed low protein diets had a higher concentration of DHA (P < 0.05), while the DHA and EPA concentrations in breast tissue were not affected by dietary protein level (P > 0.05). Results of the present study showed that fish oil can be used to fortify EPA and DHA levels in chicken meat and there is an interaction between dietary fatty acids and protein level on meat oxidative stability, and a reduction in dietary protein level may lead to a better oxidative stability of chicken meat.

scholarly journals Dietary Protein Level Interacts With  -3 Polyunsaturated Fatty Acid Deficiency to Induce Hypertension

2010 ◽  
Vol 23 (2) ◽  
pp. 125-128 ◽  
Author(s):  
D. P. Begg ◽  
A. J. Sinclair ◽  
L. A. Stahl ◽  
M. L. Garg ◽  
M. Jois ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Polyunsaturated Fatty Acid ◽  
Dietary Protein ◽  
Protein Level ◽  
Dietary Protein Level ◽  
Acid Deficiency ◽  
Fatty Acid Deficiency

scholarly journals Arginine supplementation modulates pig plasma lipids, but not hepatic fatty acids, depending on dietary protein level with or without leucine

2017 ◽  
Vol 13 (1) ◽  
Author(s):  
Marta Sofia Morgado dos Santos Madeira ◽  
Eva Sofia Alves Rolo ◽  
Virgínia Maria Rico Pires ◽  
Cristina Maria Riscado Pereira Mate Alfaia ◽  
Diogo Francisco Maurício Coelho ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Dietary Protein ◽  
Protein Level ◽  
Plasma Lipids ◽  
Dietary Protein Level ◽  
Arginine Supplementation

scholarly journals Adipose differentiation-related protein regulates lipids and insulin in pancreatic islets

2010 ◽  
Vol 299 (2) ◽  
pp. E249-E257 ◽  
Author(s):  
D. M. Faleck ◽  
K. Ali ◽  
R. Roat ◽  
M. J. Graham ◽  
R. M. Crooke ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Insulin Secretion ◽  
Fatty Acid ◽  
Lipid Metabolism ◽  
Acid Uptake ◽  
Mrna Levels ◽  
Related Protein ◽  
Β Cells ◽  
Adipose Differentiation ◽  
Increase Insulin Secretion

The excess accumulation of lipids in islets is thought to contribute to the development of diabetes in obesity by impairing β-cell function. However, lipids also serve a nutrient function in islets, and fatty acids acutely increase insulin secretion. A better understanding of lipid metabolism in islets will shed light on complex effects of lipids on β-cells. Adipose differentiation-related protein (ADFP) is localized on the surface of lipid droplets in a wide range of cells and plays an important role in intracellular lipid metabolism. We found that ADFP was highly expressed in murine β-cells. Moreover, islet ADFP was increased in mice on a high-fat diet (3.5-fold of control) and after fasting (2.5-fold of control), revealing dynamic changes in ADFP in response to metabolic cues. ADFP expression was also increased by addition of fatty acids in human islets. The downregulation of ADFP in MIN6 cells by antisense oligonucleotide (ASO) suppressed the accumulation of triglycerides upon fatty acid loading (56% of control) along with a reduction in the mRNA levels of lipogenic genes such as diacylglycerol O-acyltransferase-2 and fatty acid synthase. Fatty acid uptake, oxidation, and lipolysis were also reduced by downregulation of ADFP. Moreover, the reduction of ADFP impaired the ability of palmitate to increase insulin secretion. These findings demonstrate that ADFP is important in regulation of lipid metabolism and insulin secretion in β-cells.

scholarly journals Effect of polyunsaturated fatty acids on the expression of transcription factor adipocyte determination and differentiation-dependent factor 1 and of lipogenic and fatty acid oxidation enzymes in porcine differentiating adipocytes

2003 ◽  
Vol 90 (3) ◽  
pp. 507-513 ◽  
Author(s):  
J. M. Hsu ◽  
S. T. Ding
Keyword(s):  
Fatty Acids ◽  
Transcription Factor ◽  
Fatty Acid ◽  
Lipid Metabolism ◽  
Polyunsaturated Fatty Acids ◽  
Fatty Acid Synthase ◽  
Acid Oxidation ◽  
Fas Mrna ◽  
Porcine Adipocytes ◽  
Dependent Factor

Polyunsaturated fatty acids (FA) regulate genes involved in lipid metabolism. The effects of polyunsaturated FA on the transcription factor adipocyte determination and differentiation-dependent factor (ADD) 1 and fatty acid synthase (FAS) mRNA in differentiating porcine adipocytes were measured using a stromal vascular cell culture system. Porcine stromal vascular cells were isolated from subcutaneous adi-pose tissues and plated in Dulbecco's modified Eagle's medium (DMEM)–nutrient mixture F-12 Ham (F-12) plus fetal bovine serum (100 ml/l) for 24 h. Then cells were differentiated in DMEM–F12 plus insulin, hydrocortisone and transferrin without or with polyunsaturated FA at 6·25, 25·00 or 100·00 μm. The ADD1 mRNA was decreased by 100·00 μm-arachidonic acid, 6·25 to 100·00 μm-docosahexaenoic acid or cis-9,trans-11-conjugated linoleic acid. The polyunsaturated FA reduced the transcription rate of FAS, but not of ADD1. All three polyunsaturated FA accelerated degradation of ADD1 and FAS mRNA to reduce the abundance of ADD1 and FAS mRNA. Results also showed that polyunsaturated FA inhibit the ADD1 expression, not only of mRNA concentration, but also of mature ADD1 protein concentration, suggesting an overall reduction of ADD1 function by polyunsaturated FA. Our present experiments demonstrate that polyunsaturated FA regulate the gene expression of ADD1 and enzymes involved in lipid metabolism in porcine adipocytes.

scholarly journals Effect of Dietary Protein Level on Lipid Metabolism in Growing Chicks

1979 ◽  
Vol 50 (1) ◽  
pp. 44-53
Author(s):  
Keiichi TANAKA ◽  
Kazushige KITAHARA ◽  
Kakichi SHIGENO
Keyword(s):  
Lipid Metabolism ◽  
Dietary Protein ◽  
Protein Level ◽  
Dietary Protein Level

Effect of dietary protein level and conjugated linoleic acid supply on milk secretion and fecal excretion of fatty acids

2018 ◽  
Vol 245 ◽  
pp. 10-19 ◽  
Author(s):  
Stefano Schiavon ◽  
Franco Tagliapietra ◽  
Sara Pegolo ◽  
Giacomo Cesaro ◽  
Alessio Cecchinato ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Dietary Protein ◽  
Protein Level ◽  
Fecal Excretion ◽  
Milk Secretion ◽  
Dietary Protein Level
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