Evaluating Two-Dimensional Electrophoresis Profiles of the Protein Phaseolin as Markers of Genetic Differentiation and Seed Protein Quality in Common Bean (Phaseolus vulgaris L.)

2014 ◽  
Vol 62 (29) ◽  
pp. 7200-7208 ◽  
Author(s):  
María López-Pedrouso ◽  
Javier Bernal ◽  
Daniel Franco ◽  
Carlos Zapata
Keyword(s):  
Phaseolus Vulgaris ◽  
Genetic Differentiation ◽  
Common Bean ◽  
Seed Protein ◽  
Protein Quality ◽  
Two Dimensional ◽  
Phaseolus Vulgaris L ◽  
Two Dimensional Electrophoresis ◽  
Dimensional Electrophoresis

Assessment of the protein quality of fourteen soybean [Glycine max (L.) Merr.] cultivars using amino acid analysis and two-dimensional electrophoresis

2007 ◽  
Vol 40 (1) ◽  
pp. 129-146 ◽  
Author(s):  
Constantinos G. Zarkadas ◽  
Christine Gagnon ◽  
Stephen Gleddie ◽  
Shahrokh Khanizadeh ◽  
Elroy R. Cober ◽  
...  
Keyword(s):  
Glycine Max ◽  
Amino Acid ◽  
Amino Acid Analysis ◽  
Protein Quality ◽  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Glycine Max L ◽  
Dimensional Electrophoresis

Population structure and genetic differentiation among the USDA common bean (Phaseolus vulgaris L.) core collection

2011 ◽  
Vol 59 (4) ◽  
pp. 499-515 ◽  
Author(s):  
Phillip E. McClean ◽  
Jeff Terpstra ◽  
Melody McConnell ◽  
Caleb White ◽  
Rian Lee ◽  
...  
Keyword(s):  
Population Structure ◽  
Phaseolus Vulgaris ◽  
Genetic Differentiation ◽  
Common Bean ◽  
Core Collection ◽  
Phaseolus Vulgaris L

scholarly journals Genetic Diversity and Origin of Slovene Common Bean (Phaseolus vulgaris L.) Germplasm as Revealed by AFLP Markers and Phaseolin Analysis

2006 ◽  
Vol 131 (2) ◽  
pp. 242-249 ◽  
Author(s):  
Jelka Šustar-Vozlič ◽  
Marko Maras ◽  
Branka Javornik ◽  
Vladimir Meglič
Keyword(s):  
Genetic Diversity ◽  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Seed Protein ◽  
Fragment Length Polymorphism ◽  
Aflp Markers ◽  
Group Method ◽  
Phaseolus Vulgaris L ◽  
Pair Group ◽  
High Level

There is a long tradition of common bean cultivation in Slovenia, which has resulted in the development of numerous landraces in addition to newly established cultivars. The genetic diversity of 100 accessions from the Genebank of the Agricultural Institute of Slovenia (AIS) were evaluated with amplified fragment length polymorphism (AFLP) markers and phaseolin seed protein. Twenty-seven standard accessions of known Mesoamerican and Andean origin, 10 wild Phaseolus vulgaris accessions and two related species, P. coccineus L. and P. lunatus L., were also included. Ten AFLP primer combinations produced 303 polymorphic bands, indicating a relatively high level of genetic diversity. Based on the marker data, unweighted pair group method with arithmethic mean (UPGMA) analysis and principal coordinate analysis (PCoA) all P. vulgaris accessions were separated into three well-defined groups. Two groups consisted of accessions of Mesoamerican and Andean origin, while the third was comprised of only four wild P. vulgaris accessions. A set of Slovene accessions formed a well-defined sub-group within the Andean cluster, showing their unique genetic structure. These data were supported by phaseolin analysis, which also revealed additional variants of “C” and “T” phaseolin types. The results are in agreement with previous findings concerning diversification of common bean germplasm introduced in Europe.

scholarly journals Advances in the Biology of Seed and Vegetative Storage Proteins Based on Two-Dimensional Electrophoresis Coupled to Mass Spectrometry

Molecules ◽  
2018 ◽  
Vol 23 (10) ◽  
pp. 2462 ◽  
Author(s):  
Daniel Mouzo ◽  
Javier Bernal ◽  
María López-Pedrouso ◽  
Daniel Franco ◽  
Carlos Zapata
Keyword(s):  
Mass Spectrometry ◽  
Seed Development ◽  
Protein Quality ◽  
Storage Proteins ◽  
Seed Storage Proteins ◽  
Gene Families ◽  
Human Consumption ◽  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Dimensional Electrophoresis

Seed storage proteins play a fundamental role in plant reproduction and human nutrition. They accumulate during seed development as reserve material for germination and seedling growth and are a major source of dietary protein for human consumption. Storage proteins encompass multiple isoforms encoded by multi-gene families that undergo abundant glycosylations and phosphorylations. Two-dimensional electrophoresis (2-DE) is a proteomic tool especially suitable for the characterization of storage proteins because of their peculiar characteristics. In particular, storage proteins are soluble multimeric proteins highly represented in the seed proteome that contain polypeptides of molecular mass between 10 and 130 kDa. In addition, high-resolution profiles can be achieved by applying targeted 2-DE protocols. 2-DE coupled with mass spectrometry (MS) has traditionally been the methodology of choice in numerous studies on the biology of storage proteins in a wide diversity of plants. 2-DE-based reference maps have decisively contributed to the current state of our knowledge about storage proteins in multiple key aspects, including identification of isoforms and quantification of their relative abundance, identification of phosphorylated isoforms and assessment of their phosphorylation status, and dynamic changes of isoforms during seed development and germination both qualitatively and quantitatively. These advances have translated into relevant information about meaningful traits in seed breeding such as protein quality, longevity, gluten and allergen content, stress response and antifungal, antibacterial, and insect susceptibility. This review addresses progress on the biology of storage proteins and application areas in seed breeding using 2-DE-based maps.

scholarly journals Linkage disequilibrium at the APA insecticidal seed protein locus of common bean (Phaseolus vulgaris L.)

2010 ◽  
Vol 10 (1) ◽  
pp. 79 ◽  
Author(s):  
Matthew W Blair ◽  
Sergio Prieto ◽  
Lucy M Díaz ◽  
Héctor F Buendía ◽  
César Cardona
Keyword(s):  
Linkage Disequilibrium ◽  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Seed Protein ◽  
Phaseolus Vulgaris L ◽  
Protein Locus

Sialic Acid Dependent Polypeptide Chain Heterogeneity of Human Fibrinogen Demonstrated by Two-Dimensional Electrophoresis

1982 ◽  
Vol 47 (01) ◽  
pp. 019-021 ◽  
Author(s):  
Cemal Kuyas ◽  
André Haeberli ◽  
P Werner Straub
Keyword(s):  
Sialic Acid ◽  
Polypeptide Chain ◽  
Two Dimensional ◽  
Charge Heterogeneity ◽  
Human Fibrinogen ◽  
Two Dimensional Electrophoresis ◽  
Isoelectric Points ◽  
Polypeptide Chains ◽  
Dimensional Electrophoresis ◽  
Chain Type

SummaryHuman fibrinogen was compared with asialofibrinogen by two-dimensional electrophoresis to evaluate the contribution of sialic acid to the heterogeneity of the γ- and Bβ-polypeptide chains.Reduced fibrinogen showed three major variants for both the γ- and Bβ-chains. In addition two minor γ-bands with a more acidic isoelectric point than the normal γ-chains were observed. Electrophoresis in the second dimension (SDS) suggests that these most acidic bands are γ-chain-variants with a higher molecular weight. In asialofibrinogen only two predominant variants with more alkaline isoelectric points were present in each chain type.It is concluded that enzymatic removal of sialic acid partially reduces the heterogeneity of the γ- and Bβ-polypeptide chains of human fibrinogen, but additional sources producing charge heterogeneity must be sought.

Improvement of Two-dimensional Electrophoresis of Rice (Oryza sativaL.) Proteomics

2012 ◽  
Vol 18 (5) ◽  
pp. 819 ◽  
Author(s):  
Yanhua YANG ◽  
Weitong CUI ◽  
Xiaoyong LIU ◽  
Keming ZHU ◽  
Keping CHEN
Keyword(s):  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Dimensional Electrophoresis
Sign in / Sign up

Export Citation Format

Share Document