Human Metabolic Transformation of Quercetin Blocks Its Capacity To Decrease Endothelial Nitric Oxide Synthase (eNOS) Expression and Endothelin-1 Secretion by Human Endothelial Cells

2013 ◽  
Vol 61 (36) ◽  
pp. 8589-8596 ◽  
Author(s):  
Sandra Tribolo ◽  
Federica Lodi ◽  
Mark S. Winterbone ◽  
Shika Saha ◽  
Paul W. Needs ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cells ◽  
Nitric Oxide Synthase ◽  
Endothelial Nitric Oxide Synthase ◽  
Human Endothelial Cells ◽  
Enos Expression ◽  
Endothelin 1 ◽  
Metabolic Transformation ◽  
Oxide Synthase ◽  
Endothelial Nitric Oxide

scholarly journals Phytoestrogenic Effects of Blackcurrant Anthocyanins Increased Endothelial Nitric Oxide Synthase (eNOS) Expression in Human Endothelial Cells and Ovariectomized Rats

Molecules ◽  
2019 ◽  
Vol 24 (7) ◽  
pp. 1259 ◽  
Author(s):  
Kayo Horie ◽  
Naoki Nanashima ◽  
Hayato Maeda
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cells ◽  
Endothelial Nitric Oxide Synthase ◽  
Mrna Levels ◽  
Human Endothelial Cells ◽  
Enos Expression ◽  
Ovx Rats ◽  
Enos Mrna ◽  
Oxide Synthase ◽  
Endothelial Nitric Oxide

Phytoestrogens are plant-derived chemicals that are found in many foods and have estrogenic activity. We previously showed that blackcurrant extract (BCE) and anthocyanins have phytoestrogenic activity mediated via estrogen receptors (ERs), and anthocyanins may improve vascular function. BCE contains high levels of anthocyanins, but their health-promoting effects are unclear. This study examined the effects of BCE on the regulation of endothelial nitric oxide synthase (eNOS) expression and nitric oxide (NO) synthesis in human endothelial cells as key regulators in cardiovascular disease. The results showed that eNOS mRNA levels were significantly upregulated in BCE- or anthocyanin-treated human vascular endothelial cells but decreased in cells treated with fulvestrant, an ER antagonist. These results corresponded with NO levels, suggesting that BCE and anthocyanin may regulate NO synthesis via eNOS expression. Thus, the phytoestrogenic effects exerted by BCE via ERs influenced eNOS mRNA expression and NO synthesis. In vivo, we investigated whether anthocyanin-rich BCE upregulated eNOS protein expression in ovariectomized (OVX) rats, a widely used animal model of menopause. Our results showed that anthocyanin-rich BCE significantly upregulated eNOS mRNA levels and NO synthesis through phytoestrogenic activity and therefore promoted blood vessel health in OVX rats as a postmenopausal model.

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