Improved Physical and in Vitro Digestion Stability of a Polyelectrolyte Delivery System Based on Layer-by-Layer Self-Assembly Alginate–Chitosan-Coated Nanoliposomes

2013 ◽  
Vol 61 (17) ◽  
pp. 4133-4144 ◽  
Author(s):  
Weilin Liu ◽  
Jianhua Liu ◽  
Wei Liu ◽  
Ti Li ◽  
Chengmei Liu
Keyword(s):  
Delivery System ◽  
Self Assembly ◽  
In Vitro Digestion ◽  
Layer By Layer

A New Recombinant Fibronectin/Cadherin Protein-Hydrophobically Modified Glycol Chitosan/Paclitaxel Layer-By-Layer Self-Assembly Strategy for the Postoperative Therapy of Osteosarcoma and Correlated Bone Injury

2021 ◽  
Vol 17 (9) ◽  
pp. 1765-1777
Author(s):  
Zaiyang Liu ◽  
Yiqun Wu ◽  
Hongjuan Dai ◽  
Shasha Li ◽  
Ying Zhu ◽  
...  
Keyword(s):  
Fusion Protein ◽  
Tumor Cells ◽  
Self Assembly ◽  
Blood Circulation ◽  
Residual Tumor ◽  
Layer By Layer ◽  
Glycol Chitosan ◽  
Hydrophobically Modified ◽  
Bone Injury

Osteosarcoma is one of the most aggressive cancers which greatly threatens the health of adolescents and surgery is difficult to resect the whole piece of tumor tissue. The residual tumor cells might proliferate at the tumor site and invade into the blood circulation, leading to tumor recurrence and metastasis. Besides, the invasion of tumor cells could also lead to bone injury. We designed a recombinant fibronectin-cadherin fusion protein/hydrophobically modified glycol chitosan-PTX nanoparticles (rFN-CDH/HGC-PTX) layer-by-layer self-assembly polymer based on biphasic calcium phosphate ceramic (BCP) (BCP-PEI-(rFN/CDH-PTX/HGC)n-rFN/CDH). The SEM, FTIR, XPS and contact angle experiments proved the successful synthesis of the polymer. The chemotherapy drug PTX and bone-repairing-related rFN/CDH fusion protein could be stably released within one week and the in vitro experiments exhibited the efficacy of the polymer to kill residual tumor cells and promote the proliferation of osteoblast, confirming that our polymer was a superior material for postoperative osteosarcoma therapy.

scholarly journals Fabrication and in vitro evaluation of stable collagen/hyaluronic acid biomimetic multilayer on titanium coatings

2013 ◽  
Vol 10 (84) ◽  
pp. 20130070 ◽  
Author(s):  
Haiyong Ao ◽  
Youtao Xie ◽  
Honglue Tan ◽  
Shengbing Yang ◽  
Kai Li ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Photoelectron Spectroscopy ◽  
Self Assembly ◽  
Human Mesenchymal Stem Cells ◽  
Biological Properties ◽  
Covalent Immobilization ◽  
Layer By Layer ◽  
Titanium Coating ◽  
Assembly Technique

Layer-by-layer (LBL) self-assembly technique has been proved to be a highly effective method to immobilize the main components of the extracellular matrix such as collagen and hyaluronic acid on titanium-based implants and form a polyelectrolyte multilayer (PEM) film by electrostatic interaction. However, the formed PEM film is unstable in the physiological environment and affects the long-time effectiveness of PEM film. In this study, a modified LBL technology has been developed to fabricate a stable collagen/hyaluronic acid (Col/HA) PEM film on titanium coating (TC) by introducing covalent immobilization. Scanning electron microscopy, diffuse reflectance Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy were used to characterize the PEM film. Results of Sirius red staining demonstrated that the chemical stability of PEM film was greatly improved by covalent cross-linking. Cell culture assays further illustrated that the functions of human mesenchymal stem cells, such as attachment, spreading, proliferation and differentiation, were obviously enhanced by the covalently immobilized Col/HA PEM on TCs compared with the absorbed Col/HA PEM. The improved stability and biological properties of the Col/HA PEM covalently immobilized TC may be beneficial to the early osseointegration of the implants.

scholarly journals Targeted Co-Delivery of siRNA and Methotrexate for Tumor Therapy via Mixed Micelles

Pharmaceutics ◽  
2019 ◽  
Vol 11 (2) ◽  
pp. 92 ◽  
Author(s):  
Fei Hao ◽  
Robert Lee ◽  
Chunmiao Yang ◽  
Lihuang Zhong ◽  
Yating Sun ◽  
...  
Keyword(s):  
Delivery System ◽  
Self Assembly ◽  
Target Gene ◽  
Mixed Micelles ◽  
Folate Receptor ◽  
Tumor Therapy ◽  
Narrow Particle Size Distribution ◽  
Combination Drug ◽  
Effective Carrier

A combination of chemotherapeutic drugs and siRNA is emerging as a new modality for cancer therapy. A safe and effective carrier platform is needed for combination drug delivery. Here, a functionalized mixed micelle-based delivery system was developed for targeted co-delivery of methotrexate (MTX) and survivin siRNA. Linolenic acid (LA) was separately conjugated to branched polyethlenimine (b-PEI) and methoxy-polyethyleneglycol (mPEG). MTX was then conjugated to LA-modified b-PEI (MTX-bPEI-LA) to form a functionalized polymer-drug conjugate. Functionalized mixed micelles (M-MTX) were obtained by the self-assembly of MTX-bPEI-LA and LA-modified mPEG (mPEG-LA). M-MTX had a narrow particle size distribution and could successfully condense siRNA at an N/P ratio of 16/1. M-MTX/siRNA was selectively taken up by HeLa cells overexpressing the folate receptor (FR) and facilitated the release of the siRNA into the cytoplasm. In vitro, M-MTX/siRNA produced a synergy between MTX and survivin siRNA and markedly suppressed survivin protein expression. In tumor-bearing mice, M-MTX/Cy5-siRNA showed an elevated tumor uptake. In addition, M-MTX/siRNA inhibited tumor growth. Immunohistochemistry and a western blot analysis showed a significant target gene downregulation. In conclusion, M-MTX/siRNA was highly effective as a delivery system and may serve as a model for the targeted co-delivery of therapeutic agents.

scholarly journals Preparation and Characterization of Novel Perfluorooctyl Bromide Nanoparticle as Ultrasound Contrast Agent via Layer-by-Layer Self-Assembly for Folate-Receptor-Mediated Tumor Imaging

2016 ◽  
Vol 2016 ◽  
pp. 1-14 ◽  
Author(s):  
Yue Hu ◽  
Yong Wang ◽  
Jianshuai Jiang ◽  
Baosan Han ◽  
Shengmin Zhang ◽  
...  
Keyword(s):  
Ultrasound Imaging ◽  
Self Assembly ◽  
Folate Receptor ◽  
Layer By Layer ◽  
The Novel ◽  
Chitosan Derivative ◽  
Glycol Chitosan ◽  
Targeted Nanoparticles ◽  
Perfluorooctyl Bromide

A folate-polyethylene glycol-chitosan derivative was synthesized and its structure was characterized. An optimal perfluorooctyl bromide nanocore template was obtained via utilizing the ultrasonic emulsification method combining with orthogonal design. The targeted nanoparticles containing targeted shell of folate-polyethylene glycol-chitosan derivative and perfluorooctyl bromide nanocore template of ultrasound imaging were prepared successfully by exploiting layer-by-layer self-assembly as contrast agent for ultrasound. Properties of the novel perfluorooctyl bromide nanoparticle were extensively studied by Dynamic Light Scattering and Transmission Electron Microscopy. The targeted nanoparticle diameter, polydispersity, and zeta potential are around 229.5 nm, 0.205, and44.7±0.6 mV, respectively. The study revealed that spherical core-shell morphology was preserved. Excellent stability of targeted nanoparticle is evidenced by two weeks of room temperature stability tests. The results of the cell viability assay and the hemolysis test confirmed that the targeted nanoparticle has an excellent biocompatibility for using in cell studies and ultrasound imaging in vivo. Most importantly, in vitro cell experiments demonstrated that an increased amount of targeted nanoparticles was accumulated in hepatocellular carcinoma cell line Bel7402 relative to hepatoma cell line L02. And targeted nanoparticles had also shown better ultrasound imaging abilities in vitro. The data suggest that the novel targeted nanoparticle may be applicable to ultrasonic molecular imaging of folate-receptor overexpressed tumor.

Construction of Biodegradable Multilayer Films via Layer-by-Layer Self-Assembly as Gene Delivery System

2005 ◽  
Vol 288-289 ◽  
pp. 101-104 ◽  
Author(s):  
Ke Feng Ren ◽  
Jian Ji ◽  
Jia Cong Shen
Keyword(s):  
Self Assembly ◽  
Multilayer Films ◽  
Layer By Layer ◽  
The Novel ◽  
Gene Delivery System ◽  
Layer Method ◽  
Uv Visible ◽  
Spectrometry Measurement ◽  
Visible Spectrometry

Films composed of alternating layers of protamine and DNA were constructed using the layer-by-layer method on quartz and subsequently studied the enzymic degradation in vitro. UV-visible spectrometry measurement indicated the uniform assembly of Protamine/DNA multilayer films. UV-visible spectrometry and fluorescence spectrometry results revealed that the Protamine/DNA multilayer films were in vitro enzymic biodegradable. The novel biodegradable multilayer of Protamine/DNA may have great potential for gene therapy applications in tissue engineering, medical implant etc.

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