Correlation of Total Antioxidant Capacity with Reactive Oxygen Species (ROS) Consumption Measured by Oxidative Conversion

2013 ◽  
Vol 61 (22) ◽  
pp. 5260-5270 ◽  
Author(s):  
Sema Demirci Çekiç ◽  
Aydan Çetinkaya ◽  
Aslı Neslihan Avan ◽  
Reşat Apak
Keyword(s):  
Reactive Oxygen Species ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Oxidative Conversion ◽  
Total Antioxidant

Total Antioxidant Capacity and Reactive Oxygen Species in Amniotic Fluid

2003 ◽  
Vol 101 (4) ◽  
pp. 756-761 ◽  
Author(s):  
Janet M. Burlingame ◽  
Navid Esfandiari ◽  
Rakesh K. Sharma ◽  
Edward Mascha ◽  
Tommaso Falcone
Keyword(s):  
Reactive Oxygen Species ◽  
Amniotic Fluid ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Total Antioxidant

scholarly journals Electrochemical Methods for Total Antioxidant Capacity and its Main Contributors Determination: A review

2015 ◽  
Vol 13 (1) ◽  
Author(s):  
Aurelia Magdalena Pisoschi ◽  
Carmen Cimpeanu ◽  
Gabriel Predoi
Keyword(s):  
Reactive Oxygen Species ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Electrochemical Techniques ◽  
Reactive Oxygen ◽  
Electrochemical Methods ◽  
Oxygen Species ◽  
Advantages And Disadvantages ◽  
Total Antioxidant ◽  
Antioxidant Molecule

AbstractBackround: The present review focuses on electrochemical methods for antioxidant capacity and its main contributors assessment. The main reactive oxygen species, responsible for low density lipoprotein oxidation, and their reactivity are reminded. The role of antioxidants in counteracting the factors leading to oxidative stress-related degenerative diseases occurence, is then discussed. Antioxidants can scavenge free radicals, can chelate pro-oxidative metal ions, or quench singlet oxygen. When endogenous factors (uric acid, bilirubin, albumin, metallothioneins, superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, glutathione-S-transferase) cannot accomplish their protective role against reactive oxygen species, the intervention of exogenous antioxidants (vitamin C, tocopherols, flavonoids, carotenoids etc) is required, as intake from food, as nutritional supplements or as pharmaceutical products.Literature study: The main advantages of electrochemical methods with respect to traditional, more laborious instrumental techniques are described: sensitivity, rapidity, simplicity of the applied analytical procedure which does not require complicated sample pre-treatment etc.The paper reviews minutiously the voltammetric, amperometric, biamperometric, potentiometric and coulometric methods for total antioxidant capacity estimation. For each method presented, the electroactivity and the mechanism of electro-oxidation of antioxidant molecules at various electrodes, as well as the influences on the electroactive properties are discussed. The characteristics of the developed methods are viewed from the perspective of the antioxidant molecule structure influence, as well as from the importance of electrode material and/or surface groups standpoint.The antioxidant molecule-electrode surface interaction, the detection system chosen, the use of modifiers, as well as the nature of the analysed matrix are the factors discussed, which influence the performances of the studied electrochemical techniques.Conclusions: The electrochemical methods reviewed in this paper allow the successful determination of the total antioxidant capacity and of its main contributors in various media: foodstuffs and beverages, biological fluids, pharmaceuticals. The advantages and disadvantages of the electrochemical methods applied to antioxidant content and antioxidant activity assay are treated and interpreted, in the case of various analysed matrixes. Combining advanced materials with classical electrode construction, provides viable results and can constitute an alternative for the future.

scholarly journals Seminal reactive oxygen species and total antioxidant capacity: Correlations with sperm parameters and impact on male infertility

2018 ◽  
Vol 45 (2) ◽  
pp. 88-93 ◽  
Author(s):  
Vidyalakshmi Subramanian ◽  
Aishwarya Ravichandran ◽  
Nivethitha Thiagarajan ◽  
Matheswari Govindarajan ◽  
Silambuchelvi Dhandayuthapani ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Male Infertility ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Reactive Oxygen ◽  
Sperm Parameters ◽  
Oxygen Species ◽  
Total Antioxidant

scholarly journals The reactive oxygen species—total antioxidant capacity score is a new measure of oxidative stress to predict male infertility*

1999 ◽  
Vol 14 (11) ◽  
pp. 2801-2807 ◽  
Author(s):  
Rakesh K. Sharma ◽  
Fabio F. Pasqualotto ◽  
David R. Nelson ◽  
Anthony J. Thomas ◽  
Ashok Agarwal
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Male Infertility ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Total Antioxidant

scholarly journals Fluctuations in total antioxidant capacity, catalase activity and hydrogen peroxide levels of follicular fluid during bovine folliculogenesis

2011 ◽  
Vol 23 (5) ◽  
pp. 673 ◽  
Author(s):  
Sajal Gupta ◽  
Audrey Choi ◽  
Hope Y. Yu ◽  
Suzanne M. Czerniak ◽  
Emily A. Holick ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Hydrogen Peroxide ◽  
Antioxidant Capacity ◽  
Follicular Fluid ◽  
Total Antioxidant Capacity ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Total Antioxidant ◽  
Follicle Size

Follicular fluid is an important environment for oocyte development, yet current knowledge regarding its in vivo oxidant and antioxidant levels remains limited. Examining follicular fluid oxidants and antioxidants will improve understanding of their changes in vivo and contribute to optimisation of in vitro maturation conditions. The aim of the present study was to consider selected markers, namely catalase (CAT) enzyme activity, total antioxidant capacity (TAC) and hydrogen peroxide (H2O2) in follicular fluid samples (n = 503) originating from bovine antral follicles. The dynamic changes in two relevant antioxidant measures and one reactive oxygen species (ROS) were measured through stages of bovine follicular development and the oestrous cycle. CAT activity and H2O2 levels decreased significantly as follicle size increased, whereas TAC increased significantly as follicle size increased. Lower TAC and higher H2O2 in small follicles suggest increased ROS in the initial stages of folliculogenesis. Because CAT levels are highest in the follicular fluid of small follicles in the setting of an overall low TAC, CAT may represent a dominant antioxidant defence in the initial stages of folliculogenesis. Future studies must focus on other reactive oxygen species and their various scavenger types during antral folliculogenesis.

Role of reactive oxygen species–total antioxidant capacity status in Telfairia occidentalis leaves–associated spermatoprotective effect: a pointer to fatty acids benefit

2018 ◽  
Vol 29 (4) ◽  
pp. 347-358 ◽  
Author(s):  
Toyin Mohammed Salman ◽  
Isiaka Abdullateef Alagbonsi ◽  
Abdul-Rahuf Aderemi Feyitimi
Keyword(s):  
Reactive Oxygen Species ◽  
Fatty Acids ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Reactive Oxygen ◽  
Semen Parameters ◽  
Oxygen Species ◽  
Telfairia Occidentalis ◽  
Total Antioxidant

AbstractBackgroundThe present study used reactive oxygen species (ROS)–total antioxidant capacity (TAC) score to understand the role of redox status on the effect ofTelfairia occidentalis(TO) on testicular parameters. The fatty acids (FAs) components of methanol extract of TO (METO) and its fractions were also identified with gas chromatography–mass spectrometry.MethodsA total of 66 male Wistar rats were randomly divided in a blinded fashion into six oral treatment groups as follows: group I (control, n=6) received 10% ethanol (vehicle for TO administration). Groups II to VI (n=12 rats each) were subdivided into two treatment subgroups (n=6 each) that received 200 mg/kg and 600 mg/kg of METO and its chloroform, petroleum ether, acetone, and ethanol fractions, respectively. All treatments lasted for 30 days.ResultsThe major FAs detected in TO were myristic, palmitic, oleic, linoleic, linolenic, and stearic acids including their esters. Both doses of METO and its fractions increased the semen parameters, TAC and ROS-TAC scores but decreased the ROS when compared with control.ConclusionsUsing the ROS-TAC score, this study suggests that TO-associated improvement in semen parameters might be partly mediated by a reduction in free radical generation, and that the FAs present in TO might be involved in its spermatoprotective effect.

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