Development of a Locked Nucleic Acid Real-Time Polymerase Chain Reaction Assay for the Detection of Pinus armandii in Mixed Species Pine Nut Samples Associated with Dysgeusia

2013 ◽  
Vol 61 (5) ◽  
pp. 1060-1066 ◽  
Author(s):  
Sara M. Handy ◽  
Ruth E. Timme ◽  
Salena M. Jacob ◽  
Jonathan R. Deeds
Keyword(s):  
Polymerase Chain Reaction ◽  
Nucleic Acid ◽  
Real Time ◽  
Polymerase Chain Reaction Assay ◽  
Locked Nucleic Acid ◽  
Mixed Species ◽  
Chain Reaction ◽  
Pine Nut ◽  
Polymerase Chain

scholarly journals Detection of the Janus kinase 2 V617F mutation using a locked nucleic-acid, real-time polymerase chain reaction assay

2018 ◽  
Vol 7 (1) ◽  
Author(s):  
Tshiphiri Senamela ◽  
Marleen Kock ◽  
Piet Becker ◽  
Joachim J.C. Potgieter
Keyword(s):  
Polymerase Chain Reaction ◽  
Nucleic Acid ◽  
Real Time ◽  
Jak2 V617f ◽  
Locked Nucleic Acid ◽  
Jak2 V617f Mutation ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Polymerase Chain ◽  
V617f Mutation

The purpose of this study was to develop a real time polymerase chain reaction (PCR) assay for the detection of the JAK2 V617F mutation that could be used in diagnostic laboratories. Sanger sequencing and a newly developed locked nucleic-acid, real-time PCR assay were used to detect the JAK2 V617F mutation. There was 100% agreement between the sequencing and PCR analysis. Both assays were able to detect the mutation in all 24 of the 60 test specimens harbouring the mutation.

scholarly journals AB0691 ANALYSIS OF SARS-COV-2 ANTIBODIES IN NON-COVID-19 PATIENTS: COMPARISON BETWEEN SYSTEMIC SCLEROSIS PATIENTS AND HEALTHY CONTROLS

2021 ◽  
Vol 80 (Suppl 1) ◽  
pp. 1379.1-1379
Author(s):  
L. Giardullo ◽  
C. Rotondo ◽  
A. Corrado ◽  
N. Maruotti ◽  
R. Colia ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Systemic Sclerosis ◽  
Autoimmune Disease ◽  
Reverse Transcriptase ◽  
Real Time ◽  
Polymerase Chain Reaction Assay ◽  
Nuclear Antigen ◽  
Healthy Controls ◽  
Chain Reaction ◽  
Polymerase Chain

Background:Previous study evidenced a cross-reactivity between Sars-Cov-2 antibodies and autoimmune tissue antigen involved in connective tissue diseases, as nuclear antigen (NA), extractable nuclear antigen (ENA), histone and collagen (1). No study has been published about the titer of Sars-Cov-2 antibodies in non-infected patients with autoimmune disease.Objectives:To evaluate the titer of SARS-CoV-2 antibodies in non-COVID-19 patients and compare it between systemic sclerosis (SSc) patients and healthy controls (HC).Methods:A total of 58 patients with SSc (who fulfilled ACR/EULAR 2013 SSc classification criteria) and 18 HC were enrolled. Sera of all participants were collected, and SARS-CoV-2 antibodies (IgG and IgM) were evaluated by means ELISA. In all participants swabs for SARS-CoV-2 by real-time reverse-transcriptase-polymerase-chain-reaction assay were reported negative. Demographic, clinical, and autoimmune serological characteristics of SSc patients were recorded. The normal distribution was assessed using the Shapiro–Wilk’s test. Exclusion criteria was previous or actual Sars-Cov-2 infection. Comparisons between study groups of patients were evaluated by the Student’s t-test or Mann – Whitney U-test as appropriate. The differences between categorial variables were assessed by Pearson chi-square or Fisher’s exact test, as opportune. Statistical significance was set at p ≤ 0.05.Results:We observed significant differences between SSc patients and HC in serum levels of Sars-Cov-2 antibodies (IgG: 1,4±2,1 AU/ml vs 0,36±0,19 AU/ml respectively (p=0,001); and IgM: 2,5±3,1 AU/ml vs 0,8±0,7 AU/ml (p=0,022)). In 5 SSc patients was found titer of Sars-Cov-2 antibodies (IgG) exceeding the cut-off, but the control of swabs for SARS-CoV-2 by real-time reverse-transcriptase-polymerase-chain-reaction assay were negative. No significative differences in Sars-Cov-2 autoantibodies titer were found in subgroup of SSc patients with or without ILD or PAH, limited or diffuse skin subset, and different autoantibodies profile. Furthermore, antibodies titer was not associated with different drugs (steroid, methotrexate, mofetil-mycophenolate and bosentan) in use.Conclusion:A cross mimicking between Sars-Cov-2 antibodies and antinuclear antibodies or anti ENA could be hypothesized. Further studies are necessary to unravel the reliability of Sars-Cov-2 antibodies detection in autoimmune disease.References:[1]Vojdani, A., Vojdani, E., & Kharrazian, D. (2021). Reaction of human monoclonal antibodies to SARS-CoV-2 proteins with tissue antigens: Implications for autoimmune diseases. Frontiers in Immunology, 11, 3679Disclosure of Interests:None declared

scholarly journals Development of a real time polymerase chain reaction assay for equine encephalosis virus

2014 ◽  
Vol 195 ◽  
pp. 205-210 ◽  
Author(s):  
N.M. Rathogwa ◽  
M. Quan ◽  
J.Q. Smit ◽  
C. Lourens ◽  
A.J. Guthrie ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Polymerase Chain Reaction Assay ◽  
Chain Reaction ◽  
Polymerase Chain

Evaluation of cytomegalovirus infection in low-birth weight children by breast milk using a real-time polymerase chain reaction assay

2015 ◽  
Vol 87 (5) ◽  
pp. 845-850 ◽  
Author(s):  
Romero-Gómez Maria Pilar ◽  
Cabrera Marta ◽  
Montes-Bueno María Teresa ◽  
Cendejas-Bueno Emilio ◽  
Segovia Cristina ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Birth Weight ◽  
Breast Milk ◽  
Low Birth Weight ◽  
Real Time ◽  
Polymerase Chain Reaction Assay ◽  
Cytomegalovirus Infection ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Low Birth Weight Children
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