Concordance Study: Methods of Quantifying Corn and Soybean Genomic DNA Intended for Real-Time Polymerase Chain Reaction Applications

2012 ◽  
Vol 60 (34) ◽  
pp. 8323-8332 ◽  
Author(s):  
G. Ronald Jenkins ◽  
Jennifer T. Helber ◽  
Larry D. Freese
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Genomic Dna ◽  
Chain Reaction ◽  
Study Methods ◽  
Polymerase Chain

Rapid Determination of Perv Copy Number From Porcine Genomic DNA by Real-Time Polymerase Chain Reaction

2011 ◽  
Vol 22 (4) ◽  
pp. 175-180 ◽  
Author(s):  
Donghee Lee ◽  
Jungeun Lee ◽  
Jong-Kwang Yoon ◽  
Na Young Kim ◽  
Gye-Woong Kim ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Copy Number ◽  
Genomic Dna ◽  
Rapid Determination ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals A New TaqMan Real-Time Polymerase Chain Reaction Assay for Quantification of Fusarium virguliforme in Soil

Plant Disease ◽  
2011 ◽  
Vol 95 (11) ◽  
pp. 1420-1426 ◽  
Author(s):  
G. C. Y. Mbofung ◽  
A. Fessehaie ◽  
M. K. Bhattacharyya ◽  
L. F. S. Leandro
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Polymerase Chain Reaction Assay ◽  
Genomic Dna ◽  
Inoculum Density ◽  
Chain Reaction ◽  
Greenhouse Soil ◽  
Fusarium Virguliforme ◽  
Assay Sensitivity ◽  
Polymerase Chain

The quantification of the soilborne pathogen Fusarium virguliforme inoculum in soil is important for epidemiological studies of soybean sudden death syndrome (SDS). Classical dilution plating methods to determine inoculum density in soil have yielded inconsistent results due to slow growth, variable colony morphology of the pathogen, and the presence of other fungi with similar phenotype. A TaqMan real-time polymerase chain reaction assay was developed based on sequences of the FvTox1 gene of F. virguliforme. The gene differed by four single-nucleotide proteins from the other SDS-causing species. Assay specificity was tested on 48 fungal isolates that varied in taxonomic relatedness. Assay sensitivity was appraised on 10-fold serial dilutions of genomic DNA, conidia suspensions, and soil spiked with conidia. Applicability of the assay was evaluated on field and greenhouse soil samples, and on roots of symptomatic plants. The assay detected only DNA sequences specific to F. virguliforme. The detection limit of the assay was 5 pg/μl, 1,000 conidia/ml, and 1,000 conidia/g soil for genomic DNA, conidial suspensions, and soil with conidia, respectively. The assay was specific to F. virguliforme and was used successfully to quantify inoculum density in soil and soybean roots. The assay can be used as a diagnostic tool for rapid screens of field and greenhouse soil, and for symptomatic and asymptomatic plants.

Real-Time Polymerase Chain Reaction of Genomic DNA for Quantitation of t(14;18)

2003 ◽  
pp. 223-236
Author(s):  
Apostolia-Marie Tsimberidou ◽  
Yunfang Jiang ◽  
Richard J. Ford ◽  
Fernando Cabanillas ◽  
Andreas H. Sarris
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Genomic Dna ◽  
Chain Reaction ◽  
Polymerase Chain

1504: Molecular Diagnosis and Staging of Prostate Cancer Using Clusterin in Real Time Reverse Transcription Polymerase Chain Reaction (RT-PCR)

2006 ◽  
Vol 175 (4S) ◽  
pp. 485-486
Author(s):  
Sabarinath B. Nair ◽  
Christodoulos Pipinikas ◽  
Roger Kirby ◽  
Nick Carter ◽  
Christiane Fenske
Keyword(s):  
Prostate Cancer ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Molecular Diagnosis ◽  
Reverse Transcription ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain
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