Solid-State Selective13C Excitation and Spin Diffusion NMR To Resolve Spatial Dimensions in Plant Cell Walls

2012 ◽  
Vol 60 (6) ◽  
pp. 1419-1427 ◽  
Author(s):  
Marcus Foston ◽  
Rui Katahira ◽  
Erica Gjersing ◽  
Mark F. Davis ◽  
Arthur J. Ragauskas
Keyword(s):  
Solid State ◽  
Plant Cell ◽  
Cell Walls ◽  
Spin Diffusion ◽  
Plant Cell Walls ◽  
Diffusion Nmr ◽  
Spatial Dimensions

scholarly journals Sensitivity-enhanced solid-state NMR detection of expansin's target in plant cell walls

2013 ◽  
Vol 110 (41) ◽  
pp. 16444-16449 ◽  
Author(s):  
T. Wang ◽  
Y. B. Park ◽  
M. A. Caporini ◽  
M. Rosay ◽  
L. Zhong ◽  
...  
Keyword(s):  
Solid State ◽  
Plant Cell ◽  
Solid State Nmr ◽  
Cell Walls ◽  
Plant Cell Walls

scholarly journals Folding of xylan onto cellulose fibrils in plant cell walls revealed by solid-state NMR

2016 ◽  
Vol 7 (1) ◽  
Author(s):  
Thomas J. Simmons ◽  
Jenny C. Mortimer ◽  
Oigres D. Bernardinelli ◽  
Ann-Christin Pöppler ◽  
Steven P. Brown ◽  
...  
Keyword(s):  
Solid State ◽  
Plant Cell ◽  
Solid State Nmr ◽  
Cell Walls ◽  
Plant Cell Walls ◽  
Cellulose Fibrils

scholarly journals 11B Solid-state NMR Investigation of the Rhamnogalacturonan II-borate Complex in Plant Cell Walls

2006 ◽  
Vol 22 (2) ◽  
pp. 321-323 ◽  
Author(s):  
Tsunenori KAMEDA ◽  
Tadashi ISHII ◽  
Toshiro MATSUNAGA ◽  
Jun ASHIDA
Keyword(s):  
Solid State ◽  
Plant Cell ◽  
Solid State Nmr ◽  
Cell Walls ◽  
Plant Cell Walls ◽  
Borate Complex ◽  
Rhamnogalacturonan Ii

scholarly journals Solid-state NMR of unlabeled plant cell walls: high-resolution structural analysis without isotopic enrichment

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Wancheng Zhao ◽  
Alex Kirui ◽  
Fabien Deligey ◽  
Frederic Mentink-Vigier ◽  
Yihua Zhou ◽  
...  
Keyword(s):  
Solid State ◽  
Plant Cell ◽  
Cell Walls ◽  
Nmr Relaxation ◽  
Isotopic Labeling ◽  
Plant Cell Walls ◽  
Cell Wall Assembly ◽  
Dipolar Order ◽  
Wall Assembly ◽  
Polymorphic Structure

Abstract Background Multidimensional solid-state nuclear magnetic resonance (ssNMR) spectroscopy has emerged as an indispensable technique for resolving polymer structure and intermolecular packing in primary and secondary plant cell walls. Isotope (13C) enrichment provides feasible sensitivity for measuring 2D/3D correlation spectra, but this time-consuming procedure and its associated expenses have restricted the application of ssNMR in lignocellulose analysis. Results Here, we present a method that relies on the sensitivity-enhancing technique Dynamic Nuclear Polarization (DNP) to eliminate the need for 13C-labeling. With a 26-fold sensitivity enhancement, a series of 2D 13C–13C correlation spectra were successfully collected using the unlabeled stems of wild-type Oryza sativa (rice). The atomic resolution allows us to observe a large number of intramolecular cross peaks for fully revealing the polymorphic structure of cellulose and xylan. NMR relaxation and dipolar order parameters further suggest a sophisticated change of molecular motions in a ctl1 ctl2 double mutant: both cellulose and xylan have become more dynamic on the nanosecond and microsecond timescale, but the motional amplitudes are uniformly small for both polysaccharides. Conclusions By skipping isotopic labeling, the DNP strategy demonstrated here is universally extendable to all lignocellulose materials. This time-efficient method has landed the technical foundation for understanding polysaccharide structure and cell wall assembly in a large variety of plant tissues and species.

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