Cellulase Hydrolysis of Rice Straw and Inactivation of Endoglucanase in Urea Solution

2011 ◽  
Vol 59 (20) ◽  
pp. 10971-10975 ◽  
Author(s):  
Yi-Ming Tao ◽  
Xin-Qi Xu ◽  
Su-Juan Ma ◽  
Ge Liang ◽  
Xiao-Bing Wu ◽  
...  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Chiranjeevi Thulluri ◽  
Ravi Balasubramaniam ◽  
Harshad Ravindra Velankar

AbstractCellulolytic enzymes can readily access the cellulosic component of lignocellulosic biomass after the removal of lignin during biomass pretreatment. The enzymatic hydrolysis of cellulose is necessary for generating monomeric sugars, which are then fermented into ethanol. In our study, a combination of a deep eutectic (DE) mixture (of 2-aminoethanol and tetra-n-butyl ammonium bromide) and a cyclic ether (tetrahydrofuran) was used for selective delignification of rice straw (RS) under mild conditions (100 °C). Pretreatment with DE-THF solvent system caused ~ 46% delignification whereas cellulose (~ 91%) and hemicellulose (~ 67%) recoveries remained higher. The new solvent system could be reused upto 10 subsequent cycles with the same effectivity. Interestingly, the DE-THF pretreated cellulose showed remarkable enzymatic hydrolysability, despite an increase in its crystallinity to 72.3%. Contrary to conventional pretreatments, we report for the first time that the enzymatic hydrolysis of pretreated cellulose is enhanced by the removal of lignin during DE-THF pretreatment, notwithstanding an increase in its crystallinity. The current study paves way for the development of newer strategies for biomass depolymerization with DES based solvents.


2003 ◽  
Vol 105 (1-3) ◽  
pp. 155-164 ◽  
Author(s):  
Betzabé Sulbarán-De-Ferrer ◽  
Marielena Aristiguieta ◽  
Bruce E. Dale ◽  
Alexis Ferrer ◽  
Graciela Ojeda-De-Rodriguez

2017 ◽  
Vol 20 (2) ◽  
pp. 1364-1370 ◽  
Author(s):  
Jinyang Chen ◽  
Chao Zhang ◽  
Mingli Li ◽  
Jingmin Chen ◽  
Yingdi Wang ◽  
...  

2012 ◽  
Vol 123 ◽  
pp. 636-645 ◽  
Author(s):  
Kyoung-Mi Lee ◽  
Dayanand Kalyani ◽  
Manish Kumar Tiwari ◽  
Tae-Su Kim ◽  
Saurabh Sudha Dhiman ◽  
...  

Sen-ito Kogyo ◽  
1971 ◽  
Vol 4 (1) ◽  
pp. 22-27
Author(s):  
YOSHITAKA OGIWARA ◽  
KENICHIRO ARAI

Author(s):  
Jaruwan Damaurai ◽  
Thanchanok Preechakun ◽  
Marisa Raita ◽  
Verawat Champreda ◽  
Navadol Laosiripojana

2014 ◽  
Vol 27 (2) ◽  
pp. 20-24
Author(s):  
Pradip Saha ◽  
MR Khan ◽  
TK Deb ◽  
S Majumdar ◽  
F Alam ◽  
...  

As the gradual up-growing trend of industrialization and urbanization leading steady increment of demand of energy; eco-friendly, bio degradable, cost competitive and promising source of energy with high sustainability is toughly needed for the new era of modern world. Hydrolysis of cellulose by cellulase enzymes is a vital candidate for this option. It is a solid-liquid heterogeneous reaction; strongly affected by the non-reaction resistances caused most notably by the crystalline structure; reaction environment parameters as temperature, pH, characteristics of enzyme, cell & substrate loading and hence must have to be defined for specific enzyme-substrate amalgamation. In this present investigation, glucose was produced from rice straw using cellulytic enzyme pseudomonas sp., isolated from municipal solid waste. Glucose yield was found to increase as the rice straw particle size decreased from 0.5 mm to 45 ?m, while the optimal temperature and pH were found within the range of 30°C and 7.0 respectively. The concentration and rate of glucose production was observed to depend on pretreatment of rice straw, substrate concentration and enzyme loading. A kinetic model rate expression has been developed for such a process based on the Michaelis – Mentens and Line weaver–Burk approach. Comparison between the experimental data and those predicted from the rate model indicate good agreement with a mean absolute deviation of about 0.304916. DOI: http://dx.doi.org/10.3329/jce.v27i2.17778 Journal of Chemical Engineering, IEB Vol. ChE. 27, No. 2, December 2012: 20-24


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