Enzyme-Linked Immunosorbent Assay with Monoclonal and Single-Chain Variable Fragment Antibodies Selective to Coplanar Polychlorinated Biphenyls

2012 ◽  
Vol 60 (7) ◽  
pp. 1605-1612 ◽  
Author(s):  
Hideyuki Inui ◽  
Tetsuya Takeuchi ◽  
Akari Uesugi ◽  
Fumito Doi ◽  
Mikio Takai ◽  
...  
Keyword(s):  
Polychlorinated Biphenyls ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Single Chain Variable Fragment ◽  
Single Chain ◽  
Coplanar Polychlorinated Biphenyls

Production and characterization of a single-chain variable fragment-alkaline phosphatase fusion protein for glycocholic acid detection in a one-step enzyme-linked immunosorbent assay

2018 ◽  
Vol 10 (22) ◽  
pp. 2629-2635 ◽  
Author(s):  
Xiping Cui ◽  
Qiyi He ◽  
Ding Shen ◽  
Zhengyun Jiang ◽  
Yingshan Chen ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Fusion Protein ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Single Chain Variable Fragment ◽  
Single Chain ◽  
Glycocholic Acid ◽  
One Step

One-step enzyme-linked immunosorbent assay for glycocholic acid based on single-chain variable fragment-alkaline phosphatase fusion protein.

scholarly journals Biotinylated single-chain variable fragment-based enzyme-linked immunosorbent assay for glycocholic acid

The Analyst ◽  
2018 ◽  
Vol 143 (9) ◽  
pp. 2057-2065 ◽  
Author(s):  
Xiping Cui ◽  
Natalia Vasylieva ◽  
Ding Shen ◽  
Bogdan Barnych ◽  
Jun Yang ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Competitive Elisa ◽  
Scfv Antibody ◽  
Enzyme Linked Immunosorbent Assay ◽  
Single Chain Variable Fragment ◽  
Single Chain ◽  
Indirect Competitive Elisa ◽  
Glycocholic Acid

An indirect competitive ELISA was developed for GCA detection based on biotinylated scFv antibody.

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