Determination of Total Phenolic Content and Antioxidant Activity of Garlic (Allium sativum) and Elephant Garlic (Allium ampeloprasum) by Attenuated Total Reflectance–Fourier Transformed Infrared Spectroscopy

2011 ◽  
Vol 59 (10) ◽  
pp. 5215-5221 ◽  
Author(s):  
Xiaonan Lu ◽  
Carolyn F. Ross ◽  
Joseph R. Powers ◽  
D. Eric Aston ◽  
Barbara A. Rasco
2012 ◽  
Vol 60 (18) ◽  
pp. 4709-4717 ◽  
Author(s):  
G. Astrid Garzón ◽  
Carlos-Eduardo Narváez-Cuenca ◽  
Rachel E. Kopec ◽  
Andrew M. Barry ◽  
Kenneth M. Riedl ◽  
...  

2021 ◽  
Vol 13 (2) ◽  
pp. 137-142
Author(s):  
Kasta Gurning ◽  
◽  
Sifikal Lumbangaol ◽  
Risanti F. R. Situmorang ◽  
Saronom Silaban ◽  
...  

The research objectives were to identify the secondary metabolite components, total phenolic content and determine the antioxidant activity of the ethanol extract of red betel leaf (Piper crocatum Ruiz & Pav.). The extraction process was carried out by materation using ethanol as a solvent. Determination of total phenolic content was carried out colorimetrically with Folin-Ciocalteu reagent measured at a maximum wavelength of 765 nm. Determination of antioxidant activity using the DPPH method measured by spectrophotometry at a maximum wavelength of 517 nm. The results of phytochemical screening of the ethanolic extract of red betel leaf contain secondary metabolites, including flavonoid, phenolic, tannin, alkaloids, steroids, and triterpenoids. The total phenolic content of the red betel leaf ethanol extract was 0.949±0.003 mg GAE/g d.w. and has antioxidant activity (IC50) 84,656 including strong category as an antioxidant. Keywords: Piper crocatum Ruiz & Pav., Antioxidant, Ethanol extract, Folin-Ciocalteu and DPPH


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