Allergenicity Assessment of the Papaya Ringspot Virus Coat Protein Expressed in Transgenic Rainbow Papaya

2011 ◽  
Vol 59 (18) ◽  
pp. 10006-10012 ◽  
Author(s):  
Gustavo Fermín ◽  
Ronald C. Keith ◽  
Jon Y. Suzuki ◽  
Stephen A. Ferreira ◽  
Douglas A. Gaskill ◽  
...  
Keyword(s):  
Coat Protein ◽  
Ringspot Virus ◽  
Papaya Ringspot Virus ◽  
Virus Coat Protein ◽  
Virus Coat

scholarly journals Papaya Ringspot Virus Coat Protein Gene for Antigen Presentation in Escherichia coli

BMB Reports ◽  
2006 ◽  
Vol 39 (1) ◽  
pp. 16-21 ◽  
Author(s):  
Supawat Chatchen ◽  
Mila Juricek ◽  
Paloma Rueda ◽  
Sunee Kertbundit
Keyword(s):  
Escherichia Coli ◽  
Coat Protein ◽  
Antigen Presentation ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Ringspot Virus ◽  
Papaya Ringspot Virus ◽  
Virus Coat Protein ◽  
Virus Coat

scholarly journals Study of interaction between Papaya ringspot virus coat protein and infected Carica papaya proteins

2021 ◽  
Vol 16 (1) ◽  
pp. 474-480
Author(s):  
Wanwisa Siriwan ◽  
Sittiruk Roytrakul ◽  
Srimek Chowpongpang ◽  
Aroonothai Sawwa
Keyword(s):  
Coat Protein ◽  
Carica Papaya ◽  
Ringspot Virus ◽  
Papaya Ringspot Virus ◽  
Virus Coat Protein ◽  
Virus Coat

The chemical subunit of tobacco ringspot virus coat protein

Virology ◽  
1979 ◽  
Vol 93 (2) ◽  
pp. 398-412 ◽  
Author(s):  
P.W.G. Chu ◽  
R.I.B. Francki
Keyword(s):  
Coat Protein ◽  
Ringspot Virus ◽  
Tobacco Ringspot Virus ◽  
Virus Coat Protein ◽  
Virus Coat

scholarly journals Hibiscus Chlorotic Ringspot Virus Coat Protein Is Essential for Cell-to-Cell and Long-Distance Movement but Not for Viral RNA Replication

PLoS ONE ◽  
2014 ◽  
Vol 9 (11) ◽  
pp. e113347 ◽  
Author(s):  
Shengniao Niu ◽  
Francisco M. Gil-Salas ◽  
Sunil Kumar Tewary ◽  
Ashwin Kuppusamy Samales ◽  
John Johnson ◽  
...  
Keyword(s):  
Coat Protein ◽  
Rna Replication ◽  
Ringspot Virus ◽  
Viral Rna ◽  
Long Distance ◽  
Virus Coat Protein ◽  
Virus Coat ◽  
Long Distance Movement

scholarly journals Transformation of Plum with the Papaya Ringspot Virus Coat Protein Gene and Reaction of Transgenic Plants to Plum Pox Virus

1995 ◽  
Vol 120 (6) ◽  
pp. 943-952 ◽  
Author(s):  
Ralph Scorza ◽  
Laurene Levy ◽  
Vern Damsteegt ◽  
Luz Marcel Yepes ◽  
John Cordts ◽  
...  
Keyword(s):  
Coat Protein ◽  
Kanamycin Resistance ◽  
Ringspot Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Plum Pox Virus ◽  
Papaya Ringspot Virus ◽  
Indolebutyric Acid ◽  
Systemic Spread ◽  
Foreign Genes ◽  
Virus Coat

Transgenic plum plants expressing the papaya ringspot virus (PRV) coat protein (CP) were produced by Agrobacterium-mediated transformation of hypocotyl slices. Hypocotyl slices were cocultivated with Agrobacterium tumefaciens strain C58/Z707 containing the plasmid pGA482GG/CPPRV-4. This plasmid carries the PRVCP gene construct and chimeric NPTII and GUS genes. Shoots were regenerated on Murashige and Skoog salts, vitamins, 2% sucrose, 2.5 μm indolebutyric acid, 7.5 μm thidiazuron, and appropriate antibiotics for selection. Integration of the foreign genes was verified through kanamycin resistance, GUS assays, polymerase chain reaction (PCR), and Southern blot analyses. Four transgenic clones were identified. Three were vegetatively propagated and graft-inoculated with plum pox virus (PPV)-infected budwood in a quarantine, containment greenhouse. PPV infection was evaluated over a 2- to 4-year period through visual symptoms, enzyme-linked immunosorbent assay, and reverse transcriptase PCR assays. While most plants showed signs of infection and systemic spread of PPV within l-6 months, one plant appeared to delay the spread of virus and the appearance of disease symptoms. Virus spread was limited to basal portions of this plant up to 19 months postinoculation, but, after 32 months symptoms were evident and virus was detected throughout the plant. Our results suggest that heterologous protection with PRVCP, while having the potential to delay PPV symptoms and spread throughout plum plants, may not provide an adequate level of long-term resistance.

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