New Analogues of (E)-β-Farnesene with Insecticidal Activity and Binding Affinity to Aphid Odorant-Binding Proteins

2011 ◽  
Vol 59 (6) ◽  
pp. 2456-2461 ◽  
Author(s):  
Yufeng Sun ◽  
Huili Qiao ◽  
Yun Ling ◽  
Shaoxiang Yang ◽  
Changhui Rui ◽  
...  
Keyword(s):  
Binding Affinity ◽  
Insecticidal Activity ◽  
Binding Proteins ◽  
Odorant Binding Proteins ◽  
Odorant Binding

scholarly journals Characterization of the Expression and Functions of Two Odorant-Binding Proteins of Sitophilus zeamais Motschulsky (Coleoptera: Curculionoidea)

Insects ◽  
2019 ◽  
Vol 10 (11) ◽  
pp. 409 ◽  
Author(s):  
Zhang ◽  
Shen ◽  
Xia ◽  
Wang ◽  
Tang
Keyword(s):  
Binding Affinity ◽  
Binding Proteins ◽  
Competitive Binding ◽  
Sitophilus Zeamais ◽  
Host Volatiles ◽  
Binding Assays ◽  
Odorant Binding Proteins ◽  
Qrt Pcr ◽  
Odorant Binding ◽  
Competitive Binding Assays

Odorant-binding proteins (OBPs) are important in insect chemical communication. The objective of this research was to identify the functions of two OBPs in Sitophilus zeamais. qRT-PCR and western blot (WB) were performed to investigate the expression profiles at the transcript and protein levels, respectively. Fluorescence competitive binding assays were used to measure the ability of the OBPs to bind to host volatiles, and a Y-tube olfactometer was used to verify the results (attraction/no response) via behavioral experiments. The RNAi was used to verify the function by knocking down the ability of proteins to bind odorants. qRT-PCR showed the highest expression SzeaOBP1 and SzeaOBP28 at the low-instar larva (LL) and eclosion adult (EA) stages, respectively. WB showed that both SzeaOBP1 and SzeaOBP28 were highly expressed in the EA stage. Fluorescence competitive binding assays indicated that SzeaOBP1 exhibited extremely high binding affinity with cetanol. SzeaOBP28 exhibited a pronounced binding affinity for 4-hydroxy-3-methoxybenzaldehyde. The behavioral experiment showed that the adult S. zeamais responded strongly to 4-hydroxy-3-methoxybenzaldehyde and valeraldehyde from Sorghum bicolor. The RNAi knockdown individuals displayed behavioral differences between normal insects and dsRNA (SzeaOBP1)-treated insects. We infer that they both have functions in perception and recognition of host volatiles, whereas SzeaOBP28 may also have other functions.

scholarly journals GOBP1 Plays a Key Role in Sex Pheromones and Plant Volatiles Recognition in Yellow Peach Moth, Conogethes punctiferalis (Lepidoptera: Crambidae)

Insects ◽  
2019 ◽  
Vol 10 (9) ◽  
pp. 302
Author(s):  
Jing ◽  
Zhang ◽  
Bai ◽  
Prabu ◽  
He ◽  
...  
Keyword(s):  
Host Plant ◽  
Binding Affinity ◽  
Plant Volatiles ◽  
Sex Pheromones ◽  
Binding Proteins ◽  
Prunus Persica ◽  
Olfactory Sensilla ◽  
Odorant Binding Proteins ◽  
Yellow Peach Moth ◽  
Odorant Binding

Insects recognize odorous compounds using sensory neurons organized in olfactory sensilla. The process odor detection in insects requires an ensemble of proteins, including odorant binding proteins, olfactory receptors, and odor degrading enzymes; each of them are encoded by multigene families. Most functional proteins seem to be broadly tuned, responding to multiple chemical compounds with different, but mostly quite similar structures. Based on the hypothesis that insects recognize host volatiles by means of general odorant binding proteins (GOBPs), the current study aimed to characterize GOBPs of the yellow peach moth, Conogethes punctiferalis (Guenée). In oviposition preference tests, it was found that the yellow peach moth preferred volatiles from Prunus persica (peach) in finding their host plant. Exposure of the moth to volatiles from peaches affected the expression level of GOBP genes. Binding affinity of GOBPs from yellow peach moth was assessed for 16 host plant volatiles and 2 sex pheromones. The fluorescence ligand-binding assays revealed highest affinities for hexadecanal, farnesol, and limonene with KD values of 0.55 ± 0.08, 0.35 ± 0.04, and 1.54 ± 0.39, respectively. The binding sites of GOBPs from yellow peach moth were predicted using homology modeling and characterized using molecular docking approaches. The results indicated the best binding affinity of both GOBP1 and GOBP2 for farnesol, with scores of −7.4 and −8.5 kcal/mol. Thus, GOBPs may play an important role in the process of finding host plants.

scholarly journals Identification and functional characterization of odorant-binding proteins 69a and 76a of Drosophila suzukii

Heliyon ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. e06427
Author(s):  
Haixia Zhan ◽  
Du Li ◽  
Youssef Dewer ◽  
Changying Niu ◽  
Fengqi Li ◽  
...  
Keyword(s):  
Binding Proteins ◽  
Functional Characterization ◽  
Drosophila Suzukii ◽  
Odorant Binding Proteins ◽  
Odorant Binding

Identification and expression profiles analysis of odorant‐binding proteins in soybean aphid, Aphis glycines (Hemiptera: Aphididae)

2019 ◽  
Vol 27 (5) ◽  
pp. 1019-1030 ◽  
Author(s):  
Ling Wang ◽  
Ying‐Dong Bi ◽  
Ming Liu ◽  
Wei Li ◽  
Miao Liu ◽  
...  
Keyword(s):  
Binding Proteins ◽  
Expression Profiles ◽  
Soybean Aphid ◽  
Aphis Glycines ◽  
Odorant Binding Proteins ◽  
Odorant Binding

scholarly journals Pheromone Binding to General Odorant-binding Proteins from the Navel Orangeworm

2010 ◽  
Vol 36 (7) ◽  
pp. 787-794 ◽  
Author(s):  
Zhao Liu ◽  
Diogo M. Vidal ◽  
Zainulabeuddin Syed ◽  
Yuko Ishida ◽  
Walter S. Leal
Keyword(s):  
Binding Proteins ◽  
Navel Orangeworm ◽  
Odorant Binding Proteins ◽  
Odorant Binding
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