Theaflavins Depolymerize Microtubule Network through Tubulin Binding and Cause Apoptosis of Cervical Carcinoma HeLa Cells

2011 ◽  
Vol 59 (5) ◽  
pp. 2040-2048 ◽  
Author(s):  
Subhendu Chakrabarty ◽  
Amlan Das ◽  
Abhijit Bhattacharya ◽  
Gopal Chakrabarti
Keyword(s):  
Cervical Carcinoma ◽  
Hela Cells ◽  
Microtubule Network ◽  
Tubulin Binding

Bid and Calpains Cooperate to Trigger Oxaliplatin-Induced Apoptosis of Cervical Carcinoma HeLa Cells

2009 ◽  
Vol 76 (5) ◽  
pp. 998-1010 ◽  
Author(s):  
Sergio Anguissola ◽  
Barbara Köhler ◽  
Robert O'Byrne ◽  
Heiko Düssmann ◽  
Mary D. Cannon ◽  
...  
Keyword(s):  
Cervical Carcinoma ◽  
Hela Cells ◽  
Induced Apoptosis

scholarly journals 08-P013 Mercury (Drosophila Tubulin Binding Cofactor B) controls cell polarity through the stabilisation of the microtubule network

2009 ◽  
Vol 126 ◽  
pp. S147-S148
Author(s):  
Alexandre Baffet ◽  
Beatrice Benoit ◽  
Vanessa Gourhand ◽  
Claire Heichette ◽  
Denis Chretien ◽  
...  
Keyword(s):  
Cell Polarity ◽  
Microtubule Network ◽  
Tubulin Binding

scholarly journals Girdin expression in cervical carcinoma and its role in the malignant properties of HeLa cells

2016 ◽  
Vol 11 (4) ◽  
pp. 2440-2444 ◽  
Author(s):  
PING JIANG ◽  
YA-LI REN ◽  
JIA-LIANG LI ◽  
JUN LUO
Keyword(s):  
Cervical Carcinoma ◽  
Hela Cells

Induction of apoptosis in cervical carcinoma HeLa cells by Petasites japonicus ethanol extracts

2015 ◽  
Vol 24 (2) ◽  
pp. 665-672 ◽  
Author(s):  
Yu-Jin Hwang ◽  
Hae-Ri Wi ◽  
Haeng-Ran Kim ◽  
Kye Won Park ◽  
Kyung-A Hwang
Keyword(s):  
Cervical Carcinoma ◽  
Hela Cells ◽  
Induction Of Apoptosis ◽  
Ethanol Extracts ◽  
Petasites Japonicus

scholarly journals Effect of miR-301a/PTEN pathway on the proliferation and apoptosis of cervical cancer

2019 ◽  
Vol 25 (4) ◽  
pp. 217-223 ◽  
Author(s):  
Li-na Peng ◽  
Wen-tian Shi ◽  
Huan-rong Feng ◽  
Chuan-yu Wei ◽  
Qi-nan Yin
Keyword(s):  
Cervical Cancer ◽  
Cervical Carcinoma ◽  
Hela Cells ◽  
Pten Expression ◽  
Cell Counting ◽  
Luciferase Assay ◽  
Loss Of Function ◽  
Reverse Pattern ◽  
Significant Difference ◽  
Direct Target

The aim of this study was to evaluate the effect of the miR-301a/PTEN pathway in cervical cancer. miR-301a and PTEN expression were measured by quantitative real-time PCR (qRT-PCR) in tissues samples and HeLa cells. PTEN protein level was determined by Western blotting. Dual reporter luciferase assay was performed to validate PTEN as a direct target of miR-301a. The gain- and loss-of function assay was performed by miR-301a overexpression and silencing. Cell proliferation was monitored by cell counting Kit-8 (CCK-8). Cell apoptosis was quantitated by flow cytometry. SPSS was used to analyze the significant difference in the treatments. miR-301a demonstrated a significantly higher expression in cervical carcinoma tissues compared with the paired non-carcinoma tissues ( n = 12), while PTEN expression was found to be significantly lower in cervical carcinoma tissues than their paired non-carcinoma tissues ( n = 12). In addition, PTEN was identified as the direct target of miR-301a. Moreover, overexpression of miR-301a significantly promoted HeLa cells proliferation and anti-apoptosis which had a reverse pattern after PTEN overexpression. Our results confirm PTEN as a direct target of miR-301a in HeLa cells and suggest that miR-301a/PTEN pathway contributes to the development and progression of cervical cancer.

scholarly journals Tubulin Folding Cofactor TBCB is a Target of the Salmonella Effector Protein SseK1

2020 ◽  
Vol 21 (9) ◽  
pp. 3193 ◽  
Author(s):  
Juan Luis Araujo-Garrido ◽  
Fernando Baisón-Olmo ◽  
Joaquín Bernal-Bayard ◽  
Francisco Romero ◽  
Francisco Ramos-Morales
Keyword(s):  
Host Cell ◽  
Type Iii Secretion ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Yeast Two Hybrid ◽  
Microtubule Network ◽  
Binding Partner ◽  
Tubulin Binding ◽  
Serovar Typhimurium ◽  
Novel Target ◽  
Two Hybrid

Salmonella enterica serovar Typhimurium is a human and animal pathogen that uses type III secretion system effectors to manipulate the host cell and fulfill infection. SseK1 is a Salmonella effector with glycosyltransferase activity. We carried out a yeast two-hybrid screen and have identified tubulin-binding cofactor B (TBCB) as a new binding partner for this effector. SseK1 catalyzed the addition of N-acetylglucosamine to arginine on TBCB, and its expression promoted the stabilization of the microtubule cytoskeleton of HEK293T cells. The conserved Asp-x-Asp (DxD) motif that is essential for the activity of SseK1 was required for the binding and modification of TBCB and for the effect on the cytoskeleton. Our study has identified a novel target for SseK1 and suggests that this effector may have a role in the manipulation of the host cell microtubule network to provide a safe niche for this pathogen.

scholarly journals Cytotoxic activity of proteins isolated from extracts of Corydalis cava tubers in human cervical carcinoma HeLa cells

2010 ◽  
Vol 10 (1) ◽  
Author(s):  
Robert Nawrot ◽  
Maria Wolun-Cholewa ◽  
Wojciech Bialas ◽  
Danuta Wyrzykowska ◽  
Stanislaw Balcerkiewicz ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Cervical Carcinoma ◽  
Hela Cells ◽  
Human Cervical Carcinoma
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