Characterization of Allergens Isolated from the Freshwater Fish Blunt Snout Bream (Megalobrama amblycephala)

2011 ◽  
Vol 59 (1) ◽  
pp. 458-463 ◽  
Author(s):  
Rong Liu ◽  
Hari B. Krishnan ◽  
Wentong Xue ◽  
Chuyi Liu
Keyword(s):  
Freshwater Fish ◽  
Megalobrama Amblycephala ◽  
Blunt Snout Bream

scholarly journals Molecular Characterization of PGC-1β (PPAR Gamma Coactivator 1β) and its Roles in Mitochondrial Biogenesis in Blunt Snout Bream (Megalobrama amblycephala)

2020 ◽  
Vol 21 (6) ◽  
pp. 1935 ◽  
Author(s):  
Kangle Lu ◽  
Tomas Policar ◽  
Xiaojun Song ◽  
Samad Rahimnejad
Keyword(s):  
Molecular Characterization ◽  
Mitochondrial Biogenesis ◽  
Phylogenetic Analyses ◽  
Ppar Gamma ◽  
Megalobrama Amblycephala ◽  
Fat Intake ◽  
High Fat ◽  
Blunt Snout Bream ◽  
Mitochondria Biogenesis

This study aimed at achieving the molecular characterization of peroxisome proliferator-activated receptor-gamma coactivator 1β (PGC-1β) and exploring its modulatory roles in mitochondria biogenesis in blunt snout bream (Megalobrama amblycephala). A full-length cDNA of PGC-1β was cloned from liver which covered 3110 bp encoding 859 amino acids. The conserved motifs of PGC-1β family proteins were gained by MEME software, and the phylogenetic analyses showed motif loss and rearrangement of PGC-1β in fish. The function of PGC-1β was evaluated through overexpression and knockdown of PGC-1β in primary hepatocytes of blunt snout bream. We observed overexpression of PGC-1β along with enhanced mitochondrial transcription factor A (TFAM) expression and mtDNA copies in hepatocytes, and its knockdown led to slightly reduced NRF1 expression. However, knockdown of PGC-1β did not significantly influence TFAM expression or mtDNA copies. The alterations in mitochondria biogenesis were assessed following high-fat intake, and the results showed that it induces downregulation of PGC-1β. Furthermore, significant decreases in mitochondrial respiratory chain activities and mitochondria biogenesis were observed by high-fat intake. Our findings demonstrated that overexpression of PGC-1β induces the enhancement of TFAM expression and mtDNA amount but not NRF-1. Therefore, it could be concluded that PGC-1β is involved in mitochondrial biogenesis in blunt snout bream but not through PGC-1β/NRF-1 pathway.

Establishment and characterization of a fin cell line from blunt snout bream, Megalobrama amblycephala

2013 ◽  
Vol 39 (6) ◽  
pp. 1399-1410 ◽  
Author(s):  
Dong-Mei Zhu ◽  
Kun Yang ◽  
Wei-Min Wang ◽  
Wen Song
Keyword(s):  
Cell Line ◽  
Megalobrama Amblycephala ◽  
Blunt Snout Bream

Characterization of GHRs, IGFs and MSTNs, and analysis of their expression relationships in blunt snout bream, Megalobrama amblycephala

Gene ◽  
2014 ◽  
Vol 535 (2) ◽  
pp. 239-249 ◽  
Author(s):  
Cong Zeng ◽  
Xiao-lian Liu ◽  
Wei-min Wang ◽  
Jin-gou Tong ◽  
Wei Luo ◽  
...  
Keyword(s):  
Megalobrama Amblycephala ◽  
Blunt Snout Bream

Discovery and characterization of conserved and novel microRNAs from blunt snout bream ( Megalobrama amblycephala ) by deep sequencing

Gene ◽  
2018 ◽  
Vol 654 ◽  
pp. 57-63
Author(s):  
Yong Huang ◽  
Wangbao Gong ◽  
Jianli Xiong ◽  
Xiao Chan Gao ◽  
Hong Tao Ren
Keyword(s):  
Deep Sequencing ◽  
Megalobrama Amblycephala ◽  
Blunt Snout Bream
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