Efficient Screening of a Novel Antimicrobial Peptide from Jatropha curcas by Cell Membrane Affinity Chromatography

2011 ◽  
Vol 59 (4) ◽  
pp. 1145-1151 ◽  
Author(s):  
Jianhui Xiao ◽  
Hui Zhang ◽  
Liya Niu ◽  
Xingguo Wang
Keyword(s):  
Cell Membrane ◽  
Antimicrobial Peptide ◽  
Affinity Chromatography ◽  
Jatropha Curcas ◽  
Membrane Affinity ◽  
Efficient Screening

An antimicrobial peptide screened from casein hydrolyzate by Saccharomyces cerevisiae cell membrane affinity method

Food Control ◽  
2015 ◽  
Vol 50 ◽  
pp. 413-422 ◽  
Author(s):  
Wenting Tang ◽  
Huaning Yuan ◽  
Hui Zhang ◽  
Li Wang ◽  
Haifeng Qian ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Membrane ◽  
Antimicrobial Peptide ◽  
Membrane Affinity ◽  
Casein Hydrolyzate

scholarly journals Quantitative Analysis of the Membrane Affinity of Local Anesthetics Using a Model Cell Membrane

Membranes ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 579
Author(s):  
Wanjae Choi ◽  
Hyunil Ryu ◽  
Ahmed Fuwad ◽  
Seulmini Goh ◽  
Chaoge Zhou ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Local Anesthetics ◽  
Electrophysiological Study ◽  
Local Area ◽  
Membrane Properties ◽  
Dominant Factor ◽  
Free Standing ◽  
Membrane Affinity ◽  
Conformational Effects ◽  
Model Protein

Local anesthesia is a drug that penetrates the nerve cell membrane and binds to the voltage gate sodium channel, inhibiting the membrane potential and neurotransmission. It is mainly used in clinical uses to address the pain of surgical procedures in the local area. Local anesthetics (LAs), however, can be incorporated into the membrane, reducing the thermal stability of the membrane as well as altering membrane properties such as fluidity, permeability, and lipid packing order. The effects of LAs on the membrane are not yet fully understood, despite a number of previous studies. In particular, it is necessary to analyze which is the more dominant factor, the membrane affinity or the structural perturbation of the membrane. To analyze the effects of LAs on the cell membrane and compare the results with those from model membranes, morphological analysis and 50% inhibitory concentration (IC50) measurement of CCD-1064sk (fibroblast, human skin) membranes were carried out for lidocaine (LDC) and tetracaine (TTC), the most popular LAs in clinical use. Furthermore, the membrane affinity of the LAs was quantitatively analyzed using a colorimetric polydiacetylene assay, where the color shift represents their distribution in the membrane. Further, to confirm the membrane affinity and structural effects of the membranes, we performed an electrophysiological study using a model protein (gramicidin A, gA) and measured the channel lifetime of the model protein on the free-standing lipid bilayer according to the concentration of each LA. Our results show that when LAs interact with cell membranes, membrane affinity is a more dominant factor than steric or conformational effects of the membrane.

Reduced cell membrane affinity for lithium ion during maintenance treatment of bipolar affective disorder

1990 ◽  
Vol 27 (7) ◽  
pp. 795-798 ◽  
Author(s):  
Alan G. Mallinger ◽  
Jonathan M. Himmelhoch ◽  
Michael E. Thase ◽  
Christine Dippold ◽  
Steven Knopf
Keyword(s):  
Cell Membrane ◽  
Affective Disorder ◽  
Maintenance Treatment ◽  
Bipolar Affective Disorder ◽  
Lithium Ion ◽  
Membrane Affinity

scholarly journals An Escherichia coli Cell Membrane Chromatography-Offline LC-TOF-MS Method for Screening and Identifying Antimicrobial Peptides from Jatropha curcas Meal Protein Isolate Hydrolysates

2012 ◽  
Vol 17 (6) ◽  
pp. 752-760 ◽  
Author(s):  
Jianhui Xiao ◽  
Hui Zhang
Keyword(s):  
Escherichia Coli ◽  
Cell Membrane ◽  
Antimicrobial Peptides ◽  
Jatropha Curcas ◽  
High Throughput Screening ◽  
Protein Isolate ◽  
Shigella Dysenteriae ◽  
Cationic Antimicrobial Peptide ◽  
Meal Protein ◽  
Tof Ms

A novel, simple, and rapid method, named cell membrane affinity extraction (CMAE)–offline liquid chromatography time-of-flight mass spectrometry (LC-TOF-MS) was developed for screening and identifying antimicrobial peptides from Jatropha curcas meal protein isolate hydrolysates (JCMPIH) obtained by proteolytic enzyme (pepsin, trypsin, protamex, neutrase, flavourzyme, papain, alcalase, and acid protease) hydrolysis. A cationic antimicrobial peptide (CAILTHKR, JCpep8) was successfully isolated and identified by this method. Antimicrobial assay indicated that JCpep8 was active against the tested microorganisms ( Escherichia coli ATCC 25922, Shigella dysenteriae ATCC 51302, Pseudomonas aeruginosa ATCC 27553, Staphylococcus aureus ATCC 25923, Bacillus subtilis ATCC 23631, Streptococcus pneumoniae ATCC 49619) with minimal inhibitory concentration values ranging from 29 to 68 µg/mL. JCpep8 induced significant morphological alterations of the tested microbe surfaces, as shown by transmission electron microscopy, indicating strong membrane disruption. The results showed that CMAE-offline LC-TOF-MS could be a promising method for discovering high-throughput screening antimicrobial peptides from JCMPIH.

scholarly journals Activity of Antimicrobial Peptide Aggregates Decreases with Increased Cell Membrane Embedding Free Energy Cost

Biochemistry ◽  
2018 ◽  
Vol 57 (18) ◽  
pp. 2606-2610 ◽  
Author(s):  
Rongfeng Zou ◽  
Xiaomin Zhu ◽  
Yaoquan Tu ◽  
Junchen Wu ◽  
Markita P. Landry
Keyword(s):  
Free Energy ◽  
Cell Membrane ◽  
Antimicrobial Peptide ◽  
Energy Cost

A Pluronic-coupled metal-chelating ligand for membrane affinity chromatography

2006 ◽  
Vol 279 (1-2) ◽  
pp. 120-128 ◽  
Author(s):  
S. Govender ◽  
W.J. Przybylowicz ◽  
E.P. Jacobs ◽  
M.W. Bredenkamp ◽  
L. van Kralingen ◽  
...  
Keyword(s):  
Affinity Chromatography ◽  
Metal Chelating ◽  
Membrane Affinity ◽  
Chelating Ligand
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