Fiber Reduction and Lipid Enrichment in Carotenoid-Enriched Distillers Dried Grain with Solubles Produced by Secondary Fermentation ofPhaffia rhodozymaandSporobolomyces roseus

2010 ◽  
Vol 58 (24) ◽  
pp. 12744-12748 ◽  
Author(s):  
Nanjundaswamy Ananda ◽  
Praveen V. Vadlani
2020 ◽  
Author(s):  
Cassandra L. Pegg ◽  
Toan K. Phung ◽  
Christopher H. Caboche ◽  
Suchada Niamsuphap ◽  
Marshall Bern ◽  
...  

ABSTRACTSparkling wine is an alcoholic beverage enjoyed around the world. The sensory properties of sparkling wine depend on a complex interplay between the chemical and biochemical components in the final product. Glycoproteins have been linked to positive and negative qualities in sparkling wine, but the glycosylation profiles of sparkling wine have not been previously investigated in detail. We analysed the glyco/proteome of sparkling wines using protein- and glycopeptide-centric approaches. We developed an automated workflow that created ion libraries to analyse Sequential Window Acquisition of all THeoretical mass spectra (SWATH) Data Independent Acquisition (DIA) mass spectrometry data based on glycopeptides identified by Byonic. We applied our workflow to three pairs of experimental sparkling wines to assess the effects of aging on lees and of different yeast strains used in the Liqueur de Tirage for secondary fermentation. We found that aging a cuvée on lees for 24 months compared to 8 months led to a dramatic decrease in overall protein abundance and an enrichment in large glycans at specific sites in some proteins. Secondary fermentation of a Riesling wine with Saccharomyces cerevisiae yeast strain Siha4 produced more yeast proteins and glycoproteins than with S. cerevisiae yeast strain DV10. The abundance and glycosylation profiles of grape glycoproteins were also different between grape varieties. This work represents the first in-depth study into protein- and peptide-specific glycosylation in sparkling wines and describes a quantitative glycoproteomic SWATH/DIA workflow that is broadly applicable to other sample types.


2019 ◽  
Vol 35 (21) ◽  
pp. 4507-4508 ◽  
Author(s):  
Geremy Clair ◽  
Sarah Reehl ◽  
Kelly G Stratton ◽  
Matthew E Monroe ◽  
Malak M Tfaily ◽  
...  

Abstract Summary Here we introduce Lipid Mini-On, an open-source tool that performs lipid enrichment analyses and visualizations of lipidomics data. Lipid Mini-On uses a text-mining process to bin individual lipid names into multiple lipid ontology groups based on the classification (e.g. LipidMaps) and other characteristics, such as chain length. Lipid Mini-On provides users with the capability to conduct enrichment analysis of the lipid ontology terms using a Shiny app with options of five statistical approaches. Lipid classes can be added to customize the user’s database and remain updated as new lipid classes are discovered. Visualization of results is available for all classification options (e.g. lipid subclass and individual fatty acid chains). Results are also visualized through an editable network of relationships between the individual lipids and their associated lipid ontology terms. The utility of the tool is demonstrated using biological (e.g. human lung endothelial cells) and environmental (e.g. peat soil) samples. Availability and implementation Rodin (R package: https://github.com/PNNL-Comp-Mass-Spec/Rodin), Lipid Mini-On Shiny app (https://github.com/PNNL-Comp-Mass-Spec/LipidMiniOn) and Lipid Mini-On online tool (https://omicstools.pnnl.gov/shiny/lipid-mini-on/). Supplementary information Supplementary data are available at Bioinformatics online.


Author(s):  
Lourdes Marchante ◽  
Adela Mena ◽  
Pedro M Izquierdo‐Cañas ◽  
Esteban García‐Romero ◽  
María Soledad Pérez‐Coello ◽  
...  

1979 ◽  
Vol 41 (2) ◽  
pp. 393-397 ◽  
Author(s):  
J. B. Rowe ◽  
Marion L. Loughnan ◽  
J. V. Nolan ◽  
R. A. Leng

1995 ◽  
Vol 88 (3) ◽  
pp. 311-318 ◽  
Author(s):  
Richard H. Neary ◽  
Mark D. Kilby ◽  
Padma Kumpatula ◽  
Francis L. Game ◽  
Deepak Bhatnagar ◽  
...  

1. Lipid, apolipoprotein concentration and composition were determined in maternal venous and umbilical arterial and venous blood at delivery by elective Caesarean section in 13 full-term pregnancies and in 25 healthy non-pregnant females. The indications of Caesarean section were a previous Caesarean section or breech presentation. None of the women was in labour and there were no other complications of pregnancy or fetal distress. 2. The objectives of the study were to establish whether the placenta has a role in feto-maternal cholesterol metabolism through either synthesis or transplacental cholesterol flux. The potential for free cholesterol diffusion between mother and fetus and rates of cholesterol esterification and transfer between lipoproteins were determined and related to the differences in composition between fetal and maternal lipoproteins. 3. Pregnant women had raised levels of all lipid and lipoprotein fractions compared with control subjects. The greatest increases were in free cholesterol and triacylglycerol (P < 0.0001). Lipoprotein (a) levels were significantly greater in the pregnant women [112(12.2) mg/l] than in the control women [50 (10.0) mg/l]. 4. The only significant correlation between maternal and fetal lipoprotein concentrations was in lipoprotein (a) levels (r = 0.791, P = 0.002). In both umbilical venous and arterial blood, concentrations of very-low- and low-density lipoproteins, particularly apolipoprotein B, cholesteryl ester and triacylglycerol, were lower than in maternal blood (P < 0.0001), but high-density lipoprotein levels were similar. 5. There was no umbilical arteriovenous differences in lipoprotein concentration or composition. This suggests that cholesterol synthesis or free cholesterol diffusion does not occur in the placenta. The relative concentrations of free cholesterol to phospholipid in maternal and fetal lipoproteins do not indicate the existence of a concentration gradient favouring free cholesterol diffusion across the placenta. 6. The esterification of free cholesterol was significantly reduced in maternal [17.7 (2.4) μmol h−1 l−1, P < 0.001] and fetal [6.7 (3.5) μmol h−1 l−1, P < 0.0001] compared with control [40.9 (13.2) μmol h−1 l−1] blood. 7. In fetal compared with maternal high-density lipoproteins the ratios cholesteryl ester/apoliproprotein A-I [0.84 (0.35) versus 0.40 (0.05), P < 0.01] and phospholipid/apolipoprotein A-I [1.66 (0.14) versus 0.58 (0.10), P < 0.0001] indicated lipid enrichment of these particles in the fetus. 8. Lipid enrichment of high-density lipoprotein is due in part to a marked reduction in transfer of cholesteryl ester in the fetus [1.0 (0.6) μmol h−1 l−1] compared with maternal [6.15 (1.3) μmol h−1 l−1, P = 0.004] and control [17.3 (7.2) μmol h−1 l−1, P < 0.0001] blood. 9. In conclusion, there was no evidence for involvement of the placenta in cholesterol metabolism during pregnancy. In fetal life high-density lipoproteins are lipid rich, partly because of a reduction in transfer of esterified cholesterol to other particles. Maternal and fetal lipoprotein levels are not correlated, although the results suggested that lipoprotein (a) levels may be related.


2014 ◽  
Vol 1010-1012 ◽  
pp. 917-921
Author(s):  
Ju Chi Kuang

The paper expounds the principles and technology of earthworm decomposition of urban living garbage, analyzes the key factors influencing the earthworm activity and decomposition of urban household garbage. The experimental results show that the earthworm processing waste suitable conditions are 23-27 °C for temperature, 60%-80% for humidity, 6-8 for the soil pH. Visible, pH and temperature have much effect on the earthworm activity, when pH< 5.0 or pH > 8.0, or when the temperature is less than 0°C or more than 35°C, the earthworms can't basically survive. The introduction of the earthworm treatment makes the secondary fermentation of compost greatly shorten the processing cycle. At the same time earthworms’ decomposing garbage significantly increases the soil fertilizer effect.


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