Latent and Active Polyphenol Oxidase (PPO) in Red Clover (Trifolium pratense) and Use of a Low PPO Mutant To Study the Role of PPO in Proteolysis Reduction

2008 ◽  
Vol 56 (8) ◽  
pp. 2817-2824 ◽  
Author(s):  
Ana L. Winters ◽  
Frank R. Minchin ◽  
Terry P. T. Michaelson-Yeates ◽  
Michael R. F. Lee ◽  
Phillip Morris
Keyword(s):  
Polyphenol Oxidase ◽  
Trifolium Pratense ◽  
Red Clover

Étude de l'influence des réserves azotées dans l'estimation par 15N de la fixation symbiotique d'une plante pérenne, Trifolium pratense

1985 ◽  
Vol 63 (6) ◽  
pp. 991-994
Author(s):  
Chantal Lescure ◽  
Alain Chalamet
Keyword(s):  
Trifolium Pratense ◽  
Control Plant ◽  
Red Clover ◽  
Good Control ◽  
Dinitrogen Fixation ◽  
Dilution Technique ◽  
Trifolium Pratense L ◽  
Perennial Legumes ◽  
Kinetics Of

A 15N dilution technique is proposed to determine the role of nitrogen reserves in the plant on the estimation of dinitrogen fixation, during regrowth of perennial legumes. It is based on labelling of nitrogen compartments of the plant. Since the kinetics of utilization of nitrogen reserves of ryegrass (Lolium italicum L.) and red clover (Trifolium pratense L.) appear similar, ryegrass would be a good control plant. Despite this observation, the comparison of two methods (based on two or three sources of nitrogen) to estimate dinitrogen fixation shows the difficulty in applying the technique using 15N-labelled substrate over earlier periods of regrowth. In this case, the 15N method described for determining symbiotic dinitrogen fixation could be applied in controlled conditions.

scholarly journals The Role of Amino Acids for the In Vitro Culture of Some Species of Forage Legumes I. Trifolium pratense L

2013 ◽  
Vol 70 (1) ◽  
pp. 87-92
Author(s):  
Gabriela Maria VICAȘ ◽  
Mircea SAVATTI
Keyword(s):  
Amino Acids ◽  
In Vitro Culture ◽  
Culture Medium ◽  
Trifolium Pratense ◽  
Basal Medium ◽  
Red Clover ◽  
Forage Legumes ◽  
Trifolium Pratense L

Establishing the effect of the amino acids as additional additives to the culture medium is and will be in the future one of our concerns of interest for the in vitro culture of some plants. The present study examines the effect of the glicocol added to the LS basal medium over the embryos of the Trifolium pratense L specie cultivated in vitro. There were followed: the percentage of plant regeneration of the red clover, its multiplication capacity and the formation of the root system, and also the evolution of the callus obtained on mediums with 2,4D, BA and amino acid.

Gene Expression Patterns, Localization, and Substrates of Polyphenol Oxidase in Red Clover (Trifolium pratense L.)

2013 ◽  
Vol 61 (31) ◽  
pp. 7421-7430 ◽  
Author(s):  
K. Judith Webb ◽  
Alan Cookson ◽  
Gordon Allison ◽  
Michael L. Sullivan ◽  
Ana L. Winters
Keyword(s):  
Gene Expression ◽  
Polyphenol Oxidase ◽  
Trifolium Pratense ◽  
Expression Patterns ◽  
Red Clover ◽  
Gene Expression Patterns ◽  
Trifolium Pratense L

scholarly journals Polyphenol oxidase affects normal nodule development in red clover (Trifolium pratense L.)

2014 ◽  
Vol 5 ◽  
Author(s):  
K. Judith Webb ◽  
Alan Cookson ◽  
Gordon Allison ◽  
Michael L. Sullivan ◽  
Ana L. Winters
Keyword(s):  
Polyphenol Oxidase ◽  
Trifolium Pratense ◽  
Red Clover ◽  
Nodule Development ◽  
Trifolium Pratense L

Red clover polyphenol oxidase reduces ruminal lipolysis in in vitro batch culture

2007 ◽  
Vol 2007 ◽  
pp. 25-25
Author(s):  
M. R. F. Lee ◽  
F. R. Minchin ◽  
R. D. Hatfield ◽  
M. L. Sullivan
Keyword(s):  
Batch Culture ◽  
Polyphenol Oxidase ◽  
Saturated Fatty Acids ◽  
Red Clover ◽  
Breeding Strategies ◽  
Animal Products ◽  
Plant Lipids ◽  
Different Levels

It has been shown that the rate of lipolysis and proteolysis differs significantly between red clover genotypes with different levels of polyphenol oxidase (PPO) activity (Lee et al. 2004). Sullivan and Hatfield, (2006) reported the development of genetically modified isolines of red clover with the PPO1 gene silenced. This material was used to examine the role of the red clover PPO enzyme on lipolysis and ultimately C18 polyunsaturated fatty acid biohydrogenation in batch culture. If the role of PPO in reducing ruminal lipolysis of plant lipids is proven it would influence breeding strategies for forages which exhibit this trait in an attempt to increase the levels of beneficial PUFA and decrease detrimental trans and saturated fatty acids in animal products.

The role of medicarpin and maackiain in the response of red clover leaves to Helminthosporium carbonum, Stemphylium botryosum, and S. sarcinaeforme

1976 ◽  
Vol 54 (23) ◽  
pp. 2609-2619 ◽  
Author(s):  
Lorne J. Duczek ◽  
Verna J. Higgins
Keyword(s):  
Trifolium Pratense ◽  
Leaf Tissue ◽  
Red Clover ◽  
Stemphylium Botryosum ◽  
Inhibitory Compounds ◽  
Trifolium Pratense L ◽  
The Difference

Helminthosporium carbonum, a corn pathogen, and Stemphylium botryosum, an alfalfa pathogen, are both nonpathogenic on red clover (Trifolium pratense L.), while S. sarcinaeforme is a foliar pathogen on red clover. In clover leaves challenged with H. carbonum, medicarpin and maackiain were the only inhibitory compounds found in diffusates or in leaf tissue in a concentration sufficient to account for the inhibition of this fungus. Helminthosporium carbonum was inhibited by and could not degrade medicaipin and (or) maackiain in vitro. Both S. botryosum and S. sarcinaeforme were only slightly inhibited by these compounds in mycelial growth bioassays, and both fungi degraded medicarpin and (or) maackiain in vitro and some evidence was obtained that degradation occurred in vivo. In contrast with the relatively high amounts of medicarpin and maackiain that accumulated in leaves challenged with H. carbonum, relatively low amounts accumulated in leaves challenged with either S. botryosum or S. sarcinaeforme. The evidence suggests that the resistance of clover to H. carbonum can be accounted for by these phytoalexins; however, differences in relation to accumulation of, inhibition by, and breakdown of medicarpin and (or) maackiain were not enough to explain the difference in pathogenicity of S. botryosum and S. sarcinaeforme on red clover.

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