Event-Specific Qualitative and Quantitative PCR Detection Methods for Transgenic Rapeseed Hybrids MS1×RF1 and MS1×RF2

2007 ◽  
Vol 55 (21) ◽  
pp. 8380-8389 ◽  
Author(s):  
Yuhua Wu ◽  
Gang Wu ◽  
Ling Xiao ◽  
Changming Lu
Keyword(s):  
Quantitative Pcr ◽  
Pcr Detection ◽  
Detection Methods ◽  
Qualitative And Quantitative ◽  
Qualitative And Quantitative Pcr ◽  
Transgenic Rapeseed

Event-specific qualitative and quantitative PCR detection of LY038 maize in mixed samples

Food Control ◽  
2011 ◽  
Vol 22 (8) ◽  
pp. 1287-1295 ◽  
Author(s):  
Nan Zhang ◽  
Wentao Xu ◽  
Weibin Bai ◽  
Zhifang Zhai ◽  
Yunbo Luo ◽  
...  
Keyword(s):  
Quantitative Pcr ◽  
Pcr Detection ◽  
Qualitative And Quantitative ◽  
Qualitative And Quantitative Pcr ◽  
Mixed Samples

scholarly journals Simplex and Duplex Polymerase Chain Reaction Analysis of Herculex® RW (59122) Maize Based on One Reference Molecule Including Separated Fragments of 5 Integration Site and Endogenous Gene

2009 ◽  
Vol 92 (5) ◽  
pp. 1472-1483 ◽  
Author(s):  
Xiang Li ◽  
Litao Yang ◽  
Jianzhong Zhang ◽  
Shu Wang ◽  
Kailin Shen ◽  
...  
Keyword(s):  
Quantitative Pcr ◽  
Genomic Dna ◽  
Raw Materials ◽  
Integration Site ◽  
Polymerase Chain Reaction Analysis ◽  
Detection Methods ◽  
Endogenous Gene ◽  
Qualitative And Quantitative ◽  
Reference Molecule ◽  
Qualitative And Quantitative Pcr

Abstract Reference molecules, as positive controls and calibrators, have been recently developed in genetically modified organism analysis as a potential substitute for reference materials derived from plant raw materials. In this study, a novel reference molecule p59122, including the revealed 5 integration sequence of maize Herculex® RW (59122), was constructed that was suitable for simplex and duplex event-specific qualitative and quantitative PCR detections. The LOD values were 10 copies both for simplex and duplex qualitative PCR when p59122 was used as the calibrator. These values were comparable to those of using genomic DNA samples with 0.01 and 0.05, approximately 5 and 25 hyploid genomic DNA copies, respectively. The absolute LOD and LOQ values were confirmed to be as low as 10 and 25 copies of p59122 DNA both in simplex and duplex quantitative systems. Furthermore, ideal quantification data with low bias, SD and RSD values were obtained from the practical samples analyses in simplex and duplex real-time PCR systems using the reference molecule p59122 as a calibrator. All these results suggested that the developed reference molecule p59122 and the qualitative and quantitative PCR detection methods are suitable for identification and quantification of GM maize 59122 and its derived products.

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