Determination of Lipoxygenase Activity in Plant Extracts Using a Modified Ferrous Oxidation−Xylenol Orange Assay

2007 ◽  
Vol 55 (15) ◽  
pp. 5956-5959 ◽  
Author(s):  
Maria del Carmen Pinto ◽  
Adrián Tejeda ◽  
Antonio L. Duque ◽  
Pedro Macías
Keyword(s):  
Plant Extracts ◽  
Xylenol Orange ◽  
Lipoxygenase Activity ◽  
Ferrous Oxidation

scholarly journals Determination of hydroperoxides in aqueous solutions containing surfactants by the ferrous oxidation-xylenol orange method.

2009 ◽  
Vol 56 (3) ◽  
Author(s):  
Piotr Meisner ◽  
Jerzy L Gebicki
Keyword(s):  
Aqueous Solutions ◽  
Anionic Surfactants ◽  
Xylenol Orange ◽  
Cosmetic Formulations ◽  
Ferrous Oxidation

Some surfactants widely used as additives in food, pharmaceuticals, and cosmetic formulations are susceptible to peroxidation resulting in accumulation of hydroperoxides (HP). Our investigation was aimed to study the possible influence of different surfactants on the proportionality and reproducibility of the ferrous oxidation-xylenol orange method developed originally for the determination of hydroperoxides. We also attempted to apply this method to determine hydroperoxides produced radiolytically in surfactant molecules. From our preliminary studies we conclude that the method can be applied for determination of hydroperoxides in systems containing non-ionic or anionic surfactants provided careful calibration is performed for each surfactant.

scholarly journals Determination of Lipoxygenase Activity in Cereals Grains Using a Silver Nanoparticles Assay

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Raybel Muñoz ◽  
Jose A. Rodriguez ◽  
M. Elena Páez-Hernández ◽  
Irais Sánchez-Ortega ◽  
Eva M. Santos
Keyword(s):  
Silver Nanoparticles ◽  
Enzyme Activity ◽  
Limit Of Detection ◽  
Xylenol Orange ◽  
Enzymatic Reaction ◽  
Lipoxygenase Activity ◽  
Cereal Grains ◽  
Optimal Conditions ◽  
Relative Standard

A spectrophotometric method based on the use of silver nanoparticles is presented for the determination of lipoxygenase activity in cereal grains. The method involves the synthesis of prism silver nanoparticles with a maximum absorption at 752 nm, followed by the abatement of the signal as a consequence of the oxidation produced by hydroperoxides generated in the enzymatic reaction. The quantification of the hydroperoxides produced by reaction of lipoxygenase with linoleic acid allows the determination of the enzyme activity in cereal grains. Under optimal conditions, the linear range of the calibration curve ranges from 1.0 to 5.0 μM, with a limit of detection of 0.34 μM. The method was validated comparing the results with those obtained by ferrous-xylenol orange method. A relative standard deviation < 5.0% was obtained in all cases and no significant differences were observed (p<0.05).

A modified ferrous oxidation-xylenol orange assay for lipoxygenase activity in rice grains

2013 ◽  
Vol 141 (3) ◽  
pp. 2405-2411 ◽  
Author(s):  
Tarinee Timabud ◽  
Jirawat Sanitchon ◽  
Paweena Pongdontri
Keyword(s):  
Xylenol Orange ◽  
Lipoxygenase Activity ◽  
Rice Grains ◽  
Ferrous Oxidation

Capillary zone electrophoretic determination of phenolic compounds in chess (Bromus inermisL.) plant extracts

2006 ◽  
Vol 29 (2) ◽  
pp. 308-313 ◽  
Author(s):  
Dagmar Štěrbová ◽  
Jiří Vlček ◽  
Vlastimil Kubáň
Keyword(s):  
Phenolic Compounds ◽  
Plant Extracts ◽  
Capillary Zone
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