Toward Metrological Traceability for DNA Fragment Ratios in GM Quantification. 2. Systematic Study of Parameters Influencing the Quantitative Determination of MON 810 Corn by Real-Time PCR

2007 ◽  
Vol 55 (9) ◽  
pp. 3258-3267 ◽  
Author(s):  
Diana Charels ◽  
Sylvia Broeders ◽  
Philippe Corbisier ◽  
Stefanie Trapmann ◽  
Heinz Schimmel ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Real Time ◽  
Systematic Study ◽  
Real Time Pcr ◽  
Metrological Traceability ◽  
Dna Fragment ◽  
Mon 810

scholarly journals A one-step method for quantitative determination of uracil in DNA by real-time PCR

2010 ◽  
Vol 38 (21) ◽  
pp. e196-e196 ◽  
Author(s):  
András Horváth ◽  
Beáta G. Vértessy
Keyword(s):  
Quantitative Determination ◽  
Real Time ◽  
Real Time Pcr ◽  
Step Method ◽  
One Step

scholarly journals Applicability of a duplex and four singleplex real-time PCR assays for the qualitative and quantitative determination of wild boar and domestic pig meat in processed food products

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Maria Kaltenbrunner ◽  
Walter Mayer ◽  
Kirsten Kerkhoff ◽  
Rita Epp ◽  
Hermann Rüggeberg ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Wild Boar ◽  
Real Time ◽  
Real Time Pcr ◽  
Processed Food ◽  
Domestic Pig ◽  
The Real ◽  
Qualitative And Quantitative ◽  
Pcr Assays

Abstract Appropriate analytical methods are needed for the detection of food authentication. We investigated the applicability of a duplex real-time PCR assay targeting chromosome 1 and two singleplex real-time PCR assays targeting chromosome 9, both published recently, for the qualitative and quantitative determination of wild boar and domestic pig in processed food products. In addition, two singleplex real-time PCR assays targeting chromosome 7 were tested for their suitability to differentiate the two subspecies. Even by targeting the three genome loci, the probability of misclassification was not completely eliminated. Application of the real-time PCR assays to a total of 35 commercial meat products, including 22 goulash products, revealed that domestic pig DNA was frequently present, even in 14 out of 15 products declared to consist of 100% wild boar. Quantitative results obtained with the real-time PCR assays for wild boar (p < 0.001) and those for domestic pig (p < 0.001) were significantly different. However, the results obtained with the real-time PCR assays for wild boar (r = 0.673; p < 0.001) and those for domestic pig (r = 0.505; p = 0.002) were found to be significantly correlated. If the rules given in the paper are followed, the real-time PCR assays are applicable for routine analysis.

The use of real time PCR for quantitative determination of some propionic bacteria residing on human skin

2012 ◽  
Vol 58 (5) ◽  
pp. 608-612
Author(s):  
A.G. Globa ◽  
Y.I. Alekseev ◽  
V.G. Arzumanyan ◽  
V.A. Zaborova ◽  
A.A. Guridov
Keyword(s):  
Quantitative Determination ◽  
Real Time ◽  
Human Skin ◽  
Real Time Pcr ◽  
Bacterial Species ◽  
Test System ◽  
Control Group ◽  
The Real

A test system has been developed for determination of propionic bacterial species residing on human skin. This system developed in the real time PCR format is applicable for quantitative determination and also detection of genomes of the following Propionibacterium species: P. acnes, P. granulosum and P. avidum. This system was used for analysis of wash samples from the skin of 17 pentathlon sportsmen and 16 students. All three species of propionic bacteria were found in all skin wash samples. However, contamination with P. acnes was two times higher in control group than in the group of pentathlon sportsmen.

scholarly journals Quantitative Determination of Lentiviral Vector Particle Numbers by Real-Time PCR

BioTechniques ◽  
2001 ◽  
Vol 31 (3) ◽  
pp. 520-526 ◽  
Author(s):  
M. Scherr ◽  
K. Battmer ◽  
U. Blömer ◽  
A. Ganser ◽  
M. Grez
Keyword(s):  
Quantitative Determination ◽  
Real Time ◽  
Real Time Pcr ◽  
Lentiviral Vector ◽  
Vector Particle

Quantitative determination of mutton adulteration with single-copy nuclear genes by real-time PCR

2020 ◽  
pp. 128622
Author(s):  
Tingting Li ◽  
Jishi Wang ◽  
Zhiying Wang ◽  
Lu Qiao ◽  
Rui Liu ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Real Time ◽  
Real Time Pcr ◽  
Single Copy ◽  
Nuclear Genes
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