Qualitative and Quantitative Evaluation of Protein Extraction Protocols for Apple and Strawberry Fruit Suitable for Two-Dimensional Electrophoresis and Mass Spectrometry Analysis

2007 ◽  
Vol 55 (5) ◽  
pp. 1663-1673 ◽  
Author(s):  
Qifa Zheng ◽  
Jun Song ◽  
Kristen Doncaster ◽  
Elden Rowland ◽  
David M. Byers
Keyword(s):  
Mass Spectrometry ◽  
Quantitative Evaluation ◽  
Protein Extraction ◽  
Mass Spectrometry Analysis ◽  
Two Dimensional ◽  
Strawberry Fruit ◽  
Spectrometry Analysis ◽  
Two Dimensional Electrophoresis ◽  
Qualitative And Quantitative ◽  
Dimensional Electrophoresis

scholarly journals Optimization of an Efficient Protein Extraction Protocol Compatible with Two-Dimensional Electrophoresis and Mass Spectrometry from Recalcitrant Phenolic Rich Roots of Chickpea (Cicer arietinum L.)

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Moniya Chatterjee ◽  
Sumanti Gupta ◽  
Anirban Bhar ◽  
Sampa Das
Keyword(s):  
Mass Spectrometry ◽  
Protein Extraction ◽  
Sodium Dodecyl ◽  
Good Resolution ◽  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Extraction Protocol ◽  
Phenol Extraction ◽  
Spot Number ◽  
Dimensional Electrophoresis

Two-dimensional electrophoresis and mass spectrometry are undoubtedly two essential tools popularly used in proteomic analyses. Utilization of these techniques however largely depends on efficient and optimized sample preparation, regarded as one of the most crucial steps for recovering maximum amount of reliable information. The present study highlights the optimization of an effective and efficient protocol, capable of extraction of root proteins from recalcitrant phenolic rich tissues of chickpea. The widely applicable TCA-acetone and phenol-based methods have been comparatively evaluated, amongst which the latter appeared to be better suited for the sample. The phenol extraction-based method further complemented with sodium dodecyl sulphate (SDS) and pulsatory treatments proved to be the most suitable method represented by greatest spot number, good resolution, and spot intensities. All the randomly selected spots showed successful identification when subjected to further downstream MALDI-TOF and MS/MS analyses. Hence, the information obtained collectively proposes the present protein extraction protocol to be an effective one that could be applicable for recalcitrant leguminous root samples.

scholarly journals Application of two-dimensional electrophoresis and mass spectrometry to screen endometriosis-related proteins

2014 ◽  
Vol 10 (1) ◽  
pp. 95-100
Author(s):  
HANIKEZI TUERXUN ◽  
YANMEI ZHANG ◽  
FEI JI ◽  
AIXINGZI AILI ◽  
XINHUA YANG ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Related Proteins ◽  
Dimensional Electrophoresis

Two-dimensional electrophoresis of human placental mitochondria and protein identification by mass spectrometry: Toward a human mitochondrial proteome

1998 ◽  
Vol 19 (6) ◽  
pp. 1006-1014 ◽  
Author(s):  
Thierry Rabilloud ◽  
Sylvie Kieffer ◽  
Vincent Procaccio ◽  
Mathilde Louwagie ◽  
Paul L. Courchesne ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Protein Identification ◽  
Two Dimensional ◽  
Mitochondrial Proteome ◽  
Two Dimensional Electrophoresis ◽  
Dimensional Electrophoresis

Protein extraction for two-dimensional electrophoresis from olive leaf, a plant tissue containing high levels of interfering compounds

2003 ◽  
Vol 24 (14) ◽  
pp. 2369-2375 ◽  
Author(s):  
Wei Wang ◽  
Monica Scali ◽  
Rita Vignani ◽  
Antonia Spadafora ◽  
Elisabetta Sensi ◽  
...  
Keyword(s):  
Plant Tissue ◽  
Protein Extraction ◽  
Two Dimensional ◽  
Olive Leaf ◽  
Two Dimensional Electrophoresis ◽  
Dimensional Electrophoresis

Protein extraction fromPhoenix dactylifera L. leaves, a recalcitrant material, for two-dimensional electrophoresis

2008 ◽  
Vol 29 (2) ◽  
pp. 448-456 ◽  
Author(s):  
Sonia Gómez-Vidal ◽  
Manuel Tena ◽  
Luis Vicente Lopez-Llorca ◽  
Jesús Salinas
Keyword(s):  
Protein Extraction ◽  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Dimensional Electrophoresis ◽  
Recalcitrant Material
Sign in / Sign up

Export Citation Format

Share Document