Lipid Hydroperoxide Induced Oxidative Stress Damage and Antioxidant Enzyme Response in Caco-2 Human Colon Cells

2006 ◽  
Vol 54 (12) ◽  
pp. 4476-4481 ◽  
Author(s):  
Subhashinee S. K. Wijeratne ◽  
Susan L. Cuppett
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzyme ◽  
Lipid Hydroperoxide ◽  
Human Colon ◽  
Colon Cells ◽  
Stress Damage ◽  
Antioxidant Enzyme Response

O XVII B.2 Determination of endogenous DNA damage, oxidated DNA bases and oxidative stress in primary human colon cells and modulation by plant ingredients

1997 ◽  
Vol 379 (1) ◽  
pp. S171
Author(s):  
Beatrice L. Pool-Zobel ◽  
Salomon L. Abrahamse ◽  
Daniela Oberreuther ◽  
Sylvia Treptow-van Lishaut ◽  
Gerhard Rechkemmer
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Human Colon ◽  
Dna Bases ◽  
Colon Cells ◽  
And Oxidative Stress

scholarly journals Vitamin C − protective role in oxidative stress conditions induced in human normal colon cells by label free Raman spectroscopy and imaging

2021 ◽  
Author(s):  
Karolina Beton ◽  
Beata Brozek-Pluska
Keyword(s):  
Oxidative Stress ◽  
Raman Spectroscopy ◽  
Vitamin C ◽  
Human Colon ◽  
Protective Role ◽  
Spectroscopic Methods ◽  
Label Free ◽  
Normal Cells ◽  
Colon Cells ◽  
Cancerous Cells

Colorectal cancer is the second most frequently diagnosed cancer worldwide. Conventional diagnostics methods of colorectal cancer, can detect it in advanced stage. Spectroscopic methods, including Raman spectroscopy and imaging, are becoming more and more popular in medical applications, and allow fast, precise and unambiguous differentiation of healthy and cancerous samples. the most important advantage of Raman spectroscopy is ability to identify biomarkers that help in differentiation of healthy and cancerous cells based on biochemistry of sample and spectra typical for: lipids, proteins, DNA. The aim of the study was to evaluate the biochemical and structural features of human colon cell lines based on Raman spectroscopy and imaging: normal cells CCD-18 Co, normal cells CCD-18 Co under oxidative stress conditions, normal cells CCD-18 Co at first treated by using tert-Butyl hydroperoxide and then supplemented by vitamin C in high concentration to show the protective role of vitamin C in micromolar concentrations against ROS by spectroscopic methods. Raman data obtained for normal cells injured by ROS were compared with spectra typical for cancerous cells. Statistically assisted analysis has shown that normal, ROS injured and cancerous human colon cells can be distinguished based on their unique vibrational properties. The research carried out proves that label-free Raman spectroscopy may play an important role in clinical diagnostics differentiation of normal and cancerous colon cells and may be a source of intraoperative information supporting histopathological analysis.

scholarly journals Anti-glycation and anti-oxidative effects of a phenolic-enriched maple syrup extract and its protective effects on normal human colon cells

2017 ◽  
Vol 8 (2) ◽  
pp. 757-766 ◽  
Author(s):  
Weixi Liu ◽  
Zhengxi Wei ◽  
Hang Ma ◽  
Ang Cai ◽  
Yongqiang Liu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Human Colon ◽  
Protective Effects ◽  
Colon Cells ◽  
Maple Syrup ◽  
Normal Human ◽  
Oxidative Effects

Phenolic-enriched maple syrup extract (MSX) inhibits the formation of AGEs and protects normal/non-tumorigenic human colon cells from oxidative stress.

scholarly journals Oxidative stress induced by tBHP in human normal colon cells by label free Raman spectroscopy and imaging: The protective role of natural antioxidants in the form of β-carotene

2021 ◽  
Author(s):  
B. Brozek-Pluska ◽  
K. Beton
Keyword(s):  
Oxidative Stress ◽  
Human Colon ◽  
Protective Role ◽  
Control Group ◽  
Label Free ◽  
Normal Colon ◽  
Stress Injury ◽  
Colon Cells ◽  
Β Carotene

AbstractThe present study aimed to investigate the protective effect of β-carotene on the oxidative stress injury of human normal colon cell line CCD-18Co triggered by tert-Butyl hydroperoxide (tBHP). XTT examination was used to determine cell viability after β-carotene supplementation and to determine the optimal concentration of antioxidant in spectroscopic studies. Cell biochemistry for CCD-18Co control group, after tBHP adding and for cells in β-carotene - tBHP model was studied by using label-free Raman microspectroscopy. Results for stress treated CCD-18Co human colon normal cells and human colon cancer cells Caco-2 based on vibration features were also compared. Pretreatment with β-carotene alleviated damages in CCD-18Co human normal colon cells induced by tBHP and showed the preventative effect on cells apoptosis. Treatment with β-carotene altered the level of ROS investigated based on intensities of Raman peaks typical for lipids, proteins and nucleic acids. Presented study confirmed the antioxidant, protective role of β-carotene against ROS by using spectroscopic label-free Raman techniques.

Gastrointestinal digested Sambucus nigra L. fruit extract protects in vitro cultured human colon cells against oxidative stress

2016 ◽  
Vol 197 ◽  
pp. 648-657 ◽  
Author(s):  
Anna Olejnik ◽  
Mariola Olkowicz ◽  
Katarzyna Kowalska ◽  
Joanna Rychlik ◽  
Radosław Dembczyński ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Human Colon ◽  
Sambucus Nigra ◽  
Fruit Extract ◽  
Colon Cells

The secondary Fusarium metabolite aurofusarin induces oxidative stress, cytotoxicity and genotoxicity in human colon cells

2018 ◽  
Vol 284 ◽  
pp. 170-183 ◽  
Author(s):  
Katharina Jarolim ◽  
Konstantin Wolters ◽  
Lydia Woelflingseder ◽  
Gudrun Pahlke ◽  
Julia Beisl ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Human Colon ◽  
Colon Cells

scholarly journals Vitamin C—Protective Role in Oxidative Stress Conditions Induced in Human Normal Colon Cells by Label-Free Raman Spectroscopy and Imaging

2021 ◽  
Vol 22 (13) ◽  
pp. 6928
Author(s):  
Karolina Beton ◽  
Beata Brozek-Pluska
Keyword(s):  
Oxidative Stress ◽  
Colorectal Cancer ◽  
Raman Spectroscopy ◽  
Vitamin C ◽  
Human Colon ◽  
Protective Role ◽  
Label Free ◽  
Normal Cells ◽  
Colon Cells ◽  
Cancerous Cells

Colorectal cancer is the second most frequently diagnosed cancer worldwide. Conventional diagnostics methods of colorectal cancer can detect it at an advanced stage. Spectroscopic methods, including Raman spectroscopy and imaging, are becoming more and more popular in medical applications, and allow fast, precise, and unambiguous differentiation of healthy and cancerous samples. The most important advantage of Raman spectroscopy is the ability to identify biomarkers that help in the differentiation of healthy and cancerous cells based on biochemistry of sample and spectra typical for lipids, proteins, and DNA. The aim of the study was to evaluate the biochemical and structural features of human colon cell lines based on Raman spectroscopy and imaging: normal cells CCD-18 Co, normal cells CCD-18 Co under oxidative stress conditions, and normal cells CCD-18 Co at first treated by using tert-Butyl hydroperoxide and then supplemented by vitamin C in high concentration to show the protective role of vitamin C in micromolar concentrations against ROS (Reactive Oxygen Species). Raman data obtained for normal cells injured by ROS were compared with spectra typical for cancerous cells. Statistically assisted analysis has shown that normal ROS-injured and cancerous human colon cells can be distinguished based on their unique vibrational properties. The research carried out proves that label-free Raman spectroscopy may play an important role in clinical diagnostics differentiation of normal and cancerous colon cells and may be a source of intraoperative information supporting histopathological analysis.

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