Rapid Determination of Fumonisin B1in Food Samples by Enzyme-Linked Immunosorbent Assay and Colloidal Gold Immunoassay

2006 ◽  
Vol 54 (7) ◽  
pp. 2491-2495 ◽  
Author(s):  
Shuo Wang ◽  
Ying Quan ◽  
Nanju Lee ◽  
Ivan R. Kennedy
Keyword(s):  
Immunosorbent Assay ◽  
Colloidal Gold ◽  
Rapid Determination ◽  
Food Samples ◽  
Enzyme Linked Immunosorbent Assay

A flow-based enzyme-linked immunosorbent assay on a polydimethylsiloxane microchip for the rapid determination of immunoglobulin A

Talanta ◽  
2006 ◽  
Vol 70 (1) ◽  
pp. 122-127 ◽  
Author(s):  
H NAKAJIMA ◽  
M YAGI ◽  
Y KUDO ◽  
T NAKAGAMA ◽  
T SHIMOSAKA ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Rapid Determination ◽  
Immunoglobulin A ◽  
Enzyme Linked Immunosorbent Assay

scholarly journals Rapid Determination of Immunoglobulin A in Human Saliva by Enzyme-Linked Immunosorbent Assay on a Micro Channel Chip

2005 ◽  
Vol 54 (9) ◽  
pp. 817-823 ◽  
Author(s):  
Hizuru NAKAJIMA ◽  
Hironori MASUDA ◽  
Satomi ISHINO ◽  
Tatsuro NAKAGAMA ◽  
Takuya SHIMOSAKA ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Rapid Determination ◽  
Immunoglobulin A ◽  
Human Saliva ◽  
Enzyme Linked Immunosorbent Assay ◽  
Micro Channel

Rapid quantitative determination of fentanyl in human urine and serum using a gold-based immunochromatographic strip sensor

2020 ◽  
Vol 8 (37) ◽  
pp. 8573-8584
Author(s):  
Xianlu Lei ◽  
Xinxin Xu ◽  
Liqiang Liu ◽  
Hua Kuang ◽  
Liguang Xu ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Quantitative Determination ◽  
Immunosorbent Assay ◽  
Human Urine ◽  
Colloidal Gold ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immunochromatographic Strip ◽  
Human Urine And Serum ◽  
Urine And Serum

In this study, an ultrasensitive monoclonal antibody (mAb) was prepared and used to develop an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and a colloidal gold-based immunochromatographic strip (CG-ICS) for the analysis of fentanyl in urine and serum.

scholarly journals Impacts of Extraction Methods in the Rapid Determination of Atrazine Residues in Foods using Supercritical Fluid Chromatography and Enzyme-Linked Immunosorbent Assay: Microwave Solvent vs. Supercritical Fluid Extractions

2005 ◽  
Vol 5 ◽  
pp. 11-19 ◽  
Author(s):  
Mohamed H. El-Saeid ◽  
Ijeoma Kanu ◽  
Ebere C. Anyanwu ◽  
Mahmoud A. Saleh
Keyword(s):  
Supercritical Fluid ◽  
Immunosorbent Assay ◽  
Supercritical Fluid Chromatography ◽  
Rapid Determination ◽  
Extraction Methods ◽  
Enzyme Linked Immunosorbent Assay ◽  
Food Markets ◽  
Regulatory Standards ◽  
Elisa Technique

It is an accepted fact that many food products that we eat today have the possibility of being contaminated by various chemicals used from planting to processing. These chemicals have been shown to cause illnesses for which some concerned government agencies have instituted regulatory mechanisms to minimize the risks and the effects on humans. It is for these concerns that reliable and accurate rapid determination techniques are needed to effect proper regulatory standards for the protection of people's nutritional health. This paper, therefore, reports the comparative evaluation of the extraction methods in the determination of atrazine (commonly used in agricultural as a herbicide) residues in foods using supercritical fluid chromatography (SFC) and enzyme-linked immunosorbent assay (ELISA) techniques. Supercritical fluid extraction (SFE) and microwave solvent extraction (MSE) methods were used to test samples of frozen vegetables, fruit juice, and jam from local food markets in Houston. Results showed a high recovery percentage of atrazine residues using supercritical fluid coupled with ELISA and SFC than with MSE. Comparatively, however, atrazine was detected 90.9 and 54.5% using SFC and ELISA techniques, respectively. ELISA technique was, however, less time consuming, lower in cost, and more sensitive with low detection limit of atrazine residues than SFC technique.

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