Monitoring and Estimation of the Kinetics Parameters in the Binding Process of Tannic Acid to Bovine Serum Albumin with Electrochemical Quartz Crystal Impedance System

2006 ◽  
Vol 54 (12) ◽  
pp. 4087-4094 ◽  
Author(s):  
Meiling Liu ◽  
Youyu Zhang ◽  
Qin Yang ◽  
Qingji Xie ◽  
Shouzhuo Yao
2019 ◽  
Vol 8 (1) ◽  
pp. 35-41 ◽  
Author(s):  
Lalu A. Didik ◽  
Yahdi Yahdi ◽  
Masruroh Masruroh

Polystyrene (PS) thin film with various concentration of mass (1%, 3%, and 5%) has been deposited onto Quartz Crystal Microbalance (QCM)  surface by a spin coating method. The purpose of this research is the improvement of Bovine Serum Albumin (BSA) immobilization into the QCM surface. The QCM resonance frequency has been investigated by means of Impedance Analyzer. The film thickness was calculated by the Sauerbrey equation. The surface roughness of the PS layer was observed by noncontact Topography Measuring System (TMS-1200). Calculation result using a Sauerbrey equation shows the thickness of the PS layer increases with the increasing PS concentration. The QCM/PS with 1% and 5% concentration of mass has rougher morphology and more frequency shifts caused by BSA injection compared to 3% concentration of PS


2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Ece Kilic ◽  
Marina V. Novoselova ◽  
Su Hui Lim ◽  
Nikolay A. Pyataev ◽  
Sergey I. Pinyaev ◽  
...  

Author(s):  
Barbara Jachimska ◽  
Karolina Tokarczyk ◽  
Marta Łapczyńska ◽  
Agnieszka Puciul-Malinowska ◽  
Szczepan Zapotoczny

2010 ◽  
Vol 169 (1-2) ◽  
pp. 117-122 ◽  
Author(s):  
Tzong-Jih Cheng ◽  
Hsien-Yi Hsiao ◽  
Chien-Yu Chung ◽  
Po-Chung Chen ◽  
Richie L. C. Chen

2010 ◽  
Vol 93-94 ◽  
pp. 583-586 ◽  
Author(s):  
Tippavan Hongkachern ◽  
Verawat Champreda ◽  
Toemsak Srikhirin ◽  
Thidarat Wangkam ◽  
Tanakorn Osotchan

The layer formation of bovine serum albumin (BSA) on a poly(styrene-co-maleic acid) (PSMA) surface was investigated by using quartz crystal microbalance (QCM) technique at various pH values. The formation of a BSA surface was examined by atomic force microscopy (AFM). To study the effect on the layer formation, the pH of solution was varied from 2 to 7.4 while the concentration of BSA was in the range of 0.01 to 5 mg/ml during the layer absorption. It was found that the BSA adsorption strongly depends on the pH of solution, and the concentration of BSA. The absorption layer occurred maximum at the pH value of 3.5 which resulted from the charge of PSMA and BSA molecules. The layer formation reached the saturate value at the concentration higher than 3 mg/ml. The molecular packing of the BSA layer at different pH values was determined by AFM and total mass change of QCM.


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