Cloning, Expression, and Characterization of Soluble Starch Synthase I cDNA from Taro (Colocasia esculentaVar.esculenta)

2005 ◽  
Vol 53 (20) ◽  
pp. 7985-7990 ◽  
Author(s):  
Da-Gin Lin ◽  
Chii-Ling Jeang
Keyword(s):  
Soluble Starch ◽  
Starch Synthase

scholarly journals Pathway of Cytosolic Starch Synthesis in the Model Glaucophyte Cyanophora paradoxa

2007 ◽  
Vol 7 (2) ◽  
pp. 247-257 ◽  
Author(s):  
Charlotte Plancke ◽  
Christophe Colleoni ◽  
Philippe Deschamps ◽  
David Dauvillée ◽  
Yasunori Nakamura ◽  
...  
Keyword(s):  
Starch Synthesis ◽  
Length Distribution ◽  
Soluble Starch ◽  
Land Plant ◽  
Starch Synthase ◽  
Cyanophora Paradoxa ◽  
Chain Length Distribution ◽  
Photosynthetic Eukaryotes ◽  
A Chain

ABSTRACT The nature of the cytoplasmic pathway of starch biosynthesis was investigated in the model glaucophyte Cyanophora paradoxa. The storage polysaccharide granules are shown to be composed of both amylose and amylopectin fractions, with a chain length distribution and crystalline organization similar to those of green algae and land plant starch. A preliminary characterization of the starch pathway demonstrates that Cyanophora paradoxa contains several UDP-glucose-utilizing soluble starch synthase activities related to those of the Rhodophyceae. In addition, Cyanophora paradoxa synthesizes amylose with a granule-bound starch synthase displaying a preference for UDP-glucose. A debranching enzyme of isoamylase specificity and multiple starch phosphorylases also are evidenced in the model glaucophyte. The picture emerging from our biochemical and molecular characterizations consists of the presence of a UDP-glucose-based pathway similar to that recently proposed for the red algae, the cryptophytes, and the alveolates. The correlative presence of isoamylase and starch among photosynthetic eukaryotes is discussed.

Molecular cloning and characterization of a gene encoding soluble starch synthase III (SSSIII) in Lotus (Nelumbo nucifera)

Biologia ◽  
2019 ◽  
Vol 75 (2) ◽  
pp. 279-288
Author(s):  
FengLin Zhu ◽  
Neng Cheng ◽  
Han Sun ◽  
Ying Diao ◽  
Zhongli Hu
Keyword(s):  
Molecular Cloning ◽  
Soluble Starch ◽  
Starch Synthase ◽  
Nelumbo Nucifera ◽  
Gene Encoding

scholarly journals Toward an understanding of the biogenesis of the starch granule. Determination of granule-bound and soluble starch synthase functions in amylopectin synthesis.

1994 ◽  
Vol 269 (40) ◽  
pp. 25150-25157
Author(s):  
M.L. Maddelein ◽  
N. Libessart ◽  
F. Bellanger ◽  
B. Delrue ◽  
C. D'Hulst ◽  
...  
Keyword(s):  
Starch Granule ◽  
Soluble Starch ◽  
Starch Synthase

scholarly journals QTL-seq identifies cooked grain elongation QTLs near soluble starch synthase and starch branching enzymes in rice (Oryza sativa L.)

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Siwaret Arikit ◽  
Samart Wanchana ◽  
Srisawat Khanthong ◽  
Chatree Saensuk ◽  
Tripop Thianthavon ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Soluble Starch ◽  
Starch Synthase ◽  
Starch Branching Enzymes ◽  
Branching Enzymes

Effects of Pore-Forming Agent on Characterization of NiO/YSZ Porous Anode for SOFC

2016 ◽  
Vol 848 ◽  
pp. 389-395
Author(s):  
Chag Ku Ding ◽  
Jing De Zhang ◽  
Ya Lei Liu ◽  
Jin Yan Gou ◽  
Jun Peng Luan
Keyword(s):  
Activated Carbon ◽  
Mechanical Strength ◽  
Carbon Particle ◽  
Soluble Starch ◽  
Stabilized Zirconia ◽  
Microstructural Characteristics ◽  
Mixing Ratios ◽  
Sintering Shrinkage ◽  
Porous Anode

A comparative study has been carried out on the effects of pore-forming agents on the microstructural characteristics and properties including porosity, microstructures, linear sintering shrinkage and mechanical strength of NiO/yttria stabilized zirconia (YSZ). Different pore-forming agents (soluble starch, graphite and activated carbon) were incorporated into NiO/YSZ at various mass mixing ratios (5%, 10% and 15%). The experimental results show that under the condition of same content, sintered samples using activated carbon as pore-forming agent had a higher porosity than that with identical content of the other two kinds of pore-forming agents. Sintered samples with 10.0 wt% activated carbon had a porosity of 35%. Section morphologies show that pores distributed uniformly and connected very well in the anode with activated carbon as pore-forming agent. The mechanical strength of these sintered samples with carbon as pore-forming agent was also higher. With the activated carbon particle size decreasing (from 100 mesh to 320 mesh), the open porosity of porous NiO/YSZ was reduced (from 31.7% to 30.9%). It may be concluded that The activated carbon is the better choice as pore-forming agent for NiO/YSZ anode.

scholarly journals Structure, Organization, and Chromosomal Location of the Gene Encoding a Form of Rice Soluble Starch Synthase

1995 ◽  
Vol 108 (2) ◽  
pp. 677-683 ◽  
Author(s):  
Ki. Tanaka ◽  
S. Ohnishi ◽  
N. Kishimoto ◽  
T. Kawasaki ◽  
T. Baba
Keyword(s):  
Chromosomal Location ◽  
Soluble Starch ◽  
Starch Synthase ◽  
Gene Encoding

scholarly journals Expression of a rice soluble starch synthase gene in transgenic wheat improves the grain yield under heat stress conditions

2018 ◽  
Vol 54 (3) ◽  
pp. 216-227 ◽  
Author(s):  
Bin Tian ◽  
Shyamal K. Talukder ◽  
Jianming Fu ◽  
Allan K. Fritz ◽  
Harold N. Trick
Keyword(s):  
Heat Stress ◽  
Grain Yield ◽  
Transgenic Wheat ◽  
Soluble Starch ◽  
Starch Synthase ◽  
Stress Conditions

Thermal Characteristics of Soluble Starch Synthase From Wheat Endosperm

1995 ◽  
Vol 22 (4) ◽  
pp. 703 ◽  
Author(s):  
CF Jenner ◽  
K Denyer ◽  
J Guerin
Keyword(s):  
Thermal Characteristics ◽  
Soluble Starch ◽  
Starch Synthase ◽  
Wheat Grain ◽  
Wheat Endosperm ◽  
Temperature Range ◽  
Higher Temperature ◽  
Temperature Responses

The aim of the work reported in this paper was to characterise the thermal responses of soluble starch synthase (SSS) extracted from the endosperm of the developing wheat grain. Using partially purified preparations of SSS, the reaction obeyed Michaelis-Menten kinetics with both substrates amylopectin and ADPglucose. Both the Vmax and the Km varied with temperature. Values for Vmax were higher at 45�C compared with 25�C. However, the Km values for both substrates were also higher at 45�C than at 25�C indicating that the affinity of the enzyme for its substrates was reduced at the higher temperature. Over the temperature range 15-45�C, the Km for arnylopectin was minimal at 20�C, and rose exponentially between 25 and 45�C. Kinetic analyses indicated that the reaction was sequential and that the substrates could bind to the enzyme in either order. At 25�C the binding of one substrate to the enzyme increased the affinity of the complex for the second substrate but at 45�C these effects were abolished. These thermal characteristics of SSS could explain certain important features of the temperature responses of starch deposition in the wheat grain in vivo.

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